Team:UESTC-China/Protocol

From 2014.igem.org

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             <td class="lastmid">Qualitative detection</td>
             <td class="lastmid">Qualitative detection</td>
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             <td class="lastmid">Have 6 positive seedlings from every transgenic line (about 8 leaves age) , and 6 wild-type seedings with the same growth equally distributed into three 650ml culture bottles. Treat with 10µl 37% formaldehyde for a week. </td>
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             <td class="lastmid">Have 6 positive seedlings from every transgenic line (about 8 leaves age) , and 6 wild-type seedings with the same growth equally distributed into three 650ml culture bottles. Treat with 10µl 37% formaldehyde for two weeks. </td>
<td class="mid"></td>
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             <td class="lastmid"><br>Quantitative detection</td>
             <td class="lastmid"><br>Quantitative detection</td>
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             <td class="lastmid"><br> Have 4 positive seedlings from every transgenic line (about 8 leaves age) in a 650ml culture bottle. Treat with 10µl 37% formaldehyde for 2 weeks. Using a formaldehyde detector (Gastec Passive Dositube, 91D, Ayase, Kanagawa, Japan) was set in the hole to detect gaseous formaldehyde. Three and a half hours later, the measurement was stopped and the results were photographed <i>(Chen et al., 2010)</i>.</td>
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             <td class="lastmid"><br> Have 4 positive seedlings from every transgenic line (about 8 leaves age) in a 650ml culture bottle. Treat with 10µl 37% formaldehyde for 3 weeks. Using a formaldehyde detector (Gastec Passive Dositube, 91D, Ayase, Kanagawa, Japan) was set in the hole to detect gaseous formaldehyde. Three and a half hours later, the measurement was stopped and the results were photographed <i>(Chen et al., 2010)</i>.</td>
            <td class="lastmid"><br>Put 4 wild -type seedlings with the same growth of seedlings in experimental group into 650ml culture bottle , with same processing as the case of the experimental group .</td>
            <td class="lastmid"><br>Put 4 wild -type seedlings with the same growth of seedlings in experimental group into 650ml culture bottle , with same processing as the case of the experimental group .</td>

Revision as of 00:04, 18 October 2014

UESTC-China