Team:TU Darmstadt/Notebook/Methods/Agarose gel electrophoresis

From 2014.igem.org

(Difference between revisions)
(Created page with "{{:Team:TU_Darmstadt/Template}} <html> <div id="contentWrap" class="container_24"> <div id="breadcrumbs" class="grid_24"> <p>Sie sind hier:  <a href="index.php...")
 
(14 intermediate revisions not shown)
Line 5: Line 5:
<div id="contentWrap" class="container_24">
<div id="contentWrap" class="container_24">
-
<div id="breadcrumbs" class="grid_24">
+
-
<p>Sie sind hier:&nbsp; <a href="index.php?id=23" >wiki</a> &rsaquo;&nbsp;<a href="index.php?id=32" >Notebook</a> &rsaquo;&nbsp;<a href="index.php?id=54" >Methods</a> &rsaquo;&nbsp;<span class="current"><a href="index.php?id=84" >Agarose gel electrophoresis</a></span></p>
+
-
</div>
+
-
+
<div id="leftNavi" class="grid_5">
<div id="leftNavi" class="grid_5">
<nav>
<nav>
-
<ul class="menu"><li class="first"><a href="index.php?id=160" >Home</a></li><li><a href="index.php?id=28" >Project</a></li><li><a href="index.php?id=29" >Results</a></li><li><a href="index.php?id=30" >Policy & Practices</a></li><li><a href="index.php?id=31" >Achievements</a></li><li class="active"><a href="index.php?id=32" >Notebook</a><ul class="menu2"><li class="first"><a href="index.php?id=53" >Labjournal</a></li><li><a href="index.php?id=73" >Materials</a></li><li class="active last"><a href="index.php?id=54" >Methods</a><ul class="menu3"><li class="active first"><a href="index.php?id=84" >Agarose gel electrophoresis</a></li><li><a href="index.php?id=85" >Dephosphorylation</a></li><li><a href="index.php?id=86" >DNA ligation</a></li><li><a href="index.php?id=87" >DNA quantification with NanoDrop</a></li><li><a href="index.php?id=89" >PCR purification</a></li><li><a href="index.php?id=90" >PCR with Pfu polymerase</a></li><li><a href="index.php?id=91" >Plasmid preparation</a></li><li><a href="index.php?id=92" >Restriction digest</a></li><li><a href="index.php?id=93" >SDS-PAGE</a></li><li><a href="index.php?id=94" >Bacterial cell culture</a></li><li><a href="index.php?id=95" >Cell counting/plating</a></li><li><a href="index.php?id=96" >Chemically competent cells</a></li><li><a href="index.php?id=97" >Colony PCR with Taq polymerase</a></li><li><a href="index.php?id=98" >Glycerine stocks</a></li><li><a href="index.php?id=99" >Heat shock transformation</a></li><li><a href="index.php?id=100" >Protein expression</a></li><li><a href="index.php?id=132" >37% hydrochloric acid extraction</a></li><li><a href="index.php?id=131" >Dichloromethane extraction</a></li><li><a href="index.php?id=134" >Methanol extraction</a></li><li class="last"><a href="index.php?id=135" >Ethanol extraction</a></li></ul></li></ul></li><li><a href="index.php?id=33" >Team</a></li><li class="last"><a href="index.php?id=34" >Gallery</a></li></ul>
+
<ul class="menu"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt" >Home</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Project" >Project</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results" >Results</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/PolicyandPractices" >Policy & Practices</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Achievements" >Achievements</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook" >Notebook</a><ul class="menu2"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal" >Labjournal</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials" >Materials</a></li><li class="active last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods" >Methods</a><ul class="menu3"><li class="active first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Agarose_gel_electrophoresis" >Agarose gel electrophoresis</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Dephosphorylation" >Dephosphorylation</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/DNA_ligation" >DNA ligation</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/DNA_quantification_with_NanoDrop" >DNA quantification with NanoDrop</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/PCR_purification" >PCR purification</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/PCR_with_Pfu_polymerase" >PCR with Pfu polymerase</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Plasmid_preparation" >Plasmid preparation</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Restriction_digest" >Restriction