Team:OUC-China

From 2014.igem.org

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Line 1: Line 1:
{{CSS/Main}}
{{CSS/Main}}
<html>
<html>
-
    <link href="https://2014.igem.org/Team:OUC-China/css/bootstrap.css?action=raw&ctype=text/css" rel="stylesheet" type="text/css" />
+
<link href="https://2014.igem.org/Team:OUC-China/css/bootstrap.css?action=raw&amp;ctype=text/css" rel="stylesheet" />
-
     <link href="https://2014.igem.org/Team:OUC-China/css/topsection.css?action=raw&ctype=text/css" rel="stylesheet" type="text/css" />
+
     <link href="https://2014.igem.org/Team:OUC-China/css/topsection.css?action=raw&ctype=text/css" rel="stylesheet" />
     <script src="https://2014.igem.org/Team:OUC-China/js/jquery.js?action=raw&ctype=text/javascript"></script>
     <script src="https://2014.igem.org/Team:OUC-China/js/jquery.js?action=raw&ctype=text/javascript"></script>
     <script src="https://2014.igem.org/Team:OUC-China/js/bootstrap.js?action=raw&ctype=text/javascript"></script>
     <script src="https://2014.igem.org/Team:OUC-China/js/bootstrap.js?action=raw&ctype=text/javascript"></script>
-
    <script src="https://2014.igem.org/Team:OUC-China/js/unslider.js?action=raw&ctype=text/javascript"></script>
+
<script type="text/javascript" src="https://2014.igem.org/Team:OUC-China/js/jquery.devrama.slider.js?action=raw&ctype=text/javascript"></script>
-
<style>
+
<script src="https://2014.igem.org/Team:OUC-China/js/unslider.js?action=raw&ctype=text/javascript"></script>
-
         #silder,#silder > ul, #silder > ul > li {
+
<style type="text/css">
 +
a:hover { text-decoration: none !important; }
 +
@charset "utf-8";
 +
/* CSS Document */
 +
body, div, p, ul, li, h1, h2, h3, h4, h5, h6 {
 +
margin: 0px;
 +
padding: 0px;
 +
font-family: "微软雅黑";
 +
}
 +
body{
 +
    min-width:1340px;
 +
}
 +
ul, ol {
 +
list-style: none;
 +
}
 +
img {
 +
display: block;
 +
position: absolute;
 +
}
 +
a {
 +
color: inherit;
 +
text-decoration: none;
 +
}
 +
@charset "utf-8";
 +
/* CSS Document */
 +
@keyframes bubleM {
 +
0% {
 +
margin-top:0px;
 +
margin-left:0px;
 +
}
 +
25% {
 +
margin-top:3px;
 +
margin-left:3px;
 +
}
 +
50% {
 +
margin-top:6px;
 +
margin-left:0px;
 +
}
 +
75% {
 +
margin-top:3px;
 +
margin-left:-3px;
 +
}
 +
100% {
 +
margin-top:0px;
 +
margin-left:0px;
 +
}
 +
}
 +
@-webkit-keyframes bubleM {
 +
0% {
 +
margin-top:0px;
 +
margin-left:0px;
 +
}
 +
25% {
 +
margin-top:3px;
 +
margin-left:3px;
 +
}
 +
50% {
 +
margin-top:6px;
 +
margin-left:0px;
 +
}
 +
75% {
 +
margin-top:3px;
 +
margin-left:-3px;
 +
}
 +
100% {
 +
margin-top:0px;
 +
margin-left:0px;
 +
}
 +
}
 +
.header {
 +
width: 1200px;
 +
height: 50px;
 +
margin: 0 auto;
 +
position: relative;
 +
}
 +
.header .igemLogo {
 +
transition: 0.5s;
 +
-moz-transition: 0.5s;
 +
-o-transition: 0.5s;
 +
-webkit-transition: 0.5s;
 +
}
 +
.header .igemLogo:hover {
 +
transform: rotate(360deg);
 +
-webkit-transform: rotate(360deg);
 +
-moz-transform: rotate(360deg);
 +
-o-transform: rotate(360deg);
 +
-ms-transform: rotate(360deg);
 +
}
 +
 
 +
.header .nav{
 +
top: 10px;
 +
left: 450px;
 +
}
 +
.header .Logo {
 +
width: 70px;
 +
height: 70px;
 +
background: url(https://static.igem.org/mediawiki/2014/f/fa/OUC-China_14Logo.png);
 +
position: absolute;
 +
right: 0;
 +
bottom: -10px;
 +
}
 +
.header .Logo ul {
 +
position: absolute;
 +
width: 250px;
 +
height: 150px;
 +
display: none;
 +
right: 0px;
 +
top: 70px;
 +
}
 +
.header .Logo li {
 +
height: 50px;
 +
width: 215px;
 +
line-height: 50px;
 +
font-size: 14px;
 +
background: #fff;
 +
margin: 10px 0 20px 0;
 +
position: relative;
 +
border-bottom-left-radius: 6px;
 +
border-top-left-radius: 6px;
 +
}
 +
