Team:TU Darmstadt/Notebook/Methods
From 2014.igem.org
(Difference between revisions)
(One intermediate revision not shown) | |||
Line 5: | Line 5: | ||
<div id="contentWrap" class="container_24"> | <div id="contentWrap" class="container_24"> | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
<div id="leftNavi" class="grid_5"> | <div id="leftNavi" class="grid_5"> | ||
<nav> | <nav> | ||
Line 45: | Line 42: | ||
<p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/SDS-PAGE" title="Opens internal link in current window" class="internal-link">SDS-PAGE</a> | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/SDS-PAGE" title="Opens internal link in current window" class="internal-link">SDS-PAGE</a> | ||
</p> | </p> | ||
- | <p> | + | <p></p> |
<p><b>In vivo methods</b> | <p><b>In vivo methods</b> | ||
</p> | </p> | ||
Line 60: | Line 57: | ||
<p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Heat_shock_transformation" title="Opens internal link in current window" class="internal-link">Heat shock transformation</a> | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Heat_shock_transformation" title="Opens internal link in current window" class="internal-link">Heat shock transformation</a> | ||
</p> | </p> | ||
- | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Protein_expression" title="Opens internal link in current window" class="internal-link">Protein expression</a></p></header><ul style="line-height: 1.5em; list-style: none; margin: 0.3em 50px 0px; padding: 0px; text-align: justify; "></ul><p style="margin: 0px 50px 15px; line-height: 1.5em; text-align: justify; color: white; font-family: 'Arial regular'; font-size: medium; " | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Protein_expression" title="Opens internal link in current window" class="internal-link">Protein expression</a></p></header><ul style="line-height: 1.5em; list-style: none; margin: 0.3em 50px 0px; padding: 0px; text-align: justify; "></ul><p style="margin: 0px 50px 15px; line-height: 1.5em; text-align: justify; color: white; font-family: 'Arial regular'; font-size: medium; "</p></div><div id="c245" class="csc-default"><p><b>Anthocyanin extraction methods</b> |
</p> | </p> | ||
<p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/37_hydrochloric_acid" title="Opens internal link in current window" class="internal-link">37% hydrochloric acid</a> | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/37_hydrochloric_acid" title="Opens internal link in current window" class="internal-link">37% hydrochloric acid</a> |
Latest revision as of 00:28, 18 October 2014
Methods
This page outlines our standardized protocols we used in the iGEM project 2014. If we changed one of the protocols for a specific reason, we mentioned it in the laboratory notebook.
In vitro methods
For DNA
- DNA quantification with NanoDrop
For Proteins
- SDS-PAGE
In vivo methods