Team:British Columbia/Notebook/Protocols
From 2014.igem.org
(Difference between revisions)
AnnaMüller (Talk | contribs) (Created page with "{{:Team:British_Columbia/Templates/MainHeader}} <html> <div class="container"> <div class="page-header"> <h1>Protocols</h1> <p> <ul style="list...") |
|||
(3 intermediate revisions not shown) | |||
Line 8: | Line 8: | ||
<ul style="list-style: none; "> | <ul style="list-style: none; "> | ||
- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/PCR">PCR</a> </font> </b> | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/PCR">PCR</a> </font> </b> |
</li> | </li> | ||
Instruction for colony PCR and regular PCR | Instruction for colony PCR and regular PCR | ||
<br></br> | <br></br> | ||
- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/ | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/gelverification">Gel Verification</a> </font> </b> |
</li> | </li> | ||
Make a gel and conduct gel elecrophoresis | Make a gel and conduct gel elecrophoresis | ||
Line 18: | Line 18: | ||
<br></br> | <br></br> | ||
- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/Biobrickdigest">BioBrick Restriction Digest</a> </font> </b> | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/Biobrickdigest">BioBrick Restriction Digest</a> </font> </b> |
</li> | </li> | ||
Restriction digest with BioBrick plasmid an inserts | Restriction digest with BioBrick plasmid an inserts | ||
<br></br> | <br></br> | ||
- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/ligation">BioBrick Ligation</a> </font> </b> | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/ligation">BioBrick Ligation</a> </font> </b> |
</li> | </li> | ||
Ligate Biobrick insert to vector | Ligate Biobrick insert to vector | ||
<br></br> | <br></br> | ||
- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/plates">Making Plates</a> </font> </b> | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/plates">Making Plates</a> </font> </b> |
</li> | </li> | ||
Make LB plates with antibiotics | Make LB plates with antibiotics | ||
<br></br> | <br></br> | ||
- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/media">Liquid media</a> </font> </b> | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/media">Liquid media</a> </font> </b> |
</li> | </li> | ||
Make liquid LB media | Make liquid LB media | ||
<br></br> | <br></br> | ||
- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/compcells">Competent cells</a> </font> </b> | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/compcells">Competent cells</a> </font> </b> |
</li> | </li> | ||
Preparation of chemically competent cells | Preparation of chemically competent cells | ||
<br></br> | <br></br> | ||
- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/transformation">Transformation</a> </font> </b> | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/transformation">Transformation</a> </font> </b> |
</li> | </li> | ||
- | Transformation of | + | Transformation of plasmid DNA into chemically competent cells |
+ | |||
<br></br> | <br></br> | ||
- | <li> <b><font size="5"><a href=" | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/AnnealedOligonucleotides">Assembling Parts from Oligonucleotides</a> </font> </b> |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
</li> | </li> | ||
Assemble short parts from annealed oligonucleotides | Assemble short parts from annealed oligonucleotides | ||
<br></br> | <br></br> | ||
- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/MultisiteMutagenesis">(Multi) Site Directed Mutagenesis</a> </font> </b> | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/MultisiteMutagenesis">(Multi) Site Directed Mutagenesis</a> </font> </b> |
</li> | </li> | ||
Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations. | Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations. | ||
<br></br> | <br></br> | ||
- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/T4PNK">Oligonucleotide Phosphorylation</a> </font> </b> | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/T4PNK">Oligonucleotide Phosphorylation</a> </font> </b> |
</li> | </li> | ||
Phosphorylate oligonucleotides prior to ligation | Phosphorylate oligonucleotides prior to ligation | ||
<br></br> | <br></br> | ||
- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/ElectrocompCells">Electrocompetent Cell Production</a> </font> </b> | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/ElectrocompCells">Electrocompetent Cell Production</a> </font> </b> |
</li> | </li> | ||
Make electrocompetent cells | Make electrocompetent cells | ||
<br></br> | <br></br> | ||
- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/ | + | |
+ | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/BiopanningAssay">Biopanning Assay</a> </font> </b> | ||
</li> | </li> | ||
- | + | Affinity Selection Technique | |
- | < | + | <br> |
</ul> | </ul> |
Latest revision as of 03:58, 18 October 2014
Protocols
- PCR Instruction for colony PCR and regular PCR
- Gel Verification Make a gel and conduct gel elecrophoresis
- BioBrick Restriction Digest Restriction digest with BioBrick plasmid an inserts
- BioBrick Ligation Ligate Biobrick insert to vector
- Making Plates Make LB plates with antibiotics
- Liquid media Make liquid LB media
- Competent cells Preparation of chemically competent cells
- Transformation Transformation of plasmid DNA into chemically competent cells
- Assembling Parts from Oligonucleotides Assemble short parts from annealed oligonucleotides
- (Multi) Site Directed Mutagenesis Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.
- Oligonucleotide Phosphorylation Phosphorylate oligonucleotides prior to ligation
- Electrocompetent Cell Production Make electrocompetent cells
- Biopanning Assay Affinity Selection Technique