Team:LIKA-CESAR-Brasil/TestColialert

From 2014.igem.org

(Difference between revisions)
(Created page with "{{CSS/Main}} <html lang="pt-br"> <head> <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="viewport" content="width=device-wid...")
 
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<link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.2.0/css/bootstrap.min.css">
<link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.2.0/css/bootstrap.min.css">
-
     <link href="https://dl.dropbox.com/s/3g6ibzuen1skq16/lika.css" rel="stylesheet">
+
     <style type="text/css">
 +
body {
 +
font-size:12px;
 +
color:#111;
 +
height:100%;
 +
background:#ededed;
 +
/*min-width: 1326px;*/
 +
}
 +
.container {
 +
width:80%;
 +
background: rgb(255,255,255); /* Old browsers */
 +
background: -moz-linear-gradient(top,  rgba(255,255,255,1) 0%, rgba(237,237,237,1) 100%); /* FF3.6+ */
 +
background: -webkit-gradient(linear, left top, left bottom, color-stop(0%,rgba(255,255,255,1)), color-stop(100%,rgba(237,237,237,1))); /* Chrome,Safari4+ */
 +
background: -webkit-linear-gradient(top,  rgba(255,255,255,1) 0%,rgba(237,237,237,1) 100%); /* Chrome10+,Safari5.1+ */
 +
background: -o-linear-gradient(top,  rgba(255,255,255,1) 0%,rgba(237,237,237,1) 100%); /* Opera 11.10+ */
 +
background: -ms-linear-gradient(top,  rgba(255,255,255,1) 0%,rgba(237,237,237,1) 100%); /* IE10+ */
 +
background: linear-gradient(to bottom,  rgba(255,255,255,1) 0%,rgba(237,237,237,1) 100%); /* W3C */
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filter: progid:DXImageTransform.Microsoft.gradient( startColorstr='#ffffff', endColorstr='#ededed',GradientType=0 ); /* IE6-9 */
 +
}
 +
 
 +
.navbar-brand {
 +
padding: 5px;
 +
}
 +
.col-md-10  {
 +
width:100%;
 +
padding: 20px 70px 20px 50px;
 +
min-height:200px;
 +
padding-bottom:50px;
 +
}
 +
.col-md-4 {
 +
padding-bottom:70px;
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}
 +
.navbar-inverse {
 +
    background-color: #ededed;
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border-color: #ededed;
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}
 +
.navbar-inverse .navbar-nav > .active > a, .navbar-inverse .navbar-nav > .active > a:hover, .navbar-inverse .navbar-nav > .active > a:focus {
 +
    color: #006600;
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    background-color: #8cff00;
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}
 +
.navbar-inverse .navbar-nav > .open > a, .navbar-inverse .navbar-nav > .open > a:hover, .navbar-inverse .navbar-nav > .open > a:focus {
 +
    color: #006600;
 +
    background-color: #8cff00;
 +
}
 +
.navbar-inverse .navbar-nav > li > a:hover, .navbar-inverse .navbar-nav > li > a:focus {
 +
    color: #006600;
 +
    background-color: transparent;
 +
}
 +
.navbar-inverse .navbar-nav > li > a:focus {
 +
    color: #006600;
 +
    background-color: transparent;
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}
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.nav > li > a {
 +
    display: block;
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    padding: 25px 15px;
 +
    position: relative;
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}
 +
.menu{
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color:black;
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z-index:999;
 +
position:relative;
 +
float:right;
 +
margin:auto;
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}
 +
 
 +
.videohome{
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background:#ededed;
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}
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.menu ul{
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margin:0 auto;
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}
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.jumbotron {
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background:url("https://static.igem.org/mediawiki/2014/7/7a/Banner_top.jpg");
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border-top: solid 5px #8cff00;
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border-bottom: solid 5px #8cff00;
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background-size:cover;
 +
width:80%;
 +
height:331px;
 +
margin:auto;
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margin-top:40px;
 +
}
 +
.jumbotron02 {
 +
background:url("https://static.igem.org/mediawiki/2014/5/52/Banner_top02.jpg");
 +
border-top: solid 5px #8cff00;
 +
border-bottom: solid 5px #8cff00;
 +
background-size:cover;
 +
width:80%;
 +
height:331px;
 +
margin:auto;
 +
margin-top:80px;
 +
}
 +
.jumbotronSafety {
 +
background:url("https://static.igem.org/mediawiki/2014/7/7a/Banner_top.jpg");
 +
border-top: solid 5px #8cff00;
 +
border-bottom: solid 5px #8cff00;
 +
background-size:cover;
 +
width:80%;
 +
height:331px;
 +
margin:auto;
 +
margin-top:80px;
 +
}
 +
.jumbotronParts {
 +
background:url("https://static.igem.org/mediawiki/2014/b/be/Banner_top04.jpg");
 +
border-top: solid 5px #8cff00;
 +
border-bottom: solid 5px #8cff00;
 +
background-size:cover;
 +
width:80%;
 +
height:331px;
 +
margin:auto;
 +
margin-top:80px;
 +
}
 +
.jumbotronHuman {
 +
background:url("https://static.igem.org/mediawiki/2014/1/12/Banner_top05.jpg");
 +
border-top: solid 5px #8cff00;
 +
border-bottom: solid 5px #8cff00;
 +
background-size:cover;
 +
width:80%;
 +
height:331px;
 +
margin:auto;
 +
margin-top:80px;
 +
}
 +
.jumbotronProject {
 +
background:url("https://static.igem.org/mediawiki/2014/b/be/Banner_top04.jpg");
 +
border-top: solid 5px #8cff00;
 +
border-bottom: solid 5px #8cff00;
 +
background-size:cover;
 +
width:80%;
 +
height:331px;
 +
margin:auto;
 +
margin-top:80px;
 +
}
 +
.jumbotronAdvisors {
 +
background:url("https://static.igem.org/mediawiki/2014/3/3b/Banner_top03.jpg");
 +
border-top: solid 5px #8cff00;
 +
border-bottom: solid 5px #8cff00;
 +
background-size:cover;
 +
width:80%;
 +
height:331px;
 +
margin:auto;
 +
margin-top:80px;
 +
}
 +
 
