"
Team:HokkaidoU Japan/Notebook/asB0034/140910 asB0034&B0034 GFP Assay
From 2014.igem.org
(Difference between revisions)
| Line 189: | Line 189: | ||
</div> | </div> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
| - | LB 2 mL for 9hours Turbidity was controlled 0.1 | + | LB 2 mL for 9hours |
| + | </div> | ||
| + | <div class="step-recipe"> | ||
| + | Turbidity was controlled 0.1 | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 02:45, 18 October 2014
Notebook
Lab Documents
140910 asB0034 & B0034 GFP Assay
-
Start
-
Double Transformation
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-B0040-B0015(on pSB6A1)each of 2.5µL DNA to DH5α -
Colony PCR
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(pSB6A1) pHN1257_up_F Tm:60.1℃ & asB0034-R Tm:68.7℃ -
Preculture
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-B0040-B0015(on pSB6A1)LB 2 mL for 9hoursTurbidity was controlled 0.1 -
Culture
M9ZB 2 mL IPTG 20 µ or notfor 9 hours, 11 hours, 13hours -
Assay
fluorescence imaging 488 nm 530 filter -
Complete!
