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Team:Purdue/Results/Wetlab Experiments
From 2014.igem.org
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'''Transformation of Bacillus Subtilis | '''Transformation of Bacillus Subtilis | ||
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| - | <p>We first conducted growth curve assays of our two strains (3A37 and 1A436) of Bacillus Subtilis. </p> | + | <p>We first conducted growth curve assays of our two strains (3A37 and 1A436) of Bacillus Subtilis obtained from The Ohio State Bacillus Stock Center . </p> |
<html><div z-index:100><img src="https://static.igem.org/mediawiki/2014/9/94/GrowthCurve2.png" width="550" height="350" align="center"></div></html> | <html><div z-index:100><img src="https://static.igem.org/mediawiki/2014/9/94/GrowthCurve2.png" width="550" height="350" align="center"></div></html> | ||
Revision as of 02:32, 18 October 2014
Our two main wet lab projects were the transformation of Bacillus Subtilis and assembly of our plasmids using Gibson Assembly. Transformation of Bacillus Subtilis
We first conducted growth curve assays of our two strains (3A37 and 1A436) of Bacillus Subtilis obtained from The Ohio State Bacillus Stock Center . Pérez-Miranda, S., Cabirol, N., George-Téllez, R., Zamudio-Rivera, L. S., & Fernández, F. J. (2007). O-CAS, a fast and universal method for siderophore detection. Journal of microbiological methods, 70(1), 127-131.
We synthesized our two plasmids using Integrated DNA Technologies (IDT). We currently have them as gBlocks and are trouble shooting the Gibson Assembly.Wet Lab Experiments
Results
Once we have functional circuits we will conduct the following Pytosidderophore Assay adapted from:
Gibson Assembly
