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Team:Bordeaux/NotebookAugust18
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<div class="type-exp"> Molecular biology</div> | <div class="type-exp"> Molecular biology</div> | ||
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Buffer 5X 10μl<br> | Buffer 5X 10μl<br> | ||
H2O qsp 50μl<br> | H2O qsp 50μl<br> | ||
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PCR program<br> | PCR program<br> | ||
3min 95°C<br> | 3min 95°C<br> | ||
25 cycles : <br> | 25 cycles : <br> | ||
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30sec 95°C<br> | 30sec 95°C<br> | ||
30sec 45°C <br> | 30sec 45°C <br> | ||
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</li> | </li> | ||
</ul> | </ul> | ||
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Preculture of ELP-60 in 500ml TB medium</div> | Preculture of ELP-60 in 500ml TB medium</div> | ||
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Latest revision as of 01:44, 18 October 2014
18th August
Molecular biology
-
Chewback: linearization of ELP-40
ELP-40 miniprep product 1μl
Primer ELP-40 forward 2,5μl
Primer ELP-40 reverse 2,5μl
dNTP (10mM) 1μl
enzyme 0,5μl
Buffer 5X 10μl
H2O qsp 50μl
PCR program
3min 95°C
25 cycles :
30sec 95°C5min 72°C
30sec 45°C
3min 72°C
Migration on agarose 1% gel (30min, 100V)
Production
Preculture of ELP-60 in 500ml TB medium
