Team:Cornell/notebook/protocols

From 2014.igem.org

(Difference between revisions)
 
(7 intermediate revisions not shown)
Line 13: Line 13:
</div>
</div>
</div>
</div>
-
<div class="container" id="top">
+
<div class="container">
<div class="row">
<div class="row">
-
<div class="thumbnail">
+
<div class="col-md-9 col-xs-12">
-
<img src="https://static.igem.org/mediawiki/2014/6/60/Cornell_IMG_4991_small.jpg">
+
<div class="thumbnail">
 +
<img src="https://static.igem.org/mediawiki/2014/6/60/Cornell_IMG_4991_small.jpg">
 +
</div>
</div>
</div>
-
            <div style="text-align:center">
+
<div class="col-md-3 col-xs-6" style="margin-top: 70px;">
-
<div class="col-md-4 col-xs-6">
+
<h3>DNA Purification</h3>
<h3>DNA Purification</h3>
-
<ul style="list-style-type:none">
+
<ul>
<li>
<li>
<a href="https://static.igem.org/mediawiki/2012/3/35/Ezna_Miniprep.pdf" target="_blank">EZNA Miniprep</a>
<a href="https://static.igem.org/mediawiki/2012/3/35/Ezna_Miniprep.pdf" target="_blank">EZNA Miniprep</a>
Line 31: Line 32:
<a href="https://static.igem.org/mediawiki/2012/2/2d/Qiagen_Miniprep.pdf" target="_blank">Qiagen Miniprep</a>
<a href="https://static.igem.org/mediawiki/2012/2/2d/Qiagen_Miniprep.pdf" target="_blank">Qiagen Miniprep</a>
</li>
</li>
-
<li>
+
<li>
<a href="https://static.igem.org/mediawiki/2013/7/79/Column_Purification.pdf" target="_blank">Column Purification</a>
<a href="https://static.igem.org/mediawiki/2013/7/79/Column_Purification.pdf" target="_blank">Column Purification</a>
</li>
</li>
</ul>
</ul>
-
</div>
+
<h3>PCR Methods</h3>
-
<div class="col-md-4 col-xs-6">
+
                <ul>
-
                                <h3>Plasmid Editing</h3>
+
                    <li>
-
                                      <ul style="list-style-type:none">
+
<a href="https://static.igem.org/mediawiki/2013/3/30/Q5_PCR.pdf" target="_blank">Q5 PCR</a>
-
<li>
+
-
<a href="https://static.igem.org/mediawiki/2012/a/af/Double_Digest.pdf" target="_blank">Double Digest</a>
+
</li>
</li>
<li>
<li>
-
<a href="https://static.igem.org/mediawiki/2012/f/fb/Single_Digest.pdf" target="_blank">Single Digest</a>
+
<a href="https://static.igem.org/mediawiki/2012/2/26/Phusion_PCR.pdf" target="_blank">Phusion PCR</a>
</li>
</li>
<li>
<li>
-
<a href="https://static.igem.org/mediawiki/2012/a/a5/Site_Directed_Mutagenesis.pdf" target="_blank">Site Directed Mutagenesis</a>
+
<a href="https://static.igem.org/mediawiki/2012/4/4b/Taq_PCR.pdf" target="_blank">Taq PCR</a>
</li>
</li>
-
 
-
                                        <li>
 
-
<a href="https://2014.igem.org/File:Vector_Dephosphorylation.pdf" target="_blank">Vector Dephosphorylation</a>
 