.header .Logo li a {
 +
margin-left: 5px;
 +
}
 +
.header .Logo li img {
 +
position: absolute;
 +
right: -35px;
 +
top: -10px;
 +
}
 +
.neck {
 +
height: 512px;
 +
width: 100%;
 +
background: rgb(76,145,190);
 +
}
 +
.main {
 +
}
 +
.main .part1 {
 +
height: 480px;
 +
width: 100%;
 +
background: rgb(210,233,253);
 +
position: relative;
 +
}
 +
.main .part1 .waveUp {
 +
width: 100%;
 +
height: 130px;
 +
background: url(https://static.igem.org/mediawiki/2014/f/f0/WaveUp.png
 +
);
 +
position: absolute;
 +
bottom: 0;
 +
z-index: 0
 +
}
 +
.main .part1 .waveDown {
 +
width: 100%;
 +
height: 130px;
 +
background: url(https://static.igem.org/mediawiki/2014/6/63/WaveDown.png);
 +
position: absolute;
 +
bottom: 0;
 +
z-index: 3;
 +
}
 +
.main .part1 img {
 +
bottom: 60px;
 +
transition: 0.3s;
 +
-moz-transition: 0.3s;
 +
-o-transition: 0.3s;
 +
-webkit-transition: 0.3s;
 +
}
 +
.main .part1 img:hover {
 +
bottom: 80px;
 +
}
 +
.main .part1 .objLeft {
 +
left: 200px;
 +
}
 +
.main .part1 .objRight {
 +
right: 200px;
 +
}
 +
.main .part2 {
 +
width: 100%;
 +
height: 905px;
 +
background: rgb(96,159,210);
 +
position: relative;
 +
}
 +
.main .part2 ul li {
 +
position: absolute;
 +
display:none;
 +
}
 +
.main .part2 ul li p{
 +
display: block;
 +
position: absolute;
 +
width: 184px;
 +
color: rgb(49,129,194);
 +
text-align: center;
 +
font-size:24px;
 +
}
 +
.main .part2 .buble {
 +
animation-name: bubleM;
 +
animation-duration:1s;
 +
animation-timing-function: linear;
 +
animation-iteration-count: 10;
 +
animation-direction: alternate;
 +
animation-fill-mode: both;
 +
animation-play-state: running;
 +
-webkit-animation-name: bubleM;
 +
-webkit-animation-duration: 0.3s;
 +
-webkit-animation-timing-function: linear;
 +
-webkit-animation-iteration-count: 7;
 +
-webkit-animation-direction: alternate;
 +
-webkit-animation-fill-mode: both;
 +
-webkit-animation-play-state: running;
 +
}
 +
.delay1 {
 +
-moz-animation-delay: 0s;
 +
}
 +
.delay2 {
 +
-moz-animation-delay: 0.4s;
 +
}
 +
.delay3 {
 +
-moz-animation-delay: 0.8s;
 +
}
 +
.delay4 {
 +
-moz-animation-delay: 1.2s;
 +
}
 +
.delay5 {
 +
-moz-animation-delay: 1.6s;
 +
}
 +
.delay6 {
 +
-moz-animation-delay: 2s;
 +
}
 +
.main .part2 .lFish {
 +
left: 45px;
 +
bottom: -95px;
 +
}
 +
.main .part2 .rFish {
 +
right: 45px;
 +
bottom: -100px;
 +
}
 +
.footer {
 +
width: 100%;
 +
height: 220px;
 +
background: rgb(45,111,165);
 +
}
 +
@charset "utf-8";
 +
/* CSS Document */
 +
.nav {
 +
position: absolute;
 +
}
 +
.nav li {
 +
padding: 0 8px;
 +
float: left;
 +
position: relative;
 +
min-width: 80px;
 +
height: 40px;
 +
color: rgb(60,60,60);
 +
font-size: 24px;
 +
line-height: 40px;
 +
text-align: center;
 +
transition: 0.3s;
 +
-moz-transition: 0.3s;
 +
-o-transition: 0.3s;
 +
-webkit-transition: 0.3s;
 +
}
 +
.nav li:hover {
 +
background: rgb(146,205,253);
 +
color: #fff;
 +
}
 +
.nav .pMenu ul {
 +
display: none;
 +
position: absolute;
 +
top: 100%;
 +
left: 0px;
 +
overflow: hidden;
 +
border-bottom-left-radius: 6px;
 +
border-bottom-right-radius: 6px;
 +
}
 +
.nav .pMenu ul li {
 +
min-width: 220px;
 +
background: rgb(146,205,253);
 +
color: #fff;
 +
transition: 0.4s;
 +
-moz-transition: 0.4s;
 +
-o-transition: 0.4s;
 +
-webkit-transition: 0.4s;
 +
}
 +
.nav .pMenu ul li:hover {
 +
background: rgb(98,175,243);
 +
}
 +
.neck{ background-image:url(https://static.igem.org/mediawiki/2014/2/20/OUC-China_Index_Slide1.png)}
 +
.example-animation {
 +
color: #FFF;
 +
font-size: 60px;
 +
}
 +
header {
 +
            position: absolute;
 +
            width: 1020px;
 +
            margin: 0 auto;
 +
            z-index: 999;
 +
        }
 +
        header > ul {
 +
