Team:EPF Lausanne/Parts

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<div id="parts">
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<h1 class="cntr">PARTS</h1>
<h1 class="cntr">PARTS</h1>
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<br/>
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<section id="dna">
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<h2 class="section-heading">Parts submitted by the 2014 EPFL iGEM team</h2>
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<h3 class="section-heading">DNA parts submitted by the 2014 EPFL iGEM team</h3>
<p class="lead">
<p class="lead">
-
Our team submitted a total of 43 Biobricks.
+
Our team submitted a total of 55 Biobricks (part 51 does not exist).</p>
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<p class="lead">
 +
In addition, 4 microfluidic designs have also been submitted to the registry.</p>
<table class="table table-striped table-hover" id="biobricks_list">
<table class="table table-striped table-hover" id="biobricks_list">
   <tr>
   <tr>
Line 95: Line 146:
     <th>Function</th>
     <th>Function</th>
     <th>Why do we use it?</th>
     <th>Why do we use it?</th>
 +
    <th>Group</th>
   </tr>
   </tr>
   <tr>
   <tr>
Line 101: Line 153:
     <td>Transcription factor</td>
     <td>Transcription factor</td>
     <td>To make most of our biobricks!</td>
     <td>To make most of our biobricks!</td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486001</td>
     <td class="biobrick_name">BBa_K1486001</td>
-
     <td>CpxR under arabinose promoter</td>
+
     <td>Arabinose promoter + CpxR</td>
-
     <td>Treanscription factor</td>
+
     <td>Transcription factor</td>
-
     <td></td>
+
     <td> </td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486002</td>
     <td class="biobrick_name">BBa_K1486002</td>
-
     <td>PAra + sfGFP CpxR</td>
+
     <td> Arabinose promoter + sfGFP-CpxR [Nterm]</td>
     <td>Expresses fused protein</td>
     <td>Expresses fused protein</td>
     <td>Test CpxR expression & Ara promoter</td>
     <td>Test CpxR expression & Ara promoter</td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486003</td>
     <td class="biobrick_name">BBa_K1486003</td>
-
     <td>Flexible linker</td>
+
     <td>Flexible linker 2x (GGGS)</td>
     <td>Attaches two proteins together</td>
     <td>Attaches two proteins together</td>
-
     <td></td>
+
     <td> </td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486004</td>
     <td class="biobrick_name">BBa_K1486004</td>
-
     <td>Flexible linker</td>
+
     <td>Flexible linker 2x (GGGGS)</td>
     <td>Attaches two proteins together</td>
     <td>Attaches two proteins together</td>
-
     <td></td>
+
     <td> </td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486005</td>
     <td class="biobrick_name">BBa_K1486005</td>
-
     <td>PAra + CpxR sfGFP</td>
+
     <td>Arabinose promoter + sfGFP-CpxR [Cterm]</td>
     <td>Expresses fused protein</td>
     <td>Expresses fused protein</td>
     <td>Test CpxR expression & Ara promoter</td>
     <td>Test CpxR expression & Ara promoter</td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
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     <td>IFP[1]</td>
     <td>IFP[1]</td>
     <td>N terminus of split IFP</td>
     <td>N terminus of split IFP</td>
-
     <td></td>
+
     <td> </td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
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     <td>IFP[2]</td>
     <td>IFP[2]</td>
     <td>C terminus of split IFP</td>
     <td>C terminus of split IFP</td>
-
     <td></td>
+
     <td> </td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486008</td>
     <td class="biobrick_name">BBa_K1486008</td>
-
     <td>CxpR & Split IFP1.4 [Cterm + Cterm]</td>
+
     <td>CpxR & Split IFP1.4 [Cterm + Cterm]</td>
