Team:HokkaidoU Japan/Notebook/H Stem/Replacement To 1A3

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<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li>
<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li>
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<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Estimation">Evaluation</a></li>
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<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Evaluation">Evaluation</a></li>
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      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
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pSB1A3 with EcoRI & PstI,
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pSB1A3 with EcoRI & PstI
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        R0010-stem-as-stem-B0015 with EcoRI & PstI
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<div class="step-recipe">
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R0010-stem-as-stem-B0015 with EcoRI & PstI
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      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
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0010-stem-as-stem-B0015 & pSB1A3
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Ligate 0010-stem-as-stem-B0015 with pSB1A3
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      <div class="step-recipe">
      <div class="step-recipe">
R0010-stem-as-stem-B0015(on pSB1A3)
R0010-stem-as-stem-B0015(on pSB1A3)
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        5.0&micro;L to DH5alpha;
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</div>
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<div class="step-recipe">
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5.0&micro;L to DH5alpha;
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Latest revision as of 15:28, 9 September 2015

Notebook
Lab Documents

H-Stem Vector Exchange to pSB1A3

  • Start

  • Mini-Prep

    R0010-stem-as-stem-B0015 (on pSB1A3)
  • PCR

    R0010-stem-as-stem-B0015(on pSB1C3) with 100up_F Tm:62.6℃ & 200dn_R Tm:66.6℃
  • Digestion

    pSB1A3 with EcoRI & PstI
    R0010-stem-as-stem-B0015 with EcoRI & PstI
  • Ligation

    Ligate 0010-stem-as-stem-B0015 with pSB1A3
  • Transformation

    R0010-stem-as-stem-B0015(on pSB1A3)
    5.0µL to DH5alpha;
  • Complete!