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Team:HokkaidoU Japan/Notebook/H Stem/Replacement To 1A3
From 2014.igem.org
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<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li> | <li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li> | ||
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li> | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li> | ||
| - | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion# | + | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Evaluation">Evaluation</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
Revision as of 02:48, 18 October 2014
Notebook
Lab Documents
H-Stem Vector Exchange to pSB1A3
-
Start
-
Mini-Prep
R0010-stem-as-stem-B0015 (on pSB1A3) -
PCR
R0010-stem-as-stem-B0015(on pSB1C3) with 100up_F Tm:62.6℃ & 200dn_R Tm:66.6℃ -
Digestion
pSB1A3 with EcoRI & PstI, R0010-stem-as-stem-B0015 with EcoRI & PstI -
Ligation
0010-stem-as-stem-B0015 & pSB1A3 -
Transformation
R0010-stem-as-stem-B0015(on pSB1A3) 5.0µL to DH5alpha; -
Complete!