     <td>Two CpxR CDS, each C terminus attached to a moiety of IFP</td>
     <td>Two CpxR CDS, each C terminus attached to a moiety of IFP</td>
     <td>Characterize CpxR dimerization</td>
     <td>Characterize CpxR dimerization</td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486009</td>
     <td class="biobrick_name">BBa_K1486009</td>
-
     <td>CxpR & Split IFP1.4 [Nterm + Nterm]</td>
+
     <td>CpxR & Split IFP1.4 [Nterm + Nterm]</td>
     <td>Two CpxR CDS, each N terminus attached to a moiety of IFP</td>
     <td>Two CpxR CDS, each N terminus attached to a moiety of IFP</td>
     <td>Characterize CpxR dimerization</td>
     <td>Characterize CpxR dimerization</td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486010</td>
     <td class="biobrick_name">BBa_K1486010</td>
-
     <td>CxpR & Split IFP1.4 [Nterm + Cterm]</td>
+
     <td>CpxR & Split IFP1.4 [Nterm + Cterm]</td>
     <td>Two CpxR CDS, each attached to a moiety of IFP</td>
     <td>Two CpxR CDS, each attached to a moiety of IFP</td>
     <td>Characterize CpxR dimerization</td>
     <td>Characterize CpxR dimerization</td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486011</td>
     <td class="biobrick_name">BBa_K1486011</td>
-
     <td>CxpR & Split IFP1.4 [Cterm + Nterm]</td>
+
     <td>CpxR & Split IFP1.4 [Cterm + Nterm]</td>
     <td>Two CpxR CDS, each attached to a moiety of IFP</td>
     <td>Two CpxR CDS, each attached to a moiety of IFP</td>
     <td>Characterize CpxR dimerization</td>
     <td>Characterize CpxR dimerization</td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486012</td>
     <td class="biobrick_name">BBa_K1486012</td>
-
     <td>CpxR + Nterm IFP</td>
+
     <td>CpxR-IFP[1] under pBAD</td>
     <td>CpxR with the Nterm moiety of IFP attached at its C terminus</td>
     <td>CpxR with the Nterm moiety of IFP attached at its C terminus</td>
     <td>Intermediate & control</td>
     <td>Intermediate & control</td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486013</td>
     <td class="biobrick_name">BBa_K1486013</td>
-
     <td>CpxR + Cterm IFP</td>
+
     <td>CpxR-IFP[2] under pBAD</td>
     <td>CpxR with the Cterm moiety of IFP attached at its C terminus</td>
     <td>CpxR with the Cterm moiety of IFP attached at its C terminus</td>
     <td>Intermediate & control</td>
     <td>Intermediate & control</td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486014</td>
     <td class="biobrick_name">BBa_K1486014</td>
-
     <td>Nterm IFP + CpxR</td>
+
     <td>IFP[1]-CpxR under pBAD</td>
     <td>CpxR with the Nterm moiety of IFP attached at its N terminus</td>
     <td>CpxR with the Nterm moiety of IFP attached at its N terminus</td>
     <td>Intermediate & control</td>
     <td>Intermediate & control</td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486015</td>
     <td class="biobrick_name">BBa_K1486015</td>
-
     <td>Cterm IFP + CpxR</td>
+
     <td>IFP[2]-CpxR under pBAD</td>
     <td>CpxR with the Cterm moiety of IFP attached at its N terminus</td>
     <td>CpxR with the Cterm moiety of IFP attached at its N terminus</td>
     <td>Intermediate & control</td>
     <td>Intermediate & control</td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486016</td>
     <td class="biobrick_name">BBa_K1486016</td>
-
     <td>Nterm fLuc</td>
+
     <td>fLuc[1]</td>
     <td>N terminus moiety of the firefly luciferase</td>
     <td>N terminus moiety of the firefly luciferase</td>
-
     <td></td>
+
     <td> </td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486017</td>
     <td class="biobrick_name">BBa_K1486017</td>
-
     <td>Cterm fLuc</td>
+
     <td>fLuc[2]</td>
     <td>C terminus moiety of the firefly luciferase</td>
     <td>C terminus moiety of the firefly luciferase</td>
-
     <td></td>
+