            list-style: none;
 +
            display: inline-block;
 +
            margin-left: 10px;
 +
            margin-right: 10px;float:left;
 +
            -webkit-transition: all ease 0.5s;
 +
        }
 +
        header > ul:hover {
 +
            margin-top: 0 !important;
 +
        }
 +
        header > ul > li {
 +
            width: 150px;
 +
            min-height: 50px;
 +
            padding: 10px;
 +
            background-color: #ffffff;
 +
        }
 +
        header > ul > li > a {
 +
            display: block;
 +
            font-size: 16px;
 +
            text-align: center;
 +
            cursor: pointer;
 +
            color: #1d89f7;
 +
        }
 +
        header > ul > li > a:hover {
 +
            color: #000000;
 +
            text-decoration: none;
 +
        }
 +
        header > ul > li:last-child {
 +
            height: 70px;
 +
            width: 70px;
 +
            border-radius: 50%;
 +
            margin-left: 40px;
 +
            margin-top: -10px;
 +
        }
 +
        header > ul > li:last-child > div {
 +
            width: 50px;
 +
            height: 50px;
 +
            border: 5px solid #1d89f7;
 +
            border-radius: 50%;
 +
        }
 +
        header > ul > li:last-child > div > div {
 +
            width: 0;
 +
            height: 0;
 +
            margin-left: 5px;
 +
            margin-top: 5px;
 +
            border-radius: 50%;
 +
            border: 15px solid #1d89f7;
 +
        }
 +
        .logo {
 +
            position: absolute;
 +
            top: 10px;
 +
            z-index: 10;
 +
        }
 +
#slider {
 +
            background-image: url(https://static.igem.org/mediawiki/2014/2/20/OUC-China_Index_Slide1.png);
 +
            background-repeat: no-repeat;
 +
            background-size: cover;
 +
        }
 +
         #slider,#slider > ul, #slider > ul > li {
             width:100%;
             width:100%;
-
             height:400px;
+
             height:615px;
         }
         }
-
         #silder > ul {
+
         #slider > ul {
             margin: 0;
             margin: 0;
             padding: 0;
             padding: 0;
         }
         }
-
         #silder > ul > li {
+
         #slider > ul > li {
             list-style: none;
             list-style: none;
         }
         }
-
             #silder > .dots {
+
             #slider > .dots {
                 position: relative;
                 position: relative;
                 left: 0;
                 left: 0;
Line 24: Line 377:
                 text-align: center;
                 text-align: center;
                 padding: 0;
                 padding: 0;
 +
                margin: 0 !important;
             }
             }
-
                 #silder > .dots > li {
+
                 #slider > .dots > li {
                     display: inline-block;
                     display: inline-block;
                     width: 10px;
                     width: 10px;
Line 39: Line 393:
                     transition: background .5s, opacity .5s;
                     transition: background .5s, opacity .5s;
                 }
                 }
-
                 #silder > .dots > li.active {
+
                 #slider > .dots > li.active {
                     background: #5b97fb;
                     background: #5b97fb;
                     opacity: 1;
                     opacity: 1;
                 }
                 }
-
         footer {
+
         @-webkit-keyframes CircleLogo {
-
             width: 100%;
+
             0% {
-
            height: 100px;
+
                transform: rotate(0deg);
-
             background-color: #4ca5fa;
+
             }
-
