From 2014.igem.org
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Revision as of 03:21, 18 October 2014
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Welcome!
Team Jilin_China
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May, 2014
- Data query and project theme select
- Experiment scheme design
- Codon optimization of MlrA gene by lactic acid bacteria codons）
- MlrA gene sequence design and split
- RFP-Mlr-GFP sequence design and split
June, 2014
- MlrA gene synthesis and sequence
- RFP-Mlr-GFP gene synthesis and sequence
July, 2014
- MlrA gene expression and detection
- RFP-Mlr-GFP gene expression and detection
- Screening of microcystin LR sensitive promoter
August, 2014
- construction of the recombinant vector pMG-mlr
- construction of the recombinant vector pMG-mlr
- Synthetic primer by company (33 pieces in total)
- Repeat PCR for many times, recovery them and then get the complete gene product
- Sub cloning vector and sequenced
- Try to express by E.coli and analyze the effect of this protein
- Express by lactic acid bacteria
July to Sept, 2014
- Discussion about many possible paths include MC-LR
- Try cloning Pseudomonas natural promoter.(non-coding sequences in Mlr enzyme series )
- Synthesis by pieces then got complete product.
- The gene transformed into E.coli and verify its functions.
- Transform this gene by many ways into lactic acid bacteria and verify the function of promoter.
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