     <td> </td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486018</td>
     <td class="biobrick_name">BBa_K1486018</td>
-
     <td>PAra + Nterm fLuc + Cterm fLuc</td>
+
     <td>Arabinose promoter + fLuc[1] + fLuc[2]</td>
     <td>Split firefly luciferase under arabinose promoter</td>
     <td>Split firefly luciferase under arabinose promoter</td>
     <td>Control</td>
     <td>Control</td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486019</td>
     <td class="biobrick_name">BBa_K1486019</td>
-
     <td>Nterm rLuc</td>
+
     <td>rLuc[1]</td>
     <td>C terminus moiety of the renilla luciferase</td>
     <td>C terminus moiety of the renilla luciferase</td>
-
     <td></td>
+
     <td> </td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486020</td>
     <td class="biobrick_name">BBa_K1486020</td>
-
     <td>Cterm rLuc</td>
+
     <td>rLuc[2]</td>
     <td>N terminus moiety of the renilla luciferase</td>
     <td>N terminus moiety of the renilla luciferase</td>
-
     <td></td>
+
     <td> </td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486021</td>
     <td class="biobrick_name">BBa_K1486021</td>
-
     <td>PAra + Nterm rLuc + Cterm rLuc</td>
+
     <td>Arabinose promoter + rLuc[1] + rLuc[2]</td>
     <td>Split renilla luciferase under arabinose promoter</td>
     <td>Split renilla luciferase under arabinose promoter</td>
     <td>Control</td>
     <td>Control</td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486022</td>
     <td class="biobrick_name">BBa_K1486022</td>
-
     <td>rLuc</td>
+
     <td> Renilla Luciferase</td>
     <td>Full renilla luciferase</td>
     <td>Full renilla luciferase</td>
     <td>Control</td>
     <td>Control</td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
<tr>
<tr>
     <td class="biobrick_name">BBa_K1486023</td>
     <td class="biobrick_name">BBa_K1486023</td>
-
     <td>Yeast sfGFP</td>
+
     <td>Yeast optimized superfolder GFP</td>
-
     <td>Superfolder GFP for yeast cells</td>
+
     <td>Yeast optimised superfolder GFP</td>
     <td>Reporter</td>
     <td>Reporter</td>
 +
    <td>Yeast</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486024</td>
     <td class="biobrick_name">BBa_K1486024</td>
-
     <td>Kan</td>
+
     <td>Yeast kanamycin resistance</td>
-
     <td>Yeast kanamycin resistance gene</td>
+
     <td>Yeast kanamycin resistance</td>
     <td>Selection marker</td>
     <td>Selection marker</td>
 +
    <td>Yeast</td>
   </tr>
   </tr>
   <tr>
   <tr>
Line 250: Line 327:
     <td>ADH1 terminator</td>
     <td>ADH1 terminator</td>
     <td>Terminator</td>
     <td>Terminator</td>
-
     <td></td>
+
     <td>Abortion of the transcription at the end of our DNA sequences</td>
 +
    <td>Yeast</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486026</td>
     <td class="biobrick_name">BBa_K1486026</td>
-
     <td>Yeast sfGFP + Kan</td>
+
     <td>sfGFP + Kanamycin resistance for yeast </td>
-
     <td>Yeast sfGFP attached to the yeast kanamycin resistance gene</td>
+
     <td>Tag</td>
-
     <td>Control the expression of pbs2</td>
+
     <td>Control for the expression of Pbs2</td>
 +
    <td>Yeast</td>
   </tr>
   </tr>
<tr>
<tr>
     <td class="biobrick_name">BBa_K1486027</td>
     <td class="biobrick_name">BBa_K1486027</td>
-
     <td>rLuc + Kan</td>
+
     <td>R.reniformis luciferase + ADH1 terminator + Kanamycin resistance</td>
-
     <td>Renilla luciferase attached to the yeast kanamycin resistance gene</td>
+
     <td>Tag</td>
-
     <td></td>
+
    <td>Control for the expression of Pbs2 </td>
 +
     <td>Yeast</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486028</td>
     <td class="biobrick_name">BBa_K1486028</td>
-
     <td>Nterm Yeast sfGFP</td>
+
     <td>Yeast optimized sfGFP N-terminus (1-214)</td>
-
     <td>Nterminal split of the yeast sfGFP</td>
+
