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Team:Caltech/week6
From 2014.igem.org
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| - | <ul> | + | <b>Export Systems</b> |
| + | 3 of our 5 constructs were not properly cloned, so we are going to redo them: | ||
| + | <ul><li>PCRed out backbones for pTG002, pTG003, and pTG006 with overhangs</li> | ||
| + | <li>PCR product was then DpnI digested and PCR-purified. A gel was run with processed and unprocessed product to verify backbone creation</li> | ||
| + | <li>Gibson assembly of pTG002, pTG003, and pTG006 (pTG006 had 2 reactions: 1 with the backbone created last week, since it had been verified to work for cloning pTG005, and another with backbone created today)</li> | ||
| + | <li>Assemblies were transformed into JM109, plated on carbenicillin plates, and incubated overnight</li> | ||
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Revision as of 06:10, 22 July 2014
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