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Team:TU Darmstadt/Notebook/Methods/Methanol extraction
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| - | <!--TYPO3SEARCH_begin--><div id="c248" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">Methanol | + | <!--TYPO3SEARCH_begin--><div id="c248" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">Methanol Extraction</h1></div><div class="csc-textpic-text"><div><p><b>Materials: </b></p></div><div><p>- Methanol</p></div><div><p>- 37% hydrochloric acid</p></div><div><p>- Centrifuge</p></div><div><p>- Tubes</p></div><div></div><div><p><b>Procedure:</b></p></div><div><p>- Spin down the bacterial solution at 4500g for 10 minutes.</p></div><div><p>- Remove culture media, weigh the remaining pellet, add the same amount of methanol and vortex for 5 minutes. Separate supernatant and pellet and acetify each with about 300µl of 37% hydrochloric acid till the color turns to red. </p></div><div><p>- Spin down the resolved pellet at 13,3g for 10 minutes and Remove supernatant.</p></div><div><p>The anthocyanidin is present in the organic and the aqueous phase; the color can be changed by adjusting the pH-value. </p></div><div></div></div></div><!--TYPO3SEARCH_end--> |
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Latest revision as of 00:50, 18 October 2014
Methanol Extraction
Materials:
- Methanol
- 37% hydrochloric acid
- Centrifuge
- Tubes
Procedure:
- Spin down the bacterial solution at 4500g for 10 minutes.
- Remove culture media, weigh the remaining pellet, add the same amount of methanol and vortex for 5 minutes. Separate supernatant and pellet and acetify each with about 300µl of 37% hydrochloric acid till the color turns to red.
- Spin down the resolved pellet at 13,3g for 10 minutes and Remove supernatant.
The anthocyanidin is present in the organic and the aqueous phase; the color can be changed by adjusting the pH-value.