     <td>Yeast optimized sfGFP N-terminus (1-214)</td>
-
     <td></td>
+
     <td> </td>
 +
    <td>Yeast</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486029</td>
     <td class="biobrick_name">BBa_K1486029</td>
-
     <td>Nterm sfGFP + ADH1 Terminator + kan</td>
+
     <td>Yeast Optimized sfGFP-N + ADH1 terminator + Kanamycin resistance</td>
-
     <td>Nterm split of yeast sfGFP attached to yeast kanamycin resistance gene</td>
+
     <td>N terminal moiety of split sfGFP</td>
-
     <td></td>
+
     <td>Complementation Assay </td>
 +
    <td>Yeast</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486030</td>
     <td class="biobrick_name">BBa_K1486030</td>
-
     <td>Nterm rLuc + ADH1 Terminator + kan</td>
+
     <td>rLucN + ADH1 terminator + Kanamycin resistance</td>
-
     <td>Nterm split of renilla luciferase attached to yeast kanamycin resistance gene</td>
+
     <td>N terminal moiety of split rLuc</td>
-
     <td></td>
+
    <td>Complementation Assay  </td>
 +
     <td>Yeast</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486031</td>
     <td class="biobrick_name">BBa_K1486031</td>
-
     <td>Ura</td>
+
     <td>CaUra3 selection marker</td>
-
     <td>CDS for Uracil (yeast selective purposes)</td>
+
     <td>selection marker</td>
     <td>Confer resistance to Uracil-deprived medium</td>
     <td>Confer resistance to Uracil-deprived medium</td>
 +
    <td>Yeast</td>
   </tr>
   </tr>
<tr>
<tr>
     <td class="biobrick_name">BBa_K1486032</td>
     <td class="biobrick_name">BBa_K1486032</td>
-
     <td></td>
+
     <td>sfGFP + ADH1 terminator + Ura3 cassette</td>
-
     <td></td>
+
     <td>Tag</td>
-
     <td></td>
+
     <td>Control for the expression of hog1</td>
 +
    <td>Yeast</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486033</td>
     <td class="biobrick_name">BBa_K1486033</td>
-
     <td></td>
+
     <td>sfGFP + ADH1 terminator + Ura3 cassette</td>
-
     <td></td>
+
     <td>Tag</td>
-
     <td></td>
+
     <td>Control for the expression of hog1</td>
 +
    <td>Yeast</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486034</td>
     <td class="biobrick_name">BBa_K1486034</td>
-
     <td></td>
+
     <td>Yeast optimized superfolder GFP C-terminus (215-238)</td>
-
     <td></td>
+
     <td>Yeast optimized superfolder GFP C-terminus (215-238)</td>
-
     <td></td>
+
     <td> </td>
 +
    <td>Yeast</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486035</td>
     <td class="biobrick_name">BBa_K1486035</td>
-
     <td></td>
+
     <td>Yeast Optimized sfGFP-C + ADH1 terminator + CaUra3 Cassette</td>
-
     <td></td>
+
     <td>C terminal moiety of split sfGFP</td>
-
     <td></td>
+
     <td>Complementation Assay </td>
 +
    <td>Yeast</td>
   </tr>
   </tr>
<tr>
<tr>
     <td class="biobrick_name">BBa_K1486036</td>
     <td class="biobrick_name">BBa_K1486036</td>
-
     <td></td>
+
     <td>rLucC + ADH1 terminator + CaUra3 cassette</td>
-
     <td></td>
+
     <td>C terminal moiety of split rLuc</td>
-
     <td></td>
+
     <td>Complementation Assay </td>
 +
    <td>Yeast</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486037</td>
     <td class="biobrick_name">BBa_K1486037</td>
-
     <td></td>
+
     <td>13 amino acids linker [GGGS GGGGS GGGS]</td>
-
     <td></td>
+
     <td>linker</td>
-
     <td></td>
+
     <td>Attach Pbs2 or Hog1 to our different tags and splits </td>
 +
    <td>Yeast</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486038</td>
     <td class="biobrick_name">BBa_K1486038</td>
-
     <td></td>
+
     <td>sfGFPN</td>
-
     <td></td>
+
     <td>N terminus moiety of split superfolder GFP</td>
-
     <td></td>
+
     <td> </td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486039</td>
     <td class="biobrick_name">BBa_K1486039</td>
-
     <td></td>
+
     <td>sfGFPC</td>
-
     <td></td>
+
