Team:Cambridge-JIC/Notebook

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<div align="center"><a href="https://2014.igem.org/wiki/index.php?title=Team:Cambridge-JIC/Notebook&action=edit">Edit this page</a></div>
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<a href="https://2014.igem.org/Special:Upload">Use this page to upload pictures onto the wiki</a>
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<a href="https://2014.igem.org/Special:Upload">Use this page to upload pictures to the wiki</a>
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<div id="lab notebook" align="center">
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Lab notebook:
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Captain's log:  
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/CL_2014_7_16">2014 7 16</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_1">Week 1</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/CL_2014_7_17">2014 7 17</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_2">Week 2</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/CL_2014_7_18">2014 7 18</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_3">Week 3</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/CL_2014_7_21">2014 7 21</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_4">Week 4</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/CL_2014_7_22">2014 7 22</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_5">Week 5</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/CL_2014_7_23">2014 7 23</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_6">Week 6</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/CL_2014_7_24">2014 7 24</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_7">Week 7</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/CL_2014_7_25">2014 7 25</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_8">Week 8</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_9">Week 9</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_10">Week 10</a></li>
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<h3>First Ideas</h3>
 
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<h4> Mar-Cam-tia as a volatiles factory</h4>
 
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<p>Add metabolic pathways that produce perfumed volatiles (eg geraniol, limonene, geosmin). Or insect repellents/attractants such as E-beta-farnesene to repel aphids and attract ladybirds, or bombykol to attract moths from miles away.</p>
 
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<p>Production could be linked to circadian clock to change smell (or species of moth attracted) throughout the day -<strong> ie Mar-Cam-tia as a clock.</strong> There could be a link to Marchy's air pores.</p>
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<div id="constructs" align="center">
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Constructs:
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Constructs/progress_template">Construct Progress Template</li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Constructs/gal4_hap1gr_H2RBmRFP">Gal4-Hap1GR-H2RBmRFP</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Constructs/35S-HBRmRFP">35S-HBRmRFP</a></li>
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<h4>Mar-Cam-tia as a new form of LCD screen</h4>
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<p>If chromoprotein expression can be triggered in arbitrary cells by irradiating them with a far-red laser to induce the phytochrome signalling pathway in them, thalli could be written on with light and an image formed. Different lasers, different colour chromoproteins = full palette of laser-plant-pens.</p>
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<h4>Mar-Cam-tia as phytoELISA</h4>
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<p>An antigen receptor based on an antibody would be expressed on the surface of Marchantia, and a potentially novel signalling pathway (perhaps involving cAMP production or a tyrosine kinase dimer formation mechanism) engineered such that when the antigen of interest binds with the receptor, downstream events induce an obvious reporting process such as chromoprotein expression, luciferin-mediated glowing, morphological changes or the release of a characteristic smell. Qualitative disease testing with a product that replicates itself out of water and sunshine instead of a single use ELISA that costs $300.</p>
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<h4>Mar-Cam-tia as a reading lamp</h4>
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<p>Engineer the luciferin-luciferase system that the 2010 Cambridge team put into 'E. glowli' into Marchy, and show the dodgy kickstarter dude how it should really be done. This could be linked to the plant's circadian rhythm to ensure only nightime glowing.</p>
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<h4>Marchantia change-o-morpha</h4>
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<p>A morphological study of Marchy: Can we engineer shape? Can we disrupt the normal course of growth and shape formation? Maybe we can shape it like a cauliflower.</p>
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<h4>Spore removal</h4>
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<p>As is, spores are a biocontainment issue. If Marchy was less sexually prolific we would have less of a problem. It would be interesting and aesthetically pleasing to turn the gametophores into the readout of a potential biosensor.</p>
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Latest revision as of 15:47, 30 July 2014