Team:HokkaidoU Japan/Notebook/H Stem/Replacement To 1A3

From 2014.igem.org

(Difference between revisions)
 
(5 intermediate revisions not shown)
Line 5: Line 5:
{{Team:HokkaidoU_Japan/LargeDropDown/CSS}}
{{Team:HokkaidoU_Japan/LargeDropDown/CSS}}
<html>
<html>
 +
<link href='http://fonts.googleapis.com/css?family=Merriweather' rel='stylesheet' type='text/css'>
<div id="background">
<div id="background">
<div id="background">
<div id="background">
Line 82: Line 83:
</ul>
</ul>
<ul>
<ul>
-
<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey">High-School</a></li>
+
<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey">High School</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey#Education">Education</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey#Education">Education</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey#Survey">Survey</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey#Survey">Survey</a></li>
Line 89: Line 90:
<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li>
<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li>
-
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Estimation">Evaluation</a></li>
+
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Evaluation">Evaluation</a></li>
</ul>
</ul>
</div>
</div>
Line 127: Line 128:
  <div class="step-tile">
  <div class="step-tile">
  <h2 class="step-title">
  <h2 class="step-title">
-
mini-Prep
+
Mini-Prep
</h2>
</h2>
  </div>
  </div>
Line 140: Line 141:
      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
-
1A3 Vector
+
R0010-stem-as-stem-B0015 (on pSB1A3)
 +
 
</div>
</div>
    </div>
    </div>
Line 161: Line 163:
      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
-
Plac-stem-as-stem-dT on 1C3 with 100up_F & 200dn_R
+
R0010-stem-as-stem-B0015(on pSB1C3) with 100up_F Tm:62.6&#8451; & 200dn_R Tm:66.6&#8451;
</div>
</div>
    </div>
    </div>
Line 182: Line 184:
      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
-
1A3 with EcoRI & PstI, Plac-stem-as-stem-dT with EcoRI & PstI
+
pSB1A3 with EcoRI & PstI
 +
</div>
 +
<div class="step-recipe">
 +
R0010-stem-as-stem-B0015 with EcoRI & PstI
 +
 
</div>
</div>
    </div>
    </div>
Line 203: Line 209:
      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
-
1A3 & Plac-stem-as-stem-dT
+
Ligate 0010-stem-as-stem-B0015 with pSB1A3
</div>
</div>
    </div>
    </div>
Line 224: Line 230:
      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
-
Plac-stem-as-stem-dT on 1A3
+
R0010-stem-as-stem-B0015(on pSB1A3)
 +
</div>
 +
<div class="step-recipe">
 +
5.0&micro;L to DH5alpha;
 +
 
</div>
</div>
    </div>
    </div>
Line 242: Line 252:
   <div id="footer-content">
   <div id="footer-content">
<div id="footer-logo">
<div id="footer-logo">
-
<a href="http://igemhokkaidou.com/"><img style="height:150px;position:relative;" src="https://static.igem.org/mediawiki/2014/3/39/HokkaidoU_logo_transparent.png"></a>
+
<a href="http://igemhokkaidou.wordpress.com"><img style="height:150px;position:relative;" src="https://static.igem.org/mediawiki/2014/3/39/HokkaidoU_logo_transparent.png"></a>
</div>
</div>
<div id="footer-twitter">
<div id="footer-twitter">

Latest revision as of 15:28, 9 September 2015

Notebook
Lab Documents

H-Stem Vector Exchange to pSB1A3

  • Start

  • Mini-Prep

    R0010-stem-as-stem-B0015 (on pSB1A3)
  • PCR

    R0010-stem-as-stem-B0015(on pSB1C3) with 100up_F Tm:62.6℃ & 200dn_R Tm:66.6℃
  • Digestion

    pSB1A3 with EcoRI & PstI
    R0010-stem-as-stem-B0015 with EcoRI & PstI
  • Ligation

    Ligate 0010-stem-as-stem-B0015 with pSB1A3
  • Transformation

    R0010-stem-as-stem-B0015(on pSB1A3)
    5.0µL to DH5alpha;
  • Complete!