     <td>C terminus moiety of split superfolder GFP</td>
-
     <td></td>
+
     <td> </td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486040</td>
     <td class="biobrick_name">BBa_K1486040</td>
-
     <td></td>
+
     <td>sfGFPN + CpxR</td>
-
     <td></td>
+
     <td>N terminus moiety of split sfGFP attached to CpxR</td>
-
     <td></td>
+
     <td> </td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486041</td>
     <td class="biobrick_name">BBa_K1486041</td>
-
     <td></td>
+
     <td>sfGFPC + CpxR</td>
-
     <td></td>
+
     <td>C terminus moiety of split sfGFP attached to CpxR</td>
-
     <td></td>
+
     <td> </td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486042</td>
     <td class="biobrick_name">BBa_K1486042</td>
-
     <td></td>
+
     <td>Leucine Zipper</td>
-
     <td></td>
+
     <td>Monomer of leucine zipper TF</td>
-
     <td></td>
+
     <td> </td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486043</td>
     <td class="biobrick_name">BBa_K1486043</td>
-
     <td></td>
+
     <td>Leucine Zipper + split rLuc</td>
-
     <td></td>
+
     <td>Two Leucine Zipper monomers, each attached to a different split rLuc moiety</td>
-
     <td></td>
+
     <td>Control for split rLuc assays</td>
 +
    <td>Bacteria</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="biobrick_name">BBa_K1486044</td>
 +
    <td>IFP[1] Mutated</td>
 +
    <td>Biobrick-compatible IFP[1]</td>
 +
    <td> </td>
 +
    <td>Bacteria</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="biobrick_name">BBa_K1486045</td>
 +
    <td>IFP[2] Mutated</td>
 +
    <td>Biobrick-compatible IFP[2]</td>
 +
    <td> </td>
 +
    <td>Bacteria</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="biobrick_name">BBa_K1486046</td>
 +
    <td>CpxR promoter FW</td>
 +
    <td>CpxR binding-region in forward direction</td>
 +
    <td> </td>
 +
    <td>Bacteria</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="biobrick_name">BBa_K1486047</td>
 +
    <td>CpxR promoter RV</td>
 +
    <td>CpxR binding-region in reverse direction</td>
 +
    <td> </td>
 +
    <td>Bacteria</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="biobrick_name">BBa_K1486048</td>
 +
    <td>CpxR reporter</td>
 +
    <td>Calgary's CpxR reporter repaired (sequence was missing)</td>
 +
    <td>To see when CpxR is active</td>
 +
    <td>Bacteria</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="biobrick_name">BBa_K1486049</td>
 +
    <td>CpxR promoter FW + RFP</td>
 +
    <td>Reporter of CpxR</td>
 +
    <td>Test the direction of the complete CpxR promoter</td>
 +
    <td>Bacteria</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="biobrick_name">BBa_K1486050</td>
 +
    <td>CpxR promoter RV + RFP</td>
 +
    <td>Reporter of CpxR</td>
 +
    <td>Test the direction of the complete CpxR promoter</td>
 +
    <td>Bacteria</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="biobrick_name">BBa_K1486052</td>
 +
    <td>Spacer</td>
 +
    <td>40 bases placed between constructs</td>
 +
    <td>Separate two constructs in the same plasmid</td>
 +
    <td>Bacteria</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="biobrick_name">BBa_K1486053</td>
 +
    <td>10 aa linker</td>
 +
    <td>10 amino-acid linker</td>
 +
    <td>Attach CheY/Z to split luciferases</td>
 +
    <td>Bacteria</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="biobrick_name">BBa_K1486054</td>
 +
    <td>CheY/CheZ + split rLuc</td>
 +
    <td>CheY and CheZ, each attached to a moiety of split renilla luciferase</td>
 +
    <td>Positive control for the split rLuc</td>
 +
    <td>Bacteria</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="biobrick_name">BBa_K1486055</td>
 +
    <td>CheY/CheZ + split fLuc</td>
 +
    <td>CheY and CheZ, each attached to a moiety of split firefly luciferase</td>
 +
