Team:EPF Lausanne

From 2014.igem.org

(Difference between revisions)
 
(23 intermediate revisions not shown)
Line 18: Line 18:
         <span class="icon-bar"></span>
         <span class="icon-bar"></span>
       </button>
       </button>
-
       <a class="navbar-brand" href="https://2014.igem.org/Team:EPF_Lausanne"><img src="https://static.igem.org/mediawiki/2014/d/dc/LogoEPFL.png" alt="" /></a>
+
       <a class="navbar-brand" href="https://igem.org/Main_Page" target="_blank"><img src="https://static.igem.org/mediawiki/2014/d/dc/LogoEPFL.png" alt="" /></a>
</a>
</a>
     </div>
     </div>
Line 118: Line 118:
       <div class="item">
       <div class="item">
-
<a href="https://2014.igem.org/Team:EPF_Lausanne/HumanPractice">
+
<a target="_blank" href="https://2014.igem.org/Team:EPF_Lausanne/HumanPractice">
         <img src="https://static.igem.org/mediawiki/2014/a/ad/Citations_carousel.png" alt="" id="slide5">
         <img src="https://static.igem.org/mediawiki/2014/a/ad/Citations_carousel.png" alt="" id="slide5">
</a>
</a>
Line 151: Line 151:
<br />
<br />
-
<img src="https://static.igem.org/mediawiki/2014/0/0f/Interaction_test_11_cyan_white_bg_bigger.gif" alt="EPFL_interaction_IFP_cartoon" height="330" class="img-left img-border" /> <figcaption><p>Association of IFP1.4 split fragments</p></figcaption>
+
<div class="img-left pull-left cntr"><img src="https://static.igem.org/mediawiki/2014/0/0f/Interaction_test_11_cyan_white_bg_bigger.gif" alt="EPFL_interaction_IFP_cartoon" class="img-border" style="margin-top: 0px;" height="330" /><br />
 +
<figcaption class="cntr">Association of split IFP 1.4 fragments</figcaption></div>
</div>
</div>
<p class="lead text-justify">
<p class="lead text-justify">
-
The 2014 EPFL iGEM team has been working on showing that biologically engineered organisms can detect and process signals quickly and efficiently. With this in mind, our team brought forward a novel idea: combining protein complementation techniques with biosensors to achieve fast spatiotemporal analysis of cell responses to stimuli. To restate this in an easier way, we fused complementary reporter protein fragments to interacting proteins. The presence of a given stimulus leads to the interaction of the proteins of interest thus allowing the fused split complements to re-acquire their functional conformation and emit signal. We thereby are able to detect signal dynamics by relying on much faster post-transcriptional modifications rather than slow traditional reporter transcription.  
+
The 2014 EPFL iGEM team has been working on showing that biologically engineered organisms can detect and process signals quickly and efficiently. With this in mind, our team brought forward a novel idea: combining protein complementation techniques with biosensors to achieve fast spatiotemporal analysis of cell responses to stimuli. In other words, we fused complementary reporter protein fragments to interacting proteins. The presence of a given stimulus leads to the interaction of the proteins of interest thus allowing the fused split complements to re-acquire their functional conformation and emit signal. We thereby are able to detect signal dynamics by relying on much faster post-transcriptional modifications rather than slow traditional reporter transcription.
<!--
<!--
Line 323: Line 324:
       <div class="back">
       <div class="back">
         <!-- back content -->
         <!-- back content -->
-
   <p class="lead">Our project is well suited to show the general public the power of synthetic biology. Find out how we introduced this domain to the younger generation, and how they developed their own mini iGEM projects to tackle everyday problems with enthusiasm and fantasy.</p>
+
   <p class="lead">Our project is well suited to show the general public the power of synthetic biology. Find out how we introduced this domain to the younger generation, and how they developed their own mini iGEM projects to tackle everyday problems with enthusiasm and creativity.</p>
       </div>
       </div>
     </div>
     </div>
Line 344: Line 345:
       <div class="back">
       <div class="back">
         <!-- back content -->
         <!-- back content -->
-
<p class="lead">A tiny clever device that requires only small amounts of cells and provides an enclosed environment to culture Genetically Modified Organisms: discover how microfluidics can <br/> improve biosafety!</p>
+
<p class="lead">The first microfluidic design that provides<br /> total on-chip waste decontamination: discover<br /> how we tackled biosafety issues by<br /> engineering an awesome device!</p>
     </div>
     </div>
     </div>
     </div>
Line 363: Line 364:
<p>We are a group of 13 students from the faculties of Life Sciences & Technologies and Computer Sciences, </br>and are supervised by 2 EPFL professors, 1 Lecturer and 5 PhD students.</p></div>
<p>We are a group of 13 students from the faculties of Life Sciences & Technologies and Computer Sciences, </br>and are supervised by 2 EPFL professors, 1 Lecturer and 5 PhD students.</p></div>
-
<a href="https://2014.igem.org/Team:EPF_Lausanne/Team"><img src="https://static.igem.org/mediawiki/2014/2/2c/Team_pic_sitting.jpg" alt="the team's students"></a>
+
<a href="https://2014.igem.org/Team:EPF_Lausanne/Team"><img src="https://static.igem.org/mediawiki/2014/2/2c/Team_pic_sitting.jpg" alt="the team's students" class="img-left img-border"></a>

Latest revision as of 03:50, 18 October 2014

Our project in a nutshell




EPFL_interaction_IFP_cartoon
Association of split IFP 1.4 fragments

The 2014 EPFL iGEM team has been working on showing that biologically engineered organisms can detect and process signals quickly and efficiently. With this in mind, our team brought forward a novel idea: combining protein complementation techniques with biosensors to achieve fast spatiotemporal analysis of cell responses to stimuli. In other words, we fused complementary reporter protein fragments to interacting proteins. The presence of a given stimulus leads to the interaction of the proteins of interest thus allowing the fused split complements to re-acquire their functional conformation and emit signal. We thereby are able to detect signal dynamics by relying on much faster post-transcriptional modifications rather than slow traditional reporter transcription.

As a proof-of-concept, we aimed to develop the first BioPad: a biological trackpad made of a microfluidic chip, touch-responsive organisms and a signal detector. To make our organisms touch-sensitive, we engineering two stress-related pathways in E. coli and S. cerevisiae. As for the reporter proteins, we worked mainly with fluorescent proteins but also implemented a split luciferase complementation assay. To learn more about the various components of our project, check out our overview section, as well as the different parts submitted by our team. If you are a judge, you might also be interested in our results page, our data page and our judging form.

MEET OUR TEAM

We are a group of 13 students from the faculties of Life Sciences & Technologies and Computer Sciences,
and are supervised by 2 EPFL professors, 1 Lecturer and 5 PhD students.

the team's students

Sponsors