Team:UI-Indonesia

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    <h2 class="onBlack">Doesn't rubber come from trees?</h2>
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Let your eyes (and mouse) wander to these trees to discover our ideas on how to help the environment and change the future of rubber production. Take a look at our short <strong>project description</strong> below.
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The growing demand for natural rubber causes deforestation of the rainforest and occupation of arable lands, due to the establishment of new plantations. If producing rubber by bacteria succeeds, production of natural rubber will not be limited to the regions where the rubber tree can grow. Rather, rubber can be produced even in barren lands.  
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Our project aims to enable a common bacteria to produce natural rubber while grown under controlled conditions.
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Natural rubber is composed of molecules consisting of the substance IPP linked together like a chain. The common bacteria that we use (E. coli) already possesses the ability to produce the IPP, but it lacks the enzyme to connect the IPP links together into a chain.
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We introduced the enzyme that the rubber tree has for connecting the links into the bacteria. Furthermore, we introduced genes that allow the bacteria further production of the IPP links.  
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    <h2 class="onBlack">No, rubber is made in the lab.</h2>
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        <li><img src="https://static.igem.org/mediawiki/2014/1/15/UI-Indonesia_Image_SliderHP.jpg" /></li>
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If you wish to see how, click here to start the interactive tour.
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Just days before the deadline of iGEM 2013, we got the peaks that our entire summer built towards: Two small bumps on the NMR indicating the presence of bacterially produced rubber in our strain of E. coli. With a mad scramble to the finish line, our initial indication was reinforced. Click anywhere along this text to start the interactive tour, which will guide you along the path to rubber.
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<h2>About Our Project</h2>
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<p>Biofilm is a matrix extracellular polymeric substances (EPS) that surrounds microbial colonies and causes more than 65% microbial infections. Most of the pathogenic bacteria could make biofilm that increases their antibiotic resistance and increase its pathogenecity. Our <i>Escherichia coli</i> can degrade biofilm from various bacteria after it seeks them by detecting their quorum sensing signal molecule. Our team focuses in <i>Vibrio cholerae</i> because it causes cholera, one of the deadly tropical country disease. We present our <i>E. coli</i> that can degrade biofilm of <i>V. cholerae</i> after it detects quorum sensing signal molecule from <i>V. cholerae</i>, CAI-1, by CqsS receptor. Then our <i>E. coli</i> will activate motility gene, CheZ, to hunt <i>V. cholerae</i>. When it arrives, our bacteria will degrade the biofilm by secreting enzymes, such as α-amylase, nuclease, and substilisin to break down the matrix and finally secretes peptide 1018 that will kill <i>V. cholerae</i>.
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Our project can be applied in broad application. Not only fighting the <i>Vibrio cholerae</i> but also the other bacteria such as: <i>Pseudomonas aerugoinosa</i>, <i>Bacillus substillis</i>, <i>Klebsiella pneumonia</i>, <i>Staphylococcus aureus</i>, and <i>E. coli</i> Top 10.</p>
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Latest revision as of 01:54, 18 October 2014

About Our Project

Biofilm is a matrix extracellular polymeric substances (EPS) that surrounds microbial colonies and causes more than 65% microbial infections. Most of the pathogenic bacteria could make biofilm that increases their antibiotic resistance and increase its pathogenecity. Our Escherichia coli can degrade biofilm from various bacteria after it seeks them by detecting their quorum sensing signal molecule. Our team focuses in Vibrio cholerae because it causes cholera, one of the deadly tropical country disease. We present our E. coli that can degrade biofilm of V. cholerae after it detects quorum sensing signal molecule from V. cholerae, CAI-1, by CqsS receptor. Then our E. coli will activate motility gene, CheZ, to hunt V. cholerae. When it arrives, our bacteria will degrade the biofilm by secreting enzymes, such as α-amylase, nuclease, and substilisin to break down the matrix and finally secretes peptide 1018 that will kill V. cholerae.

Our project can be applied in broad application. Not only fighting the Vibrio cholerae but also the other bacteria such as: Pseudomonas aerugoinosa, Bacillus substillis, Klebsiella pneumonia, Staphylococcus aureus, and E. coli Top 10.

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