    <td>Positive control for the split fLuc</td>
 +
    <td>Bacteria</td>
 +
  </tr>
 +
  <tr>
 +
    <td class="biobrick_name">BBa_K1486056</td>
 +
    <td>CpxR & Split IFP1.4 [Cterm + Cterm][2]</td>
 +
    <td>Two CpxR CDS, each C terminus attached to a moiety of the biobrick-compatible IFP</td>
 +
    <td>Characterize CpxR dimerization</td>
 +
    <td>Bacteria</td>
   </tr>
   </tr>
</table>
</table>
 +
</section>
<br /><br />
<br /><br />
 +
 +
<section id="microfluidics">
 +
<h3 class="section-heading">Microfluidics parts (chips created)</h3>
 +
<p class="lead">
 +
Our team designed and made 4 microfluidic chips. At the beginning, we also used the <a target="_blank" href="http://link.springer.com/protocol/10.1007%2F978-1-61779-292-2_6">MITOMI chip</a>.</p>
 +
<p class="lead">When designing the chips, the team took into account the future users and the current iGEM classification of parts. We considered it best to construct our chips as composite microfluidic parts, so their sub-parts could be used and combined in multiple ways. The flow and control layers can be separated and reused, as well as all the basic structures (chamber + connecting channel), nodes, array parts,...</p>
 +
 +
<p>By clicking on each of the designs' name, you can download the original files.</p>
 +
<!-- send all lines here: https://2014.igem.org/Team:EPF_Lausanne/Microfluidics/Designing -->
 +
<table class="table table-striped table-hover" id="chips_list">
 +
  <tr>
 +
    <th>Name</th>
 +
    <th>Main Function</th>
 +
  </tr>
 +
  <tr>
 +
    <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/b/b7/SmashColi_iGEM_EPFL_2014.zip">SmashColi</a></td>
 +
    <td>To be able to separate the chip in 4 different compartments and apply 4 different pressures on each row of chambers.</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/3/33/TheBioPad_iGEM_EPFL_2014.zip">The BioPad</a></td>
 +
    <td>A large and simple microfluidic chip containing 6400 chambers in which the cells are contained in. Each chamber acts as a pixel for the BioPad project.</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/b/b3/CleanColi_iGEM_EPFL_2014.zip">CleanColi</a></td>
 +
    <td>As a result of our Safety page, we decided to create a chip that is able to seal the bacteria in the chip, preventing them to leave the chip.</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/7/79/FilterColi_iGEM_EPFL_2014.zip">FilterColi</a></td>
 +
    <td>To successfully immerse cells in a certain solution, this chip was designed to flow in the new medium in the chambers instead of doing it by diffusion.</td>
 +
  </tr>
 +
</table>
 +
</section>
 +
 +
 +
<br /><br />
</div>
</div>
</div>
</div>
 +
</div>
</div>
</div>
 +
<div class="col col-md-3">
 +
<nav id="affix-nav" class="sidebar hidden-sm hidden-xs">
 +
    <ul class="nav sidenav box" data-spy="affix" data-offset-top="200" data-offset-bottom="400">
 +
        <li class="active"><a href="#dna">DNA Parts</a></li>
 +
        <li><a href="#microfluidics">Microfluidics Parts</a></li>
 +
    </ul>
 +
</nav>
 +
</div>
 +
</div>
 +
</div>
<!-- END ABSTRACT -->
<!-- END ABSTRACT -->
 +
<script type="text/javascript">
 +
    $(document).ready(function() {
 +
      $('#biobricks_list tr').click(function (e) {
 +
        text = $(this).children('td.biobrick_name').first().text();
 +
        if (text != '') {
 +
          return window.open('http://parts.igem.org/Part:' + text, '_blank');
 +
        }
 +
      });
 +
    });
 +
</script>
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</html>
{{CSS/EPFL_bottom}}
{{CSS/EPFL_bottom}}

Latest revision as of 01:45, 18 October 2014

PARTS


DNA parts submitted by the 2014 EPFL iGEM team

Our team submitted a total of 55 Biobricks (part 51 does not exist).

In addition, 4 microfluidic designs have also been submitted to the registry.

