Team:Cornell/notebook/protocols

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<h1 style="padding: 0px; margin-bottom: 0px;">Protocols</h1>
<h1 style="padding: 0px; margin-bottom: 0px;">Protocols</h1>
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Text text text Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Aenean commodo ligula eget dolor. Aenean massa. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Donec quam felis, ultricies nec, pellentesque eu, pretium quis, sem. Nulla consequat massa quis enim. Donec pede justo, fringilla vel, aliquet nec, vulputate eget, arcu. In enim justo, rhoncus ut, imperdiet a, venenatis vitae, justo. Nullam dictum felis eu pede mollis pretium. Integer tincidunt.
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Far far away, behind the word mountains, far from the countries Vokalia and Consonantia, there live the blind texts. Separated they live in Bookmarksgrove right at the coast of the Semantics, a large language ocean. A small river named Duden flows by their place and supplies it with the necessary regelialia.
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Far far away, behind the word mountains, far from the countries Vokalia and Consonantia, there live the blind texts. Separated they live in Bookmarksgrove right at the coast of the Semantics, a large language ocean. A small river named Duden flows by their place and supplies it with the necessary regelialia.  
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<h3>DNA Purification</h3>
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<a href="https://static.igem.org/mediawiki/2012/3/35/Ezna_Miniprep.pdf" target="_blank">EZNA Miniprep</a>
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<a href="https://static.igem.org/mediawiki/2012/2/21/Qiagen_Gel_Extraction.pdf" target="_blank">Qiagen Gel Extraction</a>
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<a href="https://static.igem.org/mediawiki/2012/2/2d/Qiagen_Miniprep.pdf" target="_blank">Qiagen Miniprep</a>
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<a href="https://static.igem.org/mediawiki/2013/7/79/Column_Purification.pdf" target="_blank">Column Purification</a>
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<h3>PCR Methods</h3>
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<a href="https://static.igem.org/mediawiki/2013/3/30/Q5_PCR.pdf" target="_blank">Q5 PCR</a>
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<a href="https://static.igem.org/mediawiki/2012/2/26/Phusion_PCR.pdf" target="_blank">Phusion PCR</a>
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<a href="https://static.igem.org/mediawiki/2012/4/4b/Taq_PCR.pdf" target="_blank">Taq PCR</a>
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<a href="https://static.igem.org/mediawiki/2012/3/33/Vent_PCR.pdf" target="_blank">Vent PCR</a>
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But I must explain to you how all this mistaken idea of denouncing pleasure and praising pain was born and I will give you a complete account of the system, and expound the actual teachings of the great explorer of the truth, the master-builder of human happiness.
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<h3>Metal and Growth Quantification</h3>
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<a href="https://static.igem.org/mediawiki/2014/f/f6/Cornell_Growth_Experiment_Protocol.pdf" target="_blank">Growth Experiments</a>
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<a href="https://static.igem.org/mediawiki/2014/e/e3/Cornell_Batch_Metal_Sequestration_Experiments.pdf" target="_blank">Batch Metal Sequestration Experiments</a>
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<a href="https://static.igem.org/mediawiki/2014/1/14/Cornell_Phen_Green_SK_Metal_Sequestration_Experiments.pdf" target="_blank">Kinetic Metal Sequestration Experiments</a>
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<h3>Plasmid Insertion</h3>
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The quick, brown fox jumps over a lazy dog. DJs flock by when MTV ax quiz prog. Junk MTV quiz graced by fox whelps. Bawds jog, flick quartz, vex nymphs. Waltz, bad nymph, for quick jigs vex! Fox nymphs grab quick-jived waltz. Brick quiz whangs jumpy veldt fox. Bright vixens jump; dozy fowl quack.
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<a href="https://static.igem.org/mediawiki/2012/0/07/Ecoli_electroporation.pdf" target="_blank"><i>E.coli</i> Electroporation</a>
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<a href="https://static.igem.org/mediawiki/2012/5/57/Making_Ecoli_Electrocompetent.pdf" target="_blank">Preparing Electrocompetent <i>E.coli</i></a>
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<a href="https://static.igem.org/mediawiki/2014/8/84/Cornell_iGEM_Heat_Shock.pdf" target="_blank"><i>E.coli</i> Heat Shock</a>
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<a href="https://static.igem.org/mediawiki/2014/9/99/Cornell_iGEM_Chemically_Competent_Stocks.pdf" target="_blank">Preparing Chemically Compotent <i>E.coli</i></a>
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<h3>DNA Assessment</h3>
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<a href="https://static.igem.org/mediawiki/2012/6/6c/DNA_Gel_Electrophoresis.pdf" target="_blank">DNA Gel Electrophoresis</a>
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<a href="https://static.igem.org/mediawiki/2012/0/03/Sequencing_Preparation.pdf" target="_blank">Preparation of DNA for Sequencing</a>
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<h3>Plasmid Editing</h3>
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<a href="https://static.igem.org/mediawiki/2012/a/af/Double_Digest.pdf" target="_blank">Double Digest</a>
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<a href="https://static.igem.org/mediawiki/2012/f/fb/Single_Digest.pdf" target="_blank">Single Digest</a>
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<a href="https://static.igem.org/mediawiki/2012/a/a5/Site_Directed_Mutagenesis.pdf" target="_blank">Site Directed Mutagenesis</a>
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<a href="https://static.igem.org/mediawiki/2014/e/ef/Vector_Dephosphorylation.pdf" target="_blank">Vector Dephosphorylation</a>
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<a href="https://static.igem.org/mediawiki/2012/a/a7/T4_Ligase_Ligation.pdf" target="_blank">T4 Ligase Ligation</a>
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No one rejects, dislikes, or avoids pleasure itself, because it is pleasure, but because those who do not know how to pursue pleasure rationally encounter consequences that are extremely painful. Nor again is there anyone who loves or pursues or desires to obtain pain of itself, because it is pain, but because occasionally circumstances occur in which toil and pain can procure him some great pleasure. To take a trivial example, which of us ever undertakes laborious physical exercise, except to obtain some advantage from it? But who has any right to find fault with a man who chooses to enjoy a pleasure that has no annoying consequences, or one who avoids a pain that produces no resultant pleasure? On the other hand, we denounce with righteous indignation and dislike men who are so beguiled and demoralized by the charms of pleasure of the moment, so blinded by desire, that they cannot foresee.
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The quick, brown fox jumps over a lazy dog. DJs flock by when MTV ax quiz prog. Junk MTV quiz graced by fox whelps. Bawds jog, flick quartz, ex nymphs. Waltz, bad nymph, for quick jigs vex! Fox nymphs grab quick-jived waltz. Brick quiz whangs jumpy veldt fox. Bright vixens jump; dozy fowl quack.
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<h1 style="margin-bottom: 0px">References</h1>
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Latest revision as of 02:51, 18 October 2014

Cornell iGEM

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Protocols

DNA Purification

PCR Methods

Metal and Growth Quantification

Plasmid Insertion

DNA Assessment

Plasmid Editing

Retrieved from "http://2014.igem.org/Team:Cornell/notebook/protocols"