Team:OUC-China/Judging

From 2014.igem.org

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<h1>Biobrick</h1>
<h1>Biobrick</h1>
-
       <p >Origin of transfer for the RP4-plasmid nic region</p>
+
       <h4 class="text-primary divide">Origin of transfer for the RP4-plasmid nic region</h4>
       <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439000">BBa_K1439000</a></p>  
       <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439000">BBa_K1439000</a></p>  
       <p>Type: conjugation</p>
       <p>Type: conjugation</p>
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       <p>Description/Usage: OriTRP4,the RP4 plasmid nic region,is where the relaxosome nicks the plasmid and conjugative transfer by RP4 plasmid machinery begins.</p>
       <p>Description/Usage: OriTRP4,the RP4 plasmid nic region,is where the relaxosome nicks the plasmid and conjugative transfer by RP4 plasmid machinery begins.</p>
       <p> </p>
       <p> </p>
-
       <p>[OriTRP4]+[RFP]</p>
+
       <h4 class="text-primary divide">[OriTRP4]+[RFP]</h4>
       <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439001">BBa_K1439001</a></p>
       <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439001">BBa_K1439001</a></p>
       <p>Type: Composite</p>
       <p>Type: Composite</p>
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       <p>Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTRP4. Used to test plasmid mobility.</p>
       <p>Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTRP4. Used to test plasmid mobility.</p>
       <p></p>
       <p></p>
-
       <p class="text-primary divide">[OriTR]+[RFP]</p>
+
       <h4 class="text-primary divide">[OriTR]+[RFP]</h4>
       <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439002">BBa_K1439002</a></p>
       <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439002">BBa_K1439002</a></p>
       <p>Type: Composite</p>
       <p>Type: Composite</p>
Line 214: Line 214:
       <p></p>
       <p></p>
       <p class="text-primary"> </p>
       <p class="text-primary"> </p>
-
       <p class="text-primary divide">hEF1a_Cas9-VP16 MammoBlock</p>
+
       <h4 class="text-primary divide">hEF1a_Cas9-VP16 MammoBlock</h4>
       <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439003">BBa_K1179003</a></p>
       <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439003">BBa_K1179003</a></p>
       <p>Type: Generator</p>
       <p>Type: Generator</p>
<p>Length: 5141bp</p>
<p>Length: 5141bp</p>
<p>Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with guide RNA binding sites upstream.</p>
<p>Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with guide RNA binding sites upstream.</p>
-
<p class="text-primary divide">TAT-H4</p>
+
<h4 class="text-primary divide">TAT-H4</h4>
<p>ID: <a href="http://parts.igem.org/Part:BBa_K1439004">BBa_K1439004</a></p>
<p>ID: <a href="http://parts.igem.org/Part:BBa_K1439004">BBa_K1439004</a></p>
<p>Type: Coding</p>
<p>Type: Coding</p>
<p>Length:393bp</p>
<p>Length:393bp</p>
<p>Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte.</p>
<p>Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte.</p>
-
<p class="text-primary divide">TAT-PTD</p>
+
<h4 class="text-primary divide">TAT-PTD</h4>
<p>ID: <a href="http://parts.igem.org/Part:BBa_K1439005">BBa_K1439005</a></p>
<p>ID: <a href="http://parts.igem.org/Part:BBa_K1439005">BBa_K1439005</a></p>
<p>Type: Coding</p>
<p>Type: Coding</p>
<p>Length:33bp</p>
<p>Length:33bp</p>
<p>Description/Usage: This part codes the expression of the TAT-PTD that is used in helping the plasmid transfect into the eukaryocyte.</p>
<p>Description/Usage: This part codes the expression of the TAT-PTD that is used in helping the plasmid transfect into the eukaryocyte.</p>
-
<p class="text-primary divide">Histone H4</p>
+
<h4 class="text-primary divide">Histone H4</h4>
<p>ID: <a href="http://parts.igem.org/Part:BBa_K1439006">BBa_K1439006</a></p>
<p>ID: <a href="http://parts.igem.org/Part:BBa_K1439006">BBa_K1439006</a></p>
<p>Type: Coding</p>
<p>Type: Coding</p>
<p>Length:312bp</p>
<p>Length:312bp</p>
<p>Description/Usage: This part codes the expression of the Histone H4 that is used in combing and protecting the plasmid in bacterium.</p>
<p>Description/Usage: This part codes the expression of the Histone H4 that is used in combing and protecting the plasmid in bacterium.</p>
-
<p class="text-primary divide">CMV promoter</p>
+
<h4 class="text-primary divide">CMV promoter</h4>
<p>ID: <a href="http://parts.igem.org/Part:BBa_K1439007">BBa_K1439007</a></p>
<p>ID: <a href="http://parts.igem.org/Part:BBa_K1439007">BBa_K1439007</a></p>
<p>Type: Regulatory</p>
<p>Type: Regulatory</p>
<p>Length:588bp</p>
<p>Length:588bp</p>
<p>Description/Usage: We use CMV to improve the expression of TAT:H4 and the efficiency of transfection.</p>
<p>Description/Usage: We use CMV to improve the expression of TAT:H4 and the efficiency of transfection.</p>
-
<p class="text-primary divide">TAT-H4-B0015</p>
+
<h4 class="text-primary divide">TAT-H4-B0015</h4>
<p>ID: <a href="http://parts.igem.org/Part:BBa_K1439008">BBa_K1439008</a></p>
<p>ID: <a href="http://parts.igem.org/Part:BBa_K1439008">BBa_K1439008</a></p>
<p>Type: Composite</p>
<p>Type: Composite</p>
<p>Length:530bp</p>
<p>Length:530bp</p>
<p>Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte. It has a terminator and can be added different promoters.</p>
<p>Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte. It has a terminator and can be added different promoters.</p>
-
<p class="text-primary divide">Lysis device induced by L-arabinose</p>
+
<h4 class="text-primary divide">Lysis device induced by L-arabinose</h4>
       <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439009">BBa_K1439009</a></p>
       <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439009">BBa_K1439009</a></p>
       <p>Type: Device</p>
       <p>Type: Device</p>
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       <p>Description/Usage:This part encodes the expression of the lysis protein that is induced by L-arabinose mobility.</p>
       <p>Description/Usage:This part encodes the expression of the lysis protein that is induced by L-arabinose mobility.</p>
       <p></p>
       <p></p>
-
       <p class="text-primary divide">Lysis device induced by aTc</p>
+
       <h4 class="text-primary divide">Lysis device induced by aTc</h4>
       <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439010">BBa_K1439010</a></p>
       <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439010">BBa_K1439010</a></p>
       <p>Type: Device</p>
       <p>Type: Device</p>

