Team:UI-Indonesia

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<a href="https://2013.igem.org/Team:Peking">Home</a>
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<h2>About Our Project</h2>
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<p>Biofilm is a matrix extracellular polymeric substances (EPS) that surrounds microbial colonies and causes more than 65% microbial infections. Most of the pathogenic bacteria could make biofilm that increases their antibiotic resistance and increase its pathogenecity. Our <i>Escherichia coli</i> can degrade biofilm from various bacteria after it seeks them by detecting their quorum sensing signal molecule. Our team focuses in <i>Vibrio cholerae</i> because it causes cholera, one of the deadly tropical country disease. We present our <i>E. coli</i> that can degrade biofilm of <i>V. cholerae</i> after it detects quorum sensing signal molecule from <i>V. cholerae</i>, CAI-1, by CqsS receptor. Then our <i>E. coli</i> will activate motility gene, CheZ, to hunt <i>V. cholerae</i>. When it arrives, our bacteria will degrade the biofilm by secreting enzymes, such as α-amylase, nuclease, and substilisin to break down the matrix and finally secretes peptide 1018 that will kill <i>V. cholerae</i>.
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Our project can be applied in broad application. Not only fighting the <i>Vibrio cholerae</i> but also the other bacteria such as: <i>Pseudomonas aerugoinosa</i>, <i>Bacillus substillis</i>, <i>Klebsiella pneumonia</i>, <i>Staphylococcus aureus</i>, and <i>E. coli</i> Top 10.</p>
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<li><a href="https://2013.igem.org/Team:Peking/Team/Members">Members</a></li>
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<li><a href="https://2013.igem.org/Team:Peking/Team/Notebook">Notebook</a></li>
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<a href="https://2013.igem.org/Team:Peking/Project">Project</a>
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                                <li><a href="https://2013.igem.org/Team:Peking/Project/SensorMining">Biosensor Mining</a></li>
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<li><a href="https://2013.igem.org/Team:Peking/Project/BioSensors">Biosensors</a></li>
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<li><a href="https://2013.igem.org/Team:Peking/DataPage/JudgingCriteria">Judging Criteria</a></li>
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<h1 id="ProjectName">Aromatics Scouts<img id="LittleScout" src="https://static.igem.org/mediawiki/igem.org/8/8a/Peking2013_home_Telescope.png" /></h1>
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                <h1 id="ProjectSubname">A Comprehensive Biosensor Toolkit for Profiling Aromatic Compounds in the Environment</h1>
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<B>★Presentation:</B> Room 34-101, Saturday 12:30 PM, Session 2
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      &ensp;&ensp;&ensp;&ensp;<B>★Poster:</B> Sunday, #13 (Stata)
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              <p id="AbstractContent">Monitoring aromatic compounds in the environment remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed a functionally comprehensive biosensor toolkit to profile aromatics in the environment.
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Transcriptional regulators that each detect a specific class of aromatics were first bioinformatically determined; and then utilized to build a comprehensive set of biosensor circuits. Characterization on the detection profiles of individual biosensors and the orthogonality/crosstalk between them proved that these biosensors are very capable at profiling aromatics present in water.
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Moreover, for the ease of practical applications, two types of genetic devices were also developed as plug-ins for biosensors: "Adaptors", a set of conceptually novel devices to convert undetectable compounds into detectable compounds, and "Band-pass Filter", a "concentration filter" that allows the detection of analyte concentration within a specific range.
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We expect that these novel biosensors, together with the plug-in devices, will serve as intriguing synthetic biological tools for diverse practical applications.
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              <h1 id="Appendix1Title"><i>Mining aromatics-sensing Biobricks <br/>from the genomic database</i></h1>
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                    <h1 class="AppendixHeadLine">Biosensor Mining</h1>
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                    <p>The core component of our biosensor toolkit is the transcriptional regulators that sense aromatics. For the comprehensiveness of aromatics-sensing, a data-mining process was conducted to mine transcriptional regulators for each typical class of aromatics from the database Uniprot. </p>
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                    <a href="https://2013.igem.org/Team:Peking/Project/SensorMining">LEARN MORE</a>
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              <h1 id="Appendix2Title"><i>High-performance, profile-<br/>specific biosensors</i> </h1>
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                    <h1 class="AppendixHeadLine">Biosensors</h1>
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                    <p>A comprehensive set of biosensor circuits have been implemented using the aromatics-sensing transcriptional regulators. Each biosensor has a specific aromatics-sensing profile. Furthermore, the orthogonality of their sensing profiles was carefully assessed for practical applications.</p>
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                    <a href="https://2013.igem.org/Team:Peking/Project/BioSensors">LEARN MORE</a>
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              <h1 id="Appendix3Title"><i>Converting the undetectable into<br/>the detectable</i></h1>
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                    <h1 class="AppendixHeadLine">Adaptors</h1>
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                    <p> To expand the detection profiles of some biosensors, aromatics-metabolizing enzymes were taken from natural metabolic pathways, working as Adaptors to convert undetectable chemicals into detectable aromatics when coupled with biosensor circuits.</p>
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                    <a href="https://2013.igem.org/Team:Peking/Project/Plugins">LEARN MORE</a>
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              <h1 id="Appendix4Title"><i>Rapidly determining <br/> the analyte concentration</i></h1>
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                    <h1 class="AppendixHeadLine">Band-pass Filter</h1>
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                    <p>For the ease of practical analysis, a genetic device called "Band-pass Filter" has been constructed to allow the detection of analyte concentration within a specific range. Biosensors equipped with the Band-pass Filter can robustly quantify the aromatics in environmental samples. </p>
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                    <a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">LEARN MORE</a>
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              <h1 id="Appendix5Title"><i>Communications, Questionnaire survey,  <br/>Factory Visit</i> and interview</h1>
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                    <h1 class="AppendixHeadLine">Human Practice</h1>
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                    <p>To obtain the information about public awareness and the situation of aromatics pollution, a survey including factory visit and questionnaires has been conducted. We also guided an iGEM HS team and held "Model iGEM" as a competition without competitiveness.</p>
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                    <a href="https://2013.igem.org/Team:Peking/HumanPractice">LEARN MORE</a>
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Latest revision as of 01:54, 18 October 2014

About Our Project

Biofilm is a matrix extracellular polymeric substances (EPS) that surrounds microbial colonies and causes more than 65% microbial infections. Most of the pathogenic bacteria could make biofilm that increases their antibiotic resistance and increase its pathogenecity. Our Escherichia coli can degrade biofilm from various bacteria after it seeks them by detecting their quorum sensing signal molecule. Our team focuses in Vibrio cholerae because it causes cholera, one of the deadly tropical country disease. We present our E. coli that can degrade biofilm of V. cholerae after it detects quorum sensing signal molecule from V. cholerae, CAI-1, by CqsS receptor. Then our E. coli will activate motility gene, CheZ, to hunt V. cholerae. When it arrives, our bacteria will degrade the biofilm by secreting enzymes, such as α-amylase, nuclease, and substilisin to break down the matrix and finally secretes peptide 1018 that will kill V. cholerae.

Our project can be applied in broad application. Not only fighting the Vibrio cholerae but also the other bacteria such as: Pseudomonas aerugoinosa, Bacillus substillis, Klebsiella pneumonia, Staphylococcus aureus, and E. coli Top 10.

Retrieved from "http://2014.igem.org/Team:UI-Indonesia"