Team:Paris Saclay/Notebook/July/10

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[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
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==Modeling==
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"by Pierre, Juliette, Arnaud, Eugene"
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Discussion around differents modeling possibilities in concertation with Solenne.

Revision as of 14:04, 5 August 2014

Contents

Thursday 10th July

Lab work

1 - Plasmid DNA Extraction:

by Romain and Sean

Plasmids used:

  • BBa_B0015, dble ter
  • FNR_RBS_amilCP-Ter DH5αZ1

Protocol

2 - Transformation of competent cells E.coli

by Fabio and Maher

Competent strains used:

  • DH5α
  • MG1655
  • MG1655Z1
  • DH5α+ (given by Solenne)

(The strains DH5α, MG1655 and MG1655Z1 were prepared by Mathias and Mathieu)

BioBricks used:

  • BBa_J23100 (Amp R)(Dilutions: Concentrated, 0 and 10-1)
  • BBa_J23106 (Amp R)(Dilutions: Concentrated, 0 and 10-1)
  • BBa_J23114 (Amp R)(Dilutions: Concentrated, 0 and 10-1)

+ pET28a (Kan R)(Dilutions: 0, 10-1 and 10-2) (pET28a is a plasmid used to verify that the 4 strains are competent).

Negative control groups:

  • pET28a: without plasmid, nothing grows.
  • BBa_J23100, BBa_J23106, BBa_J23114: without the BioBrick, nothing grows.

Results:

  • pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well.
  • BBa_J23100, BBa_J23106, BBa_J23114: The strain DH5α+ grow well and the 3 others strains grow less. They have to be more concentrated.

Protocol

Back to the calendar

Modeling

"by Pierre, Juliette, Arnaud, Eugene"

Discussion around differents modeling possibilities in concertation with Solenne.

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/July/10"