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Team:HokkaidoU Japan/Notebook/H Stem/Replacement To 1A3
From 2014.igem.org
(Difference between revisions)
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<h2 class="step-title"> | <h2 class="step-title"> | ||
| - | + | Mini-Prep | |
</h2> | </h2> | ||
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</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
| - | + | R0010-stem-as-stem-B0015 (on pSB1A3) | |
| + | |||
</div> | </div> | ||
</div> | </div> | ||
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</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
| - | + | R0010-stem-as-stem-B0015(on pSB1C3) with 100up_F Tm:62.6℃ & 200dn_R Tm:66.6℃ | |
</div> | </div> | ||
</div> | </div> | ||
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</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
| - | + | pSB1A3 with EcoRI & PstI, | |
| + | R0010-stem-as-stem-B0015 with EcoRI & PstI | ||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
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</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
| - | + | 0010-stem-as-stem-B0015 & pSB1A3 | |
</div> | </div> | ||
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<div class="step-recipe"> | <div class="step-recipe"> | ||
| - | + | R0010-stem-as-stem-B0015(on pSB1A3) | |
| + | 5.0µL to DH5alpha; | ||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 01:37, 18 October 2014
Notebook
Lab Documents
H-Stem Vector Exchange to pSB1A3
-
Start
-
Mini-Prep
R0010-stem-as-stem-B0015 (on pSB1A3) -
PCR
R0010-stem-as-stem-B0015(on pSB1C3) with 100up_F Tm:62.6℃ & 200dn_R Tm:66.6℃ -
Digestion
pSB1A3 with EcoRI & PstI, R0010-stem-as-stem-B0015 with EcoRI & PstI -
Ligation
0010-stem-as-stem-B0015 & pSB1A3 -
Transformation
R0010-stem-as-stem-B0015(on pSB1A3) 5.0µL to DH5alpha; -
Complete!