Biobrick What it is Function Why do we use it? Group
BBa_K1486000 CpxR coding sequence Transcription factor To make most of our biobricks! Bacteria
BBa_K1486001 Arabinose promoter + CpxR Transcription factor Bacteria
BBa_K1486002 Arabinose promoter + sfGFP-CpxR [Nterm] Expresses fused protein Test CpxR expression & Ara promoter Bacteria
BBa_K1486003 Flexible linker 2x (GGGS) Attaches two proteins together Bacteria
BBa_K1486004 Flexible linker 2x (GGGGS) Attaches two proteins together Bacteria
BBa_K1486005 Arabinose promoter + sfGFP-CpxR [Cterm] Expresses fused protein Test CpxR expression & Ara promoter Bacteria
BBa_K1486006 IFP[1] N terminus of split IFP Bacteria
BBa_K1486007 IFP[2] C terminus of split IFP Bacteria
BBa_K1486008 CpxR & Split IFP1.4 [Cterm + Cterm] Two CpxR CDS, each C terminus attached to a moiety of IFP Characterize CpxR dimerization Bacteria
BBa_K1486009 CpxR & Split IFP1.4 [Nterm + Nterm] Two CpxR CDS, each N terminus attached to a moiety of IFP Characterize CpxR dimerization Bacteria
BBa_K1486010 CpxR & Split IFP1.4 [Nterm + Cterm] Two CpxR CDS, each attached to a moiety of IFP Characterize CpxR dimerization Bacteria
BBa_K1486011 CpxR & Split IFP1.4 [Cterm + Nterm] Two CpxR CDS, each attached to a moiety of IFP Characterize CpxR dimerization Bacteria
BBa_K1486012 CpxR-IFP[1] under pBAD CpxR with the Nterm moiety of IFP attached at its C terminus Intermediate & control Bacteria
BBa_K1486013 CpxR-IFP[2] under pBAD CpxR with the Cterm moiety of IFP attached at its C terminus Intermediate & control Bacteria
BBa_K1486014 IFP[1]-CpxR under pBAD CpxR with the Nterm moiety of IFP attached at its N terminus Intermediate & control Bacteria
BBa_K1486015 IFP[2]-CpxR under pBAD CpxR with the Cterm moiety of IFP attached at its N terminus Intermediate & control Bacteria
BBa_K1486016 fLuc[1] N terminus moiety of the firefly luciferase Bacteria
BBa_K1486017 fLuc[2] C terminus moiety of the firefly luciferase Bacteria
BBa_K1486018 Arabinose promoter + fLuc[1] + fLuc[2] Split firefly luciferase under arabinose promoter Control Bacteria
BBa_K1486019 rLuc[1] C terminus moiety of the renilla luciferase Bacteria
BBa_K1486020 rLuc[2] N terminus moiety of the renilla luciferase Bacteria
BBa_K1486021 Arabinose promoter + rLuc[1] + rLuc[2] Split renilla luciferase under arabinose promoter Control Bacteria
BBa_K1486022 Renilla Luciferase Full renilla luciferase Control Bacteria
BBa_K1486023 Yeast optimized superfolder GFP Yeast optimised superfolder GFP Reporter Yeast
BBa_K1486024 Yeast kanamycin resistance Yeast kanamycin resistance Selection marker Yeast
BBa_K1486025 ADH1 terminator Terminator Abortion of the transcription at the end of our DNA sequences Yeast
BBa_K1486026 sfGFP + Kanamycin resistance for yeast Tag Control for the expression of Pbs2 Yeast
BBa_K1486027 R.reniformis luciferase + ADH1 terminator + Kanamycin resistance Tag Control for the expression of Pbs2 Yeast
BBa_K1486028 Yeast optimized sfGFP N-terminus (1-214) Yeast optimized sfGFP N-terminus (1-214) Yeast
BBa_K1486029 Yeast Optimized sfGFP-N + ADH1 terminator + Kanamycin resistance N terminal moiety of split sfGFP Complementation Assay Yeast
BBa_K1486030 rLucN + ADH1 terminator + Kanamycin resistance N terminal moiety of split rLuc Complementation Assay Yeast
BBa_K1486031 CaUra3 selection marker selection marker Confer resistance to Uracil-deprived medium Yeast
BBa_K1486032 sfGFP + ADH1 terminator + Ura3 cassette Tag Control for the expression of hog1 Yeast
BBa_K1486033 sfGFP + ADH1 terminator + Ura3 cassette Tag Control for the expression of hog1 Yeast