             margin-top: 50px;
+
            50% {
 +
                transform: rotate(180deg);
 +
             }
 +
            100% {
 +
                transform: rotate(360deg);
 +
            }
         }
         }
-
         footer p {
+
         @-moz-keyframes CircleLogo {
-
             color: #ffffff;
+
             0% {
-
             font-size: 14px;text-align: right;
+
                transform: rotate(0deg);
-
             padding-right: 20px;
+
             }
 +
            50% {
 +
                transform: rotate(180deg);
 +
             }
 +
            100% {
 +
                transform: rotate(360deg);
 +
            }
         }
         }
-
         footer p a {
+
         @-ms-keyframes CircleLogo {
-
             color: #ffffff;
+
             0% {
 +
                transform: rotate(0deg);
 +
            }
 +
            50% {
 +
                transform: rotate(180deg);
 +
            }
 +
            100% {
 +
                transform: rotate(360deg);
 +
            }
 +
        }
 +
        @keyframes CircleLogo {
 +
            0% {
 +
                transform: rotate(0deg);
 +
            }
 +
            50% {
 +
                transform: rotate(180deg);
 +
            }
 +
            100% {
 +
                transform: rotate(360deg);
 +
            }
         }
         }
-
         footer p a:hover {
+
</style>
-
             color: #c7f1ff;
+
<script>
 +
         if ($('.firstHeading')) $('.firstHeading').remove();
 +
    </script>
 +
<a href="https://igem.org/Main_Page"><img class="logo" src="https://static.igem.org/mediawiki/2014/c/c6/OUC-China_Content_IGEM.png" style="left:10px" /></a>
 +
<img class="logo" style="right:10px;animation: CircleLogo 20s linear infinite;-webkit-animation: CircleLogo 20s linear infinite;-moz-animation: CircleLogo 20s linear infinite;-ms-animation: CircleLogo 20s linear infinite" src="https://static.igem.org/mediawiki/2014/0/00/OUC_China_Content_Team.png" />
 +
    <header>
 +
        <ul>
 +
             <li style="border-bottom-left-radius:5px;border-bottom-right-radius:5px"><a href="#" style="font-size:22px">HOME</a></li>
 +
            <li><div><div></div></div></li>
 +
        </ul>
 +
        <ul style="margin-top:-260px">
 +
            <li><a href="https://2014.igem.org/Team:OUC-China/Team">Members</a></li>
 +
            <li><a href="https://2014.igem.org/Team:OUC-China/Team_Instructor">Instructor</a></li>
 +
            <li><a href="https://2014.igem.org/Team:OUC-China/Team_Acknowledgment">Acknowledgment</a></li>
 +
            <li><a href="https://2014.igem.org/Team:OUC-China/Team_Lab">Lab</a></li>
 +
            <li><a href="https://igem.org/Team.cgi?year=2014&team_name=OUC-China">Official Team Profile</a></li>
 +
            <li style="border-bottom-left-radius:5px;border-bottom-right-radius:5px"><a style ="font-size:22px">TEAM</a></li>
 +
            <li><div><div></div></div></li>
 +
        </ul>
 +
        <ul style="margin-top:-325px">
 +
            <li><a href="https://2014.igem.org/Team:OUC-China/Project">Overview</a></li>
 +
            <li><a href="https://2014.igem.org/Team:OUC-China/Project_Background">Background</a></li>
 +
            <li><a href="https://2014.igem.org/Team:OUC-China/Project_Design">Design</a></li>
 +
            <li><a href="https://2014.igem.org/Team:OUC-China/Project_Result">Result</a></li>
 +
            <li><a href="https://2014.igem.org/Team:OUC-China/Project_Future">Future</a></li>
 +
            <li><a href="https://2014.igem.org/Team:OUC-China/Project_Modeling">Modeling</a></li>
 +
            <li><a href="https://2014.igem.org/Team:OUC-China/Project_Policy_Practise">Policy&Practice</a></li>