digest</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/SDS-PAGE" >SDS-PAGE</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Bacterial_cell_culture" >Bacterial cell culture</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Cell_counting_plating" >Cell counting/plating</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Chemically_competent_cells" >Chemically competent cells</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Colony_PCR_with_Taq_polymerase" >Colony PCR with Taq polymerase</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Glycerine_stocks" >Glycerine stocks</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Heat_shock_transformation" >Heat shock transformation</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Protein expression" >Protein expression</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/hydrochloric_acid_extraction" >37% hydrochloric acid extraction</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Dichloromethane_extraction" >Dichloromethane extraction</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Methanol_extraction" >Methanol extraction</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Ethanol_extraction" >Ethanol extraction</a></li></ul></li></ul></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Team" >Team</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Gallery" >Gallery</a></li></ul>
</nav>
</nav>
</div>
</div>
-
<div id="content" class="grid_19">
+
<div id="wikicontent" class="grid_19">
-
<!--TYPO3SEARCH_begin--><div id="c83" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">Agarose gel electrophoresis</h1></div><div><p><b>Short explanation:</b></p></div><div><p>Agarose gel electrophoresis is the most common used method to separate nucleic acids. Due to their negativ charge DNA and RNA molecules can be moved through an agarose gel by an electric field (electrophoresis). Longer molecules move slower through the agarose matrix while short move faster and migrate further.</p></div><div></div><div><p><b>Procedure:</b></p></div><div><p>In common we used 1% agarose gel.</p></div><div><p>1. The hot and liquid agarose gel is mixed with 4 µl NANCY for 50 ml of agarosegel (Shake in a bottle)</p></div><div><p>2. Fill everything in a chamber and let it cool down (do not forget the well combs)</p></div><div><p>3. Take 1xTAE-Buffer for the run</p></div><div><p>4. In the first ten minutes run the gel with 80 V and afterwards select 120V</p></div></div><!--TYPO3SEARCH_end-->
+
<!--TYPO3SEARCH_begin--><div id="c83" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">Agarose Gel Electrophoresis</h1></div><div><p><b>Short Explanation:</b></p></div><div><p>Agarose gel electrophoresis is the most common used method to separate nucleic acids. Due to their negativ charge DNA and RNA molecules can be moved through an agarose gel by an electric field (electrophoresis). Longer molecules move slower through the agarose matrix while short move faster and migrate further.</p></div><div></div><div><p><b>Procedure:</b></p></div><div><p>In common we used 1% agarose gel.</p></div><div><p>1. The hot and liquid agarose gel is mixed with 4 µl NANCY for 50 ml of agarosegel (Shake in a bottle)</p></div><div><p>2. Fill everything in a chamber and let it cool down (do not forget the well combs)</p></div><div><p>3. Take 1xTAE-Buffer for the run</p></div><div><p>4. In the first ten minutes run the gel with 80 V and afterwards select 120V</p></div></div><!--TYPO3SEARCH_end-->
</div>
</div>

Latest revision as of 00:30, 18 October 2014

Home


Agarose Gel Electrophoresis

Short Explanation:

Agarose gel electrophoresis is the most common used method to separate nucleic acids. Due to their negativ charge DNA and RNA molecules can be moved through an agarose gel by an electric field (electrophoresis). Longer molecules move slower through the agarose matrix while short move faster and migrate further.

Procedure:

In common we used 1% agarose gel.

1. The hot and liquid agarose gel is mixed with 4 µl NANCY for 50 ml of agarosegel (Shake in a bottle)

2. Fill everything in a chamber and let it cool down (do not forget the well combs)

3. Take 1xTAE-Buffer for the run

4. In the first ten minutes run the gel with 80 V and afterwards select 120V