Team:Tsinghua/Introduction

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       <li><a href="#">MAIN PAGE</a></li>
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       <li><a href="https://2014.igem.org/Team:Tsinghua/Main_page">MAIN PAGE</a></li>
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       <li><a href="#">INTRODUCTION</a>
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       <li><a href="https://2014.igem.org/Team:Tsinghua/Introduction">INTRODUCTION</a>
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           <li><a href="#">Navigation</a></li>     
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           <li><a href="#">Diabetes Type I</a></li>      
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           <li><a href="https://2014.igem.org/Team:Tsinghua/Introduction">Overview</a></li>     
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           <li><a href="#">Gene Therapy</a></li>
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           <li><a href="https://2014.igem.org/Team:Tsinghua/Introduction/Diabetes">Diabetes Type I</a></li>
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           <li><a href="https://2014.igem.org/Team:Tsinghua/Introduction/Gene_therapy">Gene Therapy</a></li>
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       <li><a href="#">PROJECT</a>
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       <li><a href="https://2014.igem.org/Team:Tsinghua/Project">PROJECT</a>
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          <li><a href="#">Navigation</a></li>     
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          <li><a href="#">The Virus</a></li>     
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<li><a href="https://2014.igem.org/Team:Tsinghua/Project">Overview</a></li>     
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          <li><a href="#">The Drug</a></li>
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<li><a href="https://2014.igem.org/Team:Tsinghua/Project/Virus">The Virus</a></li>     
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<li><a href="https://2014.igem.org/Team:Tsinghua/Project/Drug">The Drug</a></li>
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          <li><a href="#">The Cocktail</a></li>
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<li><a href="https://2014.igem.org/Team:Tsinghua/Project/Microbe">The Microbe</a></li>
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<li><a href="https://2014.igem.org/Team:Tsinghua/Project/Cocktail">The Cocktail</a></li>
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       <li><a href="https://2014.igem.org/Team:Tsinghua/Modeling">Modeling</a></li>
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       <li><a href="#">NOTEBOOK</a>
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       <li><a href="https://2014.igem.org/Team:Tsinghua/Parts">Parts</a></li>
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          <li><a href="#">PROTOCOL</a></li>
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          <li><a href="#">LABLOG</a></li>
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       <li><a href="https://2014.igem.org/Team:Tsinghua/Notebook">NOTEBOOK</a>
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<ul>
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        <li><a href="https://2014.igem.org/Team:Tsinghua/Notebook">OVERVIEW</a></li>
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        <li><a href="https://2014.igem.org/Team:Tsinghua/Notebook/Protocol">PROTOCOL</a></li>
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        <li><a href="https://2014.igem.org/Team:Tsinghua/Notebook/Lablog">LABLOG</a></li>
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       <li><a href="#">HUMAN PRACTICE</a></li>
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       <li><a href="#">TEAM</a></li>
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       <li><a href="https://2014.igem.org/Team:Tsinghua/Human_practice">HUMAN PRACTICE</a>
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  <li><a href="https://2014.igem.org/Team:Tsinghua/Human_practice">OVERVIEW</a></li>
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          <li><a href="https://2014.igem.org/Team:Tsinghua/Human_practice/Gallery">GALLERY</a></li>
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          <li><a href="https://2014.igem.org/Team:Tsinghua/Human_practice/Outreach">OUTREACH</a></li>
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          <li><a href="https://2014.igem.org/Team:Tsinghua/Human_practice/Video">VIDEO</a></li>         
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       <li><a href="https://2014.igem.org/Team:Tsinghua/Team">TEAM</a></li>
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     <h1>Introduction</h1>
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     <h1>Introduction: Overview</h1>
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    <h2>Diabetes Mellitus Type I</h2>
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<h2>Abstract</h2>
