Team:Jilin China/Outline

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<h2>May to July, 2014</h2>
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<h2>May, 2014</h2>
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<li>MlrA gene coding optimization（codon of lactic acid bacteria）</li>
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<li>Data query and project theme select </li>
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<li>MlrA gene synthesis(synthesis by pieces)</li>
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<li>Experiment scheme design </li>
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<li>Sequence analysis and discussion about whether it can be split</li>
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<li>Codon optimization of MlrA gene by Lactococcus lactis codons）</li>
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<li>Synthetic primer by company (33 pieces in total)</li>
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<li>MlrA gene sequence design and split</li>
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<li>Repeat PCR for many times, recovery them and then get the complete gene product</li>
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<li>RFP-Mlr-GFP sequence design and split</li></ol>
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<li>Sub cloning vector and sequenced</li>
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<h2>June, 2014</h2>
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<li>Try to express by E.coli and analyze the effect of this protein</li>
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<li>Express by lactic acid bacteria</li></ol>
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<h2>July to Sept, 2014</h2>
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<li>Discussion about many possible paths include MC-LR</li>
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<li>MlrA gene synthesis and sequence</li>
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<li>Try cloning Pseudomonas  natural promoter.(non-coding sequences in Mlr enzyme series )</li>
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<li>RFP-Mlr-GFP gene synthesis and sequence</li></ol>
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<li>Synthesis by pieces then got complete product.</li>
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<h2>July, 2014</h2>
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<li>The gene transformed into E.coli and verify its functions.</li>
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<li>Transform this gene by many ways into lactic acid bacteria and verify the function of promoter.</li></ol>
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<li>MlrA gene expression and detection</li>
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<li>RFP-Mlr-GFP gene expression and detection</li>
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<li>Screening of microcystin LR sensitive promoter</li></ol>
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<h2>August, 2014</h2>
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<li>construction of the recombinant vector pMG-mlr</li>
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<li>Recombinant vector was transformed into Escherichia coli</li>
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<li>GFP-mlrA expression by mlr promoter and detection in Escherichia coli</li>
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<li>Detection of microcystins LR degradation ability in water by Recombinant Escherichia coli</li></ol>
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<h2>September, 2014</h2>
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<li>Recombinant vector was transformed into Lactococcus lactis</li>
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<li>GFP-mlrA expression by mlr promoter and detection in Lactococcus lactis</li>
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<li>Detection of microcystins LR degradation ability in water by Recombinant Lactococcus lactis</li></ol>
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<h2>October, 2014</h2>
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<li>Detection of microcystins LR degradation ability in the natural environment by Recombinant Escherichia coli</li>
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<li>Detection of microcystins LR degradation ability in the natural environment by Recombinant Lactococcus lactis</li>
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Latest revision as of 03:35, 18 October 2014

Welcome!
Team Jilin_China

May, 2014

  1. Data query and project theme select
  2. Experiment scheme design
  3. Codon optimization of MlrA gene by Lactococcus lactis codons）
  4. MlrA gene sequence design and split
  5. RFP-Mlr-GFP sequence design and split

June, 2014

  1. MlrA gene synthesis and sequence
  2. RFP-Mlr-GFP gene synthesis and sequence

July, 2014

  1. MlrA gene expression and detection
  2. RFP-Mlr-GFP gene expression and detection
  3. Screening of microcystin LR sensitive promoter

August, 2014

  1. construction of the recombinant vector pMG-mlr
  2. Recombinant vector was transformed into Escherichia coli
  3. GFP-mlrA expression by mlr promoter and detection in Escherichia coli
  4. Detection of microcystins LR degradation ability in water by Recombinant Escherichia coli

September, 2014

  1. Recombinant vector was transformed into Lactococcus lactis
  2. GFP-mlrA expression by mlr promoter and detection in Lactococcus lactis
  3. Detection of microcystins LR degradation ability in water by Recombinant Lactococcus lactis

October, 2014

  1. Detection of microcystins LR degradation ability in the natural environment by Recombinant Escherichia coli
  2. Detection of microcystins LR degradation ability in the natural environment by Recombinant Lactococcus lactis

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