Latest revision as of 03:07, 18 October 2014

Biobrick

Origin of transfer for the RP4-plasmid nic region

ID: BBa_K1439000

Type: conjugation

Length:350bp

Description/Usage: OriTRP4,the RP4 plasmid nic region,is where the relaxosome nicks the plasmid and conjugative transfer by RP4 plasmid machinery begins.

[OriTRP4]+[RFP]

ID: BBa_K1439001

Type: Composite

Length:1427bp

Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTRP4. Used to test plasmid mobility.

[OriTR]+[RFP]

ID: BBa_K1439002

Type: Composite

Length:1448bp

Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.

hEF1a_Cas9-VP16 MammoBlock

ID: BBa_K1179003

Type: Generator

Length: 5141bp

Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with guide RNA binding sites upstream.

TAT-H4

ID: BBa_K1439004

Type: Coding

Length:393bp

Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte.

TAT-PTD

ID: BBa_K1439005

Type: Coding

Length:33bp

Description/Usage: This part codes the expression of the TAT-PTD that is used in helping the plasmid transfect into the eukaryocyte.

Histone H4

ID: BBa_K1439006

Type: Coding

Length:312bp

Description/Usage: This part codes the expression of the Histone H4 that is used in combing and protecting the plasmid in bacterium.

CMV promoter

ID: BBa_K1439007

Type: Regulatory

Length:588bp

Description/Usage: We use CMV to improve the expression of TAT:H4 and the efficiency of transfection.

TAT-H4-B0015

ID: BBa_K1439008

Type: Composite

Length:530bp

Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte. It has a terminator and can be added different promoters.

Lysis device induced by L-arabinose

ID: BBa_K1439009

Type: Device

Length:1923bp

Description/Usage:This part encodes the expression of the lysis protein that is induced by L-arabinose mobility.

Lysis device induced by aTc

ID: BBa_K1439010

Type: Device

Length:2738bp

Description/Usage:This is the lysis device using three genes from enterobacteria phage T4: lysozyme, holin, and antiholin.Tet promoter can control the lysis of bacteria.

contact us: oucigem@163.com

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