BBa_K1486034 Yeast optimized superfolder GFP C-terminus (215-238) Yeast optimized superfolder GFP C-terminus (215-238) Yeast
BBa_K1486035 Yeast Optimized sfGFP-C + ADH1 terminator + CaUra3 Cassette C terminal moiety of split sfGFP Complementation Assay Yeast
BBa_K1486036 rLucC + ADH1 terminator + CaUra3 cassette C terminal moiety of split rLuc Complementation Assay Yeast
BBa_K1486037 13 amino acids linker [GGGS GGGGS GGGS] linker Attach Pbs2 or Hog1 to our different tags and splits Yeast
BBa_K1486038 sfGFPN N terminus moiety of split superfolder GFP Bacteria
BBa_K1486039 sfGFPC C terminus moiety of split superfolder GFP Bacteria
BBa_K1486040 sfGFPN + CpxR N terminus moiety of split sfGFP attached to CpxR Bacteria
BBa_K1486041 sfGFPC + CpxR C terminus moiety of split sfGFP attached to CpxR Bacteria
BBa_K1486042 Leucine Zipper Monomer of leucine zipper TF Bacteria
BBa_K1486043 Leucine Zipper + split rLuc Two Leucine Zipper monomers, each attached to a different split rLuc moiety Control for split rLuc assays Bacteria
BBa_K1486044 IFP[1] Mutated Biobrick-compatible IFP[1] Bacteria
BBa_K1486045 IFP[2] Mutated Biobrick-compatible IFP[2] Bacteria
BBa_K1486046 CpxR promoter FW CpxR binding-region in forward direction Bacteria
BBa_K1486047 CpxR promoter RV CpxR binding-region in reverse direction Bacteria
BBa_K1486048 CpxR reporter Calgary's CpxR reporter repaired (sequence was missing) To see when CpxR is active Bacteria
BBa_K1486049 CpxR promoter FW + RFP Reporter of CpxR Test the direction of the complete CpxR promoter Bacteria
BBa_K1486050 CpxR promoter RV + RFP Reporter of CpxR Test the direction of the complete CpxR promoter Bacteria
BBa_K1486052 Spacer 40 bases placed between constructs Separate two constructs in the same plasmid Bacteria
BBa_K1486053 10 aa linker 10 amino-acid linker Attach CheY/Z to split luciferases Bacteria
BBa_K1486054 CheY/CheZ + split rLuc CheY and CheZ, each attached to a moiety of split renilla luciferase Positive control for the split rLuc Bacteria
BBa_K1486055 CheY/CheZ + split fLuc CheY and CheZ, each attached to a moiety of split firefly luciferase Positive control for the split fLuc Bacteria
BBa_K1486056 CpxR & Split IFP1.4 [Cterm + Cterm][2] Two CpxR CDS, each C terminus attached to a moiety of the biobrick-compatible IFP Characterize CpxR dimerization Bacteria


Microfluidics parts (chips created)

Our team designed and made 4 microfluidic chips. At the beginning, we also used the MITOMI chip.

When designing the chips, the team took into account the future users and the current iGEM classification of parts. We considered it best to construct our chips as composite microfluidic parts, so their sub-parts could be used and combined in multiple ways. The flow and control layers can be separated and reused, as well as all the basic structures (chamber + connecting channel), nodes, array parts,...

By clicking on each of the designs' name, you can download the original files.

Name Main Function
SmashColi To be able to separate the chip in 4 different compartments and apply 4 different pressures on each row of chambers.
The BioPad A large and simple microfluidic chip containing 6400 chambers in which the cells are contained in. Each chamber acts as a pixel for the BioPad project.
CleanColi As a result of our Safety page, we decided to create a chip that is able to seal the bacteria in the chip, preventing them to leave the chip.
FilterColi To successfully immerse cells in a certain solution, this chip was designed to flow in the new medium in the chambers instead of doing it by diffusion.


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