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      <p>Diabetes mellitus is a chronic disease involving excessively high levels of blood glucose, either due to absence of insulin production (Type I), or decreased insulin production or insulin receptor dysfunction (Type II). Diabetes can be fatal if left untreated. A study estimates 366 million people are affected by diabetes worldwide, and predicts the number to rise to 552 million by the end of 2030<sup>[1]</sup>.</p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;<i><b> Type I diabetes mellitus </b></i> (T1DM) affects more than 17 million people worldwide, and current treatments for T1DM, known as <i><b> insulin therapy </b></i>, require continued insulin injection, diet control, and constant monitoring of blood sugar. <i><b> Gene therapy </b></i> methods that restore insulin production in non-pancreatic cells might provide a <i><b> one-shot cure </b></i> for T1DB. We propose a gene therapy for T1DM using an <i><b> adeno-associated viral </b></i> (AAV) vector that transfects somatic cells with an insulin gene controlled by a glucose-sensitive promoter, thus potentially restoring <i><b> glucose-regulated </b></i> insulin production in diabetic patients. Preliminary tests are conducted on cell lines to assess the efficiency of the therapy.</p>
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  <p>Type I diabetes mellitus (T1DM) account for 5 % – 10 %<sup> [3, 4]</sup> of diabetes cases. Nonetheless, a cure or prevention for T1DM does not yet exist. Type I diabetes can occur anytime in life, and is most prevalent in children and teenagers aged 8 – 16. The causes for type I diabetes remains unclear, it is, however, most commonly attributed to autoimmune destruction of insulin-producing pancreatic beta cells.</p>
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  <p><a href="https://static.igem.org/mediawiki/2014/1/19/Tsinghua_intro_fig_1.jpg"><img src="https://static.igem.org/mediawiki/2014/1/19/Tsinghua_intro_fig_1.jpg" alt="" width=480 height=249 class="center"></a></p>
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<section><p>&nbsp;</p></section>
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  <p>Figure 1. An estimation of type I diabetes global incidence by region. Data shown are cases per 100,000 people. (Adapted from [2])</p>
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  <p>Currently, the primary treatment for T1DM is insulin therapy, which involves daily injections of insulin and constant monitoring of blood glucose levels as well as food intake. Insulin pumps and continuous blood glucose monitors are increasingly utilized to ameliorate the need for constant care. However, insulin therapy requires regular, if not constant, maintenance, and is by no means considered a cure.</p>
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  <p>There are attempts at developing a cure for T1DM, either through islet transplantation or stem cell regeneration, which would restore insulin production in T1DM patients. A more novel approach involves gene therapy, aimed at granting somatic cells other than beta cells the ability to produce insulin via artificial gene transfer.</p>
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<h2>Background</h2>
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  <p>&nbsp;</p>
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<p>Interested? Click on any of the icons below to get more information on Diabetes and Gene Therapy.</p>
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<div class="iconContainer_200">
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<div class="iconLeft_200" id="intro_diabetes" onclick="location.href='https://2014.igem.org/Team:Tsinghua/Introduction/Diabetes'"></div>
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<div class="iconRight_200" id="intro_genetherapy" onclick="location.href='https://2014.igem.org/Team:Tsinghua/Introduction/Gene_therapy'"></div>
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</section>
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<section>
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<h2>Achievements</h2>
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<h3>Bronze Medal:</h3>
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<ul>
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<li>We have registered our team, have a great summer, and plan to have fun at the Giant Jamboree.</li>
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<li>We have successfully completed and submitted the iGEM 2014 Judging form.</li>
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<li>We have created and shared a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</li>
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<li>We have already planned to present a Poster and Talk at the iGEM Jamboree.</li>
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<li>We have designed and built four standard biobricks in our project,
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<ul>
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<li><a href="http://parts.igem.org/Part:BBa_K1328000">BBa_K1328000</a></li><li><a href="http://parts.igem.org/Part:BBa_K1328002">BBa_K1328002</a></li><li><a href="http://parts.igem.org/Part:BBa_K1328003">BBa_K1328003</a></li><li><a href="http://parts.igem.org/Part:BBa_K1328004">BBa_K1328004</a></li>
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</ul>
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</li>
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</ul>
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<h3>Silver Medal:</h3>
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<ul>
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<li>Experimentally validate that more than one new BioBrick Part of our own design and construction works as expected.</li>
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<li>We have documented the characterization of all the part in the Main Page section of that Part's/Device's Registry entry.</li>
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 +
<li>We have submitted our four new parts to the iGEM Parts Registry.</li>
 +
 
