Team:OUC-China/Judging

From 2014.igem.org

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       <p class="text-primary">Origin of transfer for the RP4-plasmid nic region</p>
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<style>
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       <p class="lead">ID: BBa_K1439000</p>  
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.divide { border-top: 1px solid #103081; }
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       <p class="lead">Type: conjugation</p>
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</style>
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       <p class="lead">Length:350bp</p>
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<h1>Biobrick</h1>
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       <p class="lead">Description/Usage: OriTRP4,the RP4 plasmid nic region,is where the relaxosome nicks the plasmid and conjugative transfer by RP4 plasmid machinery begins.</p>
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       <h4 class="text-primary divide">Origin of transfer for the RP4-plasmid nic region</h4>
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       <p class="lead"> </p>
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       <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439000">BBa_K1439000</a></p>  
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       <p class="text-primary">[OriTRP4]+[RFP]</p>
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       <p>Type: conjugation</p>
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       <p class="lead">ID: BBa_K1439001</p>
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       <p>Length:350bp</p>
-
       <p class="lead">Type: Composite</p>
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       <p>Description/Usage: OriTRP4,the RP4 plasmid nic region,is where the relaxosome nicks the plasmid and conjugative transfer by RP4 plasmid machinery begins.</p>
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       <p class="lead">Length:1427bp</p>
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       <p> </p>
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       <p class="lead">Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTRP4. Used to test plasmid mobility.</p>
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       <h4 class="text-primary divide">[OriTRP4]+[RFP]</h4>
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       <p class="lead"></p>
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       <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439001">BBa_K1439001</a></p>
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       <p class="text-primary">[OriTR]+[RFP]</p>
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       <p>Type: Composite</p>
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       <p class="lead">ID: BBa_K1439002</p>
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       <p>Length:1427bp</p>
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       <p class="lead">Type: Composite</p>
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       <p>Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTRP4. Used to test plasmid mobility.</p>
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       <p class="lead">Length:1448bp</p>
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       <p></p>
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       <p class="lead">Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.</p>
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       <h4 class="text-primary divide">[OriTR]+[RFP]</h4>
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       <p class="lead"></p>
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       <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439002">BBa_K1439002</a></p>
 +
       <p>Type: Composite</p>
 +
       <p>Length:1448bp</p>
 +
       <p>Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.</p>
 +
       <p></p>
       <p class="text-primary"> </p>
       <p class="text-primary"> </p>
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       <p class="lead">hEF1a_Cas9-VP16 MammoBlock</p>
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       <h4 class="text-primary divide">hEF1a_Cas9-VP16 MammoBlock</h4>
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       <p class="lead">ID: BBa_K1179003</p>
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       <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439003">BBa_K1179003</a></p>
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       <p class="lead">Type: Generator</p>
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       <p>Type: Generator</p>
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<p class="lead">Length: 5141bp</p>
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<p>Length: 5141bp</p>
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<p class="lead">Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with guide RNA binding sites upstream.</p>
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<p>Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with guide RNA binding sites upstream.</p>
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<p class="text-primary">TAT-H4</p>
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<h4 class="text-primary divide">TAT-H4</h4>
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<p class="lead">ID:BBa_K1439004</p>
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<p>ID: <a href="http://parts.igem.org/Part:BBa_K1439004">BBa_K1439004</a></p>
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<p class="lead">Type: Coding</p>
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<p>Type: Coding</p>
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<p class="lead">Length:393bp</p>
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<p>Length:393bp</p>
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<p class="lead">Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte.</p>
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<p>Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte.</p>
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<p class="text-primary">TAT-PTD</p>
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<h4 class="text-primary divide">TAT-PTD</h4>
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<p class="lead">ID:BBa_K1439005</p>
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<p>ID: <a href="http://parts.igem.org/Part:BBa_K1439005">BBa_K1439005</a></p>
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<p class="lead">Type: Coding</p>
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<p>Type: Coding</p>
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<p class="lead">Length:33bp</p>
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<p>Length:33bp</p>
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<p class="lead">Description/Usage: This part codes the expression of the TAT-PTD that is used in helping the plasmid transfect into the eukaryocyte.</p>
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<p>Description/Usage: This part codes the expression of the TAT-PTD that is used in helping the plasmid transfect into the eukaryocyte.</p>
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<p class="text-primary">Histone H4</p>
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<h4 class="text-primary divide">Histone H4</h4>
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<p class="lead">ID:BBa_K1439006</p>
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<p>ID: <a href="http://parts.igem.org/Part:BBa_K1439006">BBa_K1439006</a></p>
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<p class="lead">Type: Coding</p>
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<p>Type: Coding</p>
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<p class="lead">Length:312bp</p>
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<p>Length:312bp</p>
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<p class="lead">Description/Usage: This part codes the expression of the Histone H4 that is used in combing and protecting the plasmid in bacterium.</p>
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<p>Description/Usage: This part codes the expression of the Histone H4 that is used in combing and protecting the plasmid in bacterium.</p>
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<p class="text-primary">CMV promoter</p>
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<h4 class="text-primary divide">CMV promoter</h4>
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<p class="lead">ID:BBa_K1439007</p>
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<p>ID: <a href="http://parts.igem.org/Part:BBa_K1439007">BBa_K1439007</a></p>
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<p class="lead">Type: Regulatory</p>
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<p>Type: Regulatory</p>
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<p class="lead">Length:588bp</p>
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<p>Length:588bp</p>
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<p class="lead">Description/Usage: We use CMV to improve the expression of TAT:H4 and the efficiency of transfection.</p>
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<p>Description/Usage: We use CMV to improve the expression of TAT:H4 and the efficiency of transfection.</p>
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<p class="text-primary">TAT-H4-B0015</p>
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<h4 class="text-primary divide">TAT-H4-B0015</h4>
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<p class="lead">ID:BBa_K1439008</p>
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<p>ID: <a href="http://parts.igem.org/Part:BBa_K1439008">BBa_K1439008</a></p>
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<p class="lead">Type: Composite</p>
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<p>Type: Composite</p>
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<p class="lead">Length:530bp</p>
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<p>Length:530bp</p>
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<p class="lead">Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte. It has a terminator and can be added different promoters.</p>
+
<p>Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte. It has a terminator and can be added different promoters.</p>
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<p class="text-primary">Lysis device induced by L-arabinose</p>
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<h4 class="text-primary divide">Lysis device induced by L-arabinose</h4>
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       <p class="lead">ID: BBa_K1439009</p>
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       <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439009">BBa_K1439009</a></p>
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       <p class="lead">Type: Device</p>
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       <p>Type: Device</p>
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       <p class="lead">Length:1923bp</p>
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       <p>Length:1923bp</p>
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       <p class="lead">Description/Usage:This part encodes the expression of the lysis protein that is induced by L-arabinose mobility.</p>
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       <p>Description/Usage:This part encodes the expression of the lysis protein that is induced by L-arabinose mobility.</p>
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       <p class="lead"></p>
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       <p></p>
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       <p class="text-primary">Lysis device induced by aTc</p>
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       <h4 class="text-primary divide">Lysis device induced by aTc</h4>
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       <p class="lead">ID: BBa_K1439010</p>
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       <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439010">BBa_K1439010</a></p>
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       <p class="lead">Type: Device</p>
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       <p>Type: Device</p>
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       <p class="lead">Length:2738bp</p>
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       <p>Length:2738bp</p>
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       <p class="lead">Description/Usage:This is the lysis device using three genes from enterobacteria phage T4: lysozyme, holin, and antiholin.Tet promoter can control the lysis of bacteria.
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       <p>Description/Usage:This is the lysis device using three genes from enterobacteria phage T4: lysozyme, holin, and antiholin.Tet promoter can control the lysis of bacteria.
        