 +
.igem-logo{
 +
    background:url("https://static.igem.org/mediawiki/2014/4/4e/Banner_top_03.png") ;
 +
    background-color:transparent;
 +
    width: 50px;
 +
    height:50px;
 +
    z-index: 99;
 +
    background-repeat: no-repeat;
 +
}
 +
 
 +
/*
 +
.navbar .container {
 +
min-width: 1322px;
 +
    z-index: 9999;
 +
}
 +
*/
 +
 
 +
h2 {
 +
font-size: 26px;
 +
color:#339900;
 +
}
 +
.videocapa{
 +
width:35%;
 +
float:right;
 +
min-height:300px;
 +
margin-top:10px;
 +
}
 +
p {
 +
font-size: 12px;
 +
color:#444;
 +
}
 +
footer {
 +
background:#b8b8b8;
 +
width:100%;
 +
padding: 50px;
 +
  clear:both;
 +
position: relative;
 +
bottom:0;
 +
border-top: solid 8px #8cff00;
 +
}
 +
footer p {
 +
margin:auto;
 +
padding-top:10px;
 +
text-align:center;
 +
width:50%;
 +
color:#006600;
 +
}
 +
.firstHeading {
 +
display:none;
 +
}
 +
table{
 +
color:#888;
 +
font-size:16px;
 +
border: solid 1px #ccc;
 +
}
 +
h1 {
 +
font-size: 26px;
 +
color:#339900;
 +
}
 +
h2 {
 +
font-size: 22px;
 +
color:#339900;
 +
}
 +
h3 {
 +
font-size: 18px;
 +
color:#339900;
 +
}
 +
h4 {
 +
font-size: 16px;
 +
color:#339900;
 +
}
 +
 
 +
.listHorizontal{
 +
    list-style: none;
 +
    display:inline-block;
 +
    margin-left: 0.3em;
 +
}
 +
 
 +
.listHorizontal li{
 +
    padding: 0px 5px 0px 5px;
 +
}
 +
 
 +
div#globalWrapper {
 +
position: relative;
 +
font-size: 100%;
 +
width: 100%;
 +
margin: 0;
 +
padding: 0;
 +
padding-bottom: 10px;
 +
}
 +
 
 +
</style>
   </head>
   </head>
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         <li><a href="https://2014.igem.org/Team:LIKA-CESAR-Brasil/Safety">SAFETY</a></li>
         <li><a href="https://2014.igem.org/Team:LIKA-CESAR-Brasil/Safety">SAFETY</a></li>
         <li><a href="https://2014.igem.org/Team:LIKA-CESAR-Brasil/Atribuitons">ATRIBUITIONS</a></li>
         <li><a href="https://2014.igem.org/Team:LIKA-CESAR-Brasil/Atribuitons">ATRIBUITIONS</a></li>
 +
        <li class="igem-logo"><a href="https://2014.igem.org/"></a></li>
             </ul>
             </ul>
           </div><!--/.nav-collapse -->
           </div><!--/.nav-collapse -->

Latest revision as of 02:15, 18 October 2014

LIKA | CESAR

NOTEBOOK

Test of ColiAlert with different pH

  • 1) 200 µl of the bacterial suspension (cells with chromogenic protein) were mixed with 1 mL of acetic acid. Homogenized mixture to the tube changing color. After change of color was added to this tube 500 µl a solution of tri-basic. Mix until the solution color change. (Reversible by changing pH reaction).
  • 2) 200 µl of the bacterial suspension was mixed with 1 ml of lysis buffer. The solution was homogenized by observing color change. After change of color was added to 500 µl Tris base solution, homogenized again for observation of solution color change. (Reaction irreversible cell bacterial lysis and release intracellular proteins).
Marca LIKA Marca CESAR

Retrieved from "http://2014.igem.org/Team:LIKA-CESAR-Brasil/TestColialert"