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<li>Beyond the bench, we did the team communication, talked to young students, reached out to Fellow Scholars and made a movie explaining the good and bad of the virus.</li>
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</ul>
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<h3>Gold Medal:</h3>
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<ul>
 +
 
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<li>We assisted Tsinghua-A in preparing their parts for submission and helping them with shipping.</li>
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<li>We provided consultation to BNU-China when they started their first team. </li>
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<li>We sent 8 of our parts from our team DNA repository to NUDT-China team to help them start their experiments. Some of these parts were created by Team Tsinghua for iGEM 2013 and others were from the DNA distribution kit from 2013.</li>
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</ul>
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<h3>Policy and Practices</h3>
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<p><a href="https://2014.igem.org/Team:Tsinghua/Human_practice">Click here</a> for a link to our Policy Practices page (Human Practices)</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:Tsinghua/Human_practice/Gallery">The Future of Synthetic Biology</a></li>
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<li><a href="https://2014.igem.org/Team:Tsinghua/Human_practice/Outreach">Reaching out to Fellow Scholars</a></li>
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<li><a href="https://2014.igem.org/Team:Tsinghua/Human_practice/Video">"Virus" - the Short Movie</a></li>
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      <h3>Reference</h3>
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      <p>[1] Whiting, David R., et al.  &quot;IDF diabetes atlas: global estimates of the prevalence of diabetes for 2011  and 2030.&quot;&nbsp;<em>Diabetes  research and clinical practice</em>&nbsp;94.3  (2011): 311-321.<br>
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[2] Mark A Atkinson, George S  Eisenbarth, Aaron W Michels, Type 1 diabetes, The Lancet, Volume 383, Issue  9911, 4–10 January 2014, Pages 69-82, ISSN 0140-6736, http://dx.doi.org/10.1016/S0140-6736(13)60591-7.<br>
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[3]  Type 1 diabetes, American Diabetes Association. <a href="http://www.diabetes.org/diabetes-basics/type-1/">http://www.diabetes.org/diabetes-basics/type-1/</a> <br>
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[4]  Type 1 diabetes, NHS. <a href="http://www.nhs.uk/conditions/Diabetes-type1/Pages/Introduction.aspx">http://www.nhs.uk/conditions/Diabetes-type1/Pages/Introduction.aspx</a></p>
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Latest revision as of 23:25, 17 October 2014

Team Tsinghua 2014 Return to iGEM 2014

Introduction: Overview

Abstract

     Type I diabetes mellitus (T1DM) affects more than 17 million people worldwide, and current treatments for T1DM, known as insulin therapy , require continued insulin injection, diet control, and constant monitoring of blood sugar. Gene therapy methods that restore insulin production in non-pancreatic cells might provide a one-shot cure for T1DB. We propose a gene therapy for T1DM using an adeno-associated viral (AAV) vector that transfects somatic cells with an insulin gene controlled by a glucose-sensitive promoter, thus potentially restoring glucose-regulated insulin production in diabetic patients. Preliminary tests are conducted on cell lines to assess the efficiency of the therapy.

 

Background

Interested? Click on any of the icons below to get more information on Diabetes and Gene Therapy.

Achievements

Bronze Medal:

  • We have registered our team, have a great summer, and plan to have fun at the Giant Jamboree.
  • We have successfully completed and submitted the iGEM 2014 Judging form.
  • We have created and shared a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.
  • We have already planned to present a Poster and Talk at the iGEM Jamboree.
  • We have designed and built four standard biobricks in our project,

Silver Medal:

  • Experimentally validate that more than one new BioBrick Part of our own design and construction works as expected.
  • We have documented the characterization of all the part in the Main Page section of that Part's/Device's Registry entry.
  • We have submitted our four new parts to the iGEM Parts Registry.
  • Beyond the bench, we did the team communication, talked to young students, reached out to Fellow Scholars and made a movie explaining the good and bad of the virus.

Gold Medal:

  • We assisted Tsinghua-A in preparing their parts for submission and helping them with shipping.
  • We provided consultation to BNU-China when they started their first team.
  • We sent 8 of our parts from our team DNA repository to NUDT-China team to help them start their experiments. Some of these parts were created by Team Tsinghua for iGEM 2013 and others were from the DNA distribution kit from 2013.

Policy and Practices

Click here for a link to our Policy Practices page (Human Practices)

Retrieved from "http://2014.igem.org/Team:Tsinghua/Introduction"