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Team:UC Davis/Signal Processing
From 2014.igem.org
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To model our system, we first focused our attention on the linear range of each enzymes Michaelis Menten plot. The linear range of this plot is governed by the relationship:<br></p> | To model our system, we first focused our attention on the linear range of each enzymes Michaelis Menten plot. The linear range of this plot is governed by the relationship:<br></p> | ||
<div style="margin:auto;display:block;float:center"> | <div style="margin:auto;display:block;float:center"> | ||
| - | <img src="https://static.igem.org/mediawiki/2014/4/41/SingStarterRight.png" style="margin-left:auto;margin-right:auto;border:1.5px solid #212f20;"/></div><br> | + | <img src="https://static.igem.org/mediawiki/2014/4/41/SingStarterRight.png" style="margin-left:auto;margin-right:auto;border:1.5px solid #212f20;"/> |
| - | <p>This was useful, but Olive Oil contains many aldehyes and the enzymatic response is different for each one.<br> | + | </div><br> |
| - | <img src="https://static.igem.org/mediawiki/2014/b/b5/RelativeVelocity.png" style="margin-left:auto;margin-right:auto;"/><br> | + | <p>This was useful, but Olive Oil contains many aldehyes and the enzymatic response is different for each one.</p><br> |
| - | To describe this mathematically we started with a simple assumption that none of the substrates would induce competitive inhibition. If this was the case, the modeling would be simple. We would consider the observed velocity to be a linear combination of the three singular responses to aldehyde.</p><br> | + | <div style="margin:auto;display:block;float:center"> |
| + | <img src="https://static.igem.org/mediawiki/2014/b/b5/RelativeVelocity.png" style="margin-left:auto;margin-right:auto;"/></div><br> | ||
| + | |||
| + | <p>To describe this mathematically we started with a simple assumption that none of the substrates would induce competitive inhibition. If this was the case, the modeling would be simple. We would consider the observed velocity to be a linear combination of the three singular responses to aldehyde.</p><br> | ||
<div style="margin-left:auto;margin-right:auto;"> | <div style="margin-left:auto;margin-right:auto;"> | ||
<img src="https://static.igem.org/mediawiki/2014/5/56/ThreeSubstrates.png" style="border:1.5px solid #212f20;"/></div> | <img src="https://static.igem.org/mediawiki/2014/5/56/ThreeSubstrates.png" style="border:1.5px solid #212f20;"/></div> | ||
Revision as of 00:24, 18 October 2014
Signal Processing
To model our system, we first focused our attention on the linear range of each enzymes Michaelis Menten plot. The linear range of this plot is governed by the relationship:
This was useful, but Olive Oil contains many aldehyes and the enzymatic response is different for each one.
To describe this mathematically we started with a simple assumption that none of the substrates would induce competitive inhibition. If this was the case, the modeling would be simple. We would consider the observed velocity to be a linear combination of the three singular responses to aldehyde.
To test our model we built a set of 64 difference combinations