 +
            <li style="border-bottom-left-radius:5px;border-bottom-right-radius:5px"><a style="font-size:22px">PROJECT</a></li>
 +
            <li><div><div></div></div></li>
 +
        </ul>
 +
        <ul style="margin-top:-90px">
 +
            <li><a href="https://2014.igem.org/Team:OUC-China/Judging">Biobrick</a></li>
 +
            <li><a href="https://2014.igem.org/Team:OUC-China/Judging_Achievment">Achievement</a></li>
 +
            <li style="border-bottom-left-radius:5px;border-bottom-right-radius:5px"><a style="font-size:22px">JUDGING</a></li>
 +
            <li><div><div></div></div></li>
 +
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            <li><a href="https://2014.igem.org/Team:OUC-China/Safety">Biosafety</a></li>
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                            <li><a href="https://2014.igem.org/Team:OUC-China/Safety_Transfection">Transfection</a></li>
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  <div class = "part1">
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<h2 class="text-primary" style="width: 960px;margin:0 auto;padding-top:10px;">Introduction</h2>
 +
<p style="width: 960px;margin:0 auto;font-size: 19px;padding-top:12px;">Current researches usually transfer exogenous DNA into prokaryotic cells and eukaryotic cells, while OUC-China iGEM team has designed an innovative method to carry exogenous DNA from prokaryote to eukaryota more efficiently. Eventually we have applied the method to studies of the model organism, zebrafish. <br />
 +
We have constructed the fusion protein that can bind and protect plasmids, with the ability of helping plasmids transfect into eukaryotic cells in high efficiency.</p>
 +
<a href="https://2014.igem.org/Team:OUC-China/Project" class="btn btn-primary btn-lg" style="display:block;margin:40px auto;width:200px;position:relative;color: #ffffff;">Learn More</a>
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        <p style="top: 200px; left: 70px;"><a href="https://2014.igem.org/Team:OUC-China/Project_Design#Double_Plamisd">The Double Plasmid System</a></p>
 +
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        <p style="top: 275px; left: 86px;"><a href="https://2014.igem.org/Team:OUC-China/Project_Design#Transfection">Transfection</a></p>
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 +
        <p style="top: 246px; left: 67px;"><a href="https://2014.igem.org/Team:OUC-China/Project_Modeling">Modeling</a></p>
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 +
        <p style="top: 232px; left: 59px;"><a href="https://2014.igem.org/Team:OUC-China/Judging">Judging</a></p>
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        <p style="top: 267px; left: 74px;"><a href="https://2014.igem.org/Team:OUC-China/Safety">Safety</a></p>
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        <p style="top: 231px; left: 69px;"><a href="https://2014.igem.org/Team:OUC-China/Project_Policy_Practise">Policy&Practise </a></p>
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Latest revision as of 03:59, 18 October 2014

Introduction

Current researches usually transfer exogenous DNA into prokaryotic cells and eukaryotic cells, while OUC-China iGEM team has designed an innovative method to carry exogenous DNA from prokaryote to eukaryota more efficiently. Eventually we have applied the method to studies of the model organism, zebrafish.
We have constructed the fusion protein that can bind and protect plasmids, with the ability of helping plasmids transfect into eukaryotic cells in high efficiency.

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