-
</li>
 
-
 
<li>
<li>
-
<a href="https://static.igem.org/mediawiki/2012/a/a7/T4_Ligase_Ligation.pdf" target="_blank">T4 Ligase Ligation</a>
+
<a href="https://static.igem.org/mediawiki/2012/3/33/Vent_PCR.pdf" target="_blank">Vent PCR</a>
</li>
</li>
-
                                  </ul>            
+
</ul>
 +
</div>
</div>
</div>
-
                        <div class="col-md-4 col-xs-6">
+
<div class="row">
-
                                  <h3>PCR Methods</h3>
+
<div class="col-md-3 col-xs-4">
-
                                        <ul style="list-style-type:none">
+
<h3>Metal and Growth Quantification</h3>
-
                                        <li>
+
<ul>
-
<a href="https://static.igem.org/mediawiki/2013/3/30/Q5_PCR.pdf" target="_blank">Q5 PCR</a>
+
-
</li>
+
<li>
<li>
-
<a href="https://static.igem.org/mediawiki/2012/2/26/Phusion_PCR.pdf" target="_blank">Phusion PCR</a>
+
<a href="https://static.igem.org/mediawiki/2014/f/f6/Cornell_Growth_Experiment_Protocol.pdf" target="_blank">Growth Experiments</a>
</li>
</li>
<li>
<li>
-
<a href="https://static.igem.org/mediawiki/2012/4/4b/Taq_PCR.pdf" target="_blank">Taq PCR</a>
+
<a href="https://static.igem.org/mediawiki/2014/e/e3/Cornell_Batch_Metal_Sequestration_Experiments.pdf" target="_blank">Batch Metal Sequestration Experiments</a>
</li>
</li>
-
<li>
+
                                        <li>
-
<a href="https://static.igem.org/mediawiki/2012/3/33/Vent_PCR.pdf" target="_blank">Vent PCR</a>
+
<a href="https://static.igem.org/mediawiki/2014/1/14/Cornell_Phen_Green_SK_Metal_Sequestration_Experiments.pdf" target="_blank">Kinetic Metal Sequestration Experiments</a>
</li>
</li>
-
</ul>
+
</ul>    
-
</div>
+
</div>
-
</div>
+
<div class="col-md-3 col-xs-4">
-
</div>
+
-
                  <div class="row">
+
-
                    <div style="text-align:center">
+
-
<div class="col-md-4 col-xs-6">
+
<h3>Plasmid Insertion</h3>
<h3>Plasmid Insertion</h3>
-
<ul style="list-style-type:none">
+
<ul>
-
                                      <li>
+
<li>
<a href="https://static.igem.org/mediawiki/2012/0/07/Ecoli_electroporation.pdf" target="_blank"><i>E.coli</i> Electroporation</a>
<a href="https://static.igem.org/mediawiki/2012/0/07/Ecoli_electroporation.pdf" target="_blank"><i>E.coli</i> Electroporation</a>
</li>
</li>
Line 94: Line 83:
<a href="https://static.igem.org/mediawiki/2014/9/99/Cornell_iGEM_Chemically_Competent_Stocks.pdf" target="_blank">Preparing Chemically Compotent <i>E.coli</i></a>
<a href="https://static.igem.org/mediawiki/2014/9/99/Cornell_iGEM_Chemically_Competent_Stocks.pdf" target="_blank">Preparing Chemically Compotent <i>E.coli</i></a>
</li>
</li>
-
                                  </ul>
+
                </ul>
-
</div>
+
</div>
-
                        <div class="col-md-4 col-xs-6">
+
<div class="col-md-3 col-xs-4">
-
                              <h3>DNA Assessment</h3>
+
<h3>DNA Assessment</h3>
-
                                  <ul style="list-style-type:none">
+
                <ul>
-
                                        <li>
+
                    <li>
<a href="https://static.igem.org/mediawiki/2012/6/6c/DNA_Gel_Electrophoresis.pdf" target="_blank">DNA Gel Electrophoresis</a>
<a href="https://static.igem.org/mediawiki/2012/6/6c/DNA_Gel_Electrophoresis.pdf" target="_blank">DNA Gel Electrophoresis</a>
</li>
</li>
Line 105: Line 94:
<a href="https://static.igem.org/mediawiki/2012/0/03/Sequencing_Preparation.pdf" target="_blank">Preparation of DNA for Sequencing</a>
<a href="https://static.igem.org/mediawiki/2012/0/03/Sequencing_Preparation.pdf" target="_blank">Preparation of DNA for Sequencing</a>
</li>
</li>
-
                                  </ul>
+
                </ul>
-
</div>
+
</div>
-
                        <div class="col-md-4 col-xs-6">
+
<div class="col-md-3 col-xs-4">
-
                            <h3>Metal and Growth Quantification</h3>
+
<h3>Plasmid Editing</h3>
-
                                  <ul style="list-style-type:none">
+
                <ul>
-
                                        <li>
+
<li>
-
<a href="https://static.igem.org/mediawiki/2014/f/f6/Cornell_Growth_Experiment_Protocol.pdf" target="_blank">Growth Experiments</a>
+
<a href="https://static.igem.org/mediawiki/2012/a/af/Double_Digest.pdf" target="_blank">Double Digest</a>
</li>
</li>
<li>
<li>
-
<a href="https://static.igem.org/mediawiki/2014/e/e3/Cornell_Batch_Metal_Sequestration_Experiments.pdf" target="_blank">Batch Metal Sequestration Experiments</a>
+
<a href="https://static.igem.org/mediawiki/2012/f/fb/Single_Digest.pdf" target="_blank">Single Digest</a>
</li>
</li>
-
                                        <li>
+
<li>
-
<a href="https://static.igem.org/mediawiki/2014/1/14/Cornell_Phen_Green_SK_Metal_Sequestration_Experiments.pdf" target="_blank">Kinetic Metal Sequestration Experiments</a>
+
<a href="https://static.igem.org/mediawiki/2012/a/a5/Site_Directed_Mutagenesis.pdf" target="_blank">Site Directed Mutagenesis</a>
</li>
</li>
-
                                    </ul>
+
 
-
</div>
+
                    <li>
-
</div>
+
<a href="https://static.igem.org/mediawiki/2014/e/ef/Vector_Dephosphorylation.pdf" target="_blank">Vector Dephosphorylation</a>
-
</div>
+
</li>
-
+
 
-
</div>
+
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/a/a7/T4_Ligase_Ligation.pdf" target="_blank">T4 Ligase Ligation</a>
 +
</li>
 +
                </ul>   
 +
</div>
 +
</div>
</div>
</div>
   </body>
   </body>
</html>
</html>

Latest revision as of 02:51, 18 October 2014

Cornell iGEM

web stats

Protocols

DNA Purification

PCR Methods

Metal and Growth Quantification

Plasmid Insertion

DNA Assessment

Plasmid Editing

Retrieved from "http://2014.igem.org/Team:Cornell/notebook/protocols"