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Team:TU Darmstadt/Notebook/Methods/DNA quantification with NanoDrop
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| - | <!--TYPO3SEARCH_begin--><div id="c87" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">DNA | + | <!--TYPO3SEARCH_begin--><div id="c87" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">DNA Quantification With NanoDrop</h1></div><div><p><b>Equipment:</b></p></div><div><p>- Micropipettes with sterile tips</p></div><div><p>- Nanodrop</p></div><div></div><div><p><b>Chemicals & consumables:</b> </p></div><div><p>- DNA probe</p></div><div><p>- nuclease free H<sub>2</sub>O</p></div><div></div><div><p><b>Procedure:</b></p></div><div><p>1. Start the programme for the NanoDrop and click on "Nucleic Acids"</p></div><div><p>2. Pipette 1 µL of a water sample onto the measuring surface and click “OK” for initialising</p></div><div><p>3. Load your blank and click "Blank"</p></div><div><p>4. Load your DNA samples and click "Measure"</p></div><div></div><div><p>The system calculate automatically the 260/280 value and the 260/230 value.</p></div></div><!--TYPO3SEARCH_end--> |
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Latest revision as of 00:33, 18 October 2014
DNA Quantification With NanoDrop
Equipment:
- Micropipettes with sterile tips
- Nanodrop
Chemicals & consumables:
- DNA probe
- nuclease free H2O
Procedure:
1. Start the programme for the NanoDrop and click on "Nucleic Acids"
2. Pipette 1 µL of a water sample onto the measuring surface and click “OK” for initialising
3. Load your blank and click "Blank"
4. Load your DNA samples and click "Measure"
The system calculate automatically the 260/280 value and the 260/230 value.
