Team:Bordeaux/NotebookJune13

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<div class="type-exp"> Molecular biology</div>
<div class="type-exp"> Molecular biology</div>

Latest revision as of 22:43, 17 October 2014

13th June

Molecular biology

  • Digestion, ligation and transformation of pSB6A1 and our PCR products VPGXG-20, VPGXG-40 and VPGXG-60.
    • Transformation by electroporation in BL21


Purification

  • End of purification protocol and store at 4°C


Design

  • Research in bibliography on consensus sequences of dragline silk
    • With this sequences, we would like to synthetize our Silk-Like-Polypeptide sequence from our own oligos (based on Gibson assembly protocol)

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