        
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    <img src="https://static.igem.org/mediawiki/2014/a/ad/OUC-China_Index_OrgLogo.png" />
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    <img src="https://static.igem.org/mediawiki/2014/e/ec/OUC-China_Index_OrgTitle.jpg" />
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    <img src="https://static.igem.org/mediawiki/2014/9/96/OUC-China_Foot_ouclogo.png" />
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    <div style="clear:both"></div>
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  </div>
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  <p>contact us: <a href="mailto:oucigem@163.com">oucigem@163.com</a></p>
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</div>
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Latest revision as of 03:07, 18 October 2014

Biobrick

Origin of transfer for the RP4-plasmid nic region

ID: BBa_K1439000

Type: conjugation

Length:350bp

Description/Usage: OriTRP4,the RP4 plasmid nic region,is where the relaxosome nicks the plasmid and conjugative transfer by RP4 plasmid machinery begins.

[OriTRP4]+[RFP]

ID: BBa_K1439001

Type: Composite

Length:1427bp

Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTRP4. Used to test plasmid mobility.

[OriTR]+[RFP]

ID: BBa_K1439002

Type: Composite

Length:1448bp

Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.

hEF1a_Cas9-VP16 MammoBlock

ID: BBa_K1179003

Type: Generator

Length: 5141bp

Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with guide RNA binding sites upstream.

TAT-H4

ID: BBa_K1439004

Type: Coding

Length:393bp

Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte.

TAT-PTD

ID: BBa_K1439005

Type: Coding

Length:33bp

Description/Usage: This part codes the expression of the TAT-PTD that is used in helping the plasmid transfect into the eukaryocyte.

Histone H4

ID: BBa_K1439006

Type: Coding

Length:312bp

Description/Usage: This part codes the expression of the Histone H4 that is used in combing and protecting the plasmid in bacterium.

CMV promoter

ID: BBa_K1439007

Type: Regulatory

Length:588bp

Description/Usage: We use CMV to improve the expression of TAT:H4 and the efficiency of transfection.

TAT-H4-B0015

ID: BBa_K1439008

Type: Composite

Length:530bp

Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte. It has a terminator and can be added different promoters.

Lysis device induced by L-arabinose

ID: BBa_K1439009

Type: Device

Length:1923bp

Description/Usage:This part encodes the expression of the lysis protein that is induced by L-arabinose mobility.

Lysis device induced by aTc

ID: BBa_K1439010

Type: Device

Length:2738bp

Description/Usage:This is the lysis device using three genes from enterobacteria phage T4: lysozyme, holin, and antiholin.Tet promoter can control the lysis of bacteria.

contact us: oucigem@163.com

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