Purified plasmid DNA from potential Reporter clones for later testing.

Test purified plasmid DNA for correct insert.

• Miniprep potential transformants containing Reporter plasmids for ctxB, tcpJ, tcpP, and tcpS
• Clone inoculated from tcpS plate did not show expected yellow phenotype. 2 additional clones from the tcpS plate were inoculated in Chloramphenicol broth overnight to be purified and tested on Monday.

Preparing cells for plasmid purification.