Team:EPF Lausanne/Attributions

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<h1>Acknowledgements</h1>
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              <h1 class="cntr">ATTRIBUTIONS</h1>
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<h2>General support</h2>
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<p>The project's experiments were all conducted by the student members of the 2014 iGEM EPFL <a target="_blank" href="https://2014.igem.org/Team:EPF_Lausanne/Team" id="team">team</a>. To better tackle each part of the project, we divided the team in small groups. These groups evolved throughout the project depending of the needs for each experiment.<!-- and the stage of each sub-division.--></p>
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<p>Mrs Marie-France Radigois helped us with the administrative part of the project.</p>
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<p>Mr André Pexieder let us use his beloved lab for our project.</p>
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<li>The design & characterisation of the CpxR stress-sensitive response in <i>E. coli</i> was done by Ted Baldwin, Cécile Piot and Nikolaus Huwiler.</li>
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<p>Mrs Celia Lutherbacher helped us polish the documentation.</p>
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<li>The design & characterisation of the full and split luciferases in <i>E. coli</i> was done by Sakura Nussbaum, Lucie Petetin, Grégoire Repond and Thomas Simonet.</li>
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<li>The design & characterisation of the arabinose promoter was done by Lucie Petetin and Ione Pla.</li>
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<li>The design & characterisation of the HOG osmo response in <i>S. cerevisiae</i> was done by Romane Breysse, Jin Chang and Bastien Duckert.</li>
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<li>The design, fabrication and tests of the different microfluidic chips were done by Axel de Tonnac, Grégoire Repond and Thomas Simonet.</li>
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<li>The signal detection and processing with the Raspberry Pi was implemented by Arthur Giroux.</li>
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<li>The study of the biosafety issues related to microfluidic chips was done by Axel de Tonnac, Grégoire Repond and Thomas Simonet.</li>
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<li>The human practices part of the project was mainly done by Sakura Nussbaum and Ione Pla.</li>
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<li>The wiki design was done by Arthur Giroux.</li>
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<li>Most of the administrative aspect was done by Ione Pla.</li>
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<li>The entire team contributed to the content of the wiki.</li>
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<h2>Laboratory techniques</h2>
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<p>Mr Arne Seitz and Mr José Artacho taught us how to use the microscopes at EPFL's <a target="_blank" href="http://biop.epfl.ch/">Bioimaging and Optics Facility</a></p>
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<p>Mr Marc Chambon taught us how to use the plate readers at EPFL's <a target="_blank" href="http://bsf.epfl.ch/">Biomolecular Screening Facility</a>.</p>
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<p>Mr Julien Dorsaz and Mr Kaspar Suter trained us on the different clean room processes we needed to know to make the masks and wafers for our microfluidic chips. You can find more information on EPFL's center of micronanotechnology <a target="_blank" href="http://cmi.epfl.ch/">here</a>.</p>
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<p>Ms Luisa Spisak from EPFL 2013 team helped us starting the project by showing us basic lab techniques.</p>
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<h2>Yeast handling and transformation techniques</h2>
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<p><a target="_blank" href="http://simanis-lab.epfl.ch/">Professor Simanis</a> and Mrs Andrea Krapp helped us a lot to begin the yeast part of the project. They clarified many points regarding yeast transformations and gene regulation in S. Cerevisiae. They confirmed the feasibility of our project and validated the constructs we had designed.</p>
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<p>Mr Feuz Jean-Daniel, working in our adivsor <a target="_blank" href="http://deplanckelab.epfl.ch">Professor Bart Deplancke's</a> lab, gave us the ym4271 yeast strain as well as several protocols for yeast selective and unselective medium preparation.
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<h2>Collaboration</h2>
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<p>Professor Stephen Michnick and Jacqueline Kowarzyk sent us their split IFP plasmids, which we extensively used to test CpxR dimerization.</p>
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<p>Dr Stavroula Hatzios, Postdoc in <a target="_blank" href="http://waldorlab.bwh.harvard.edu">Professor Waldor's laboratory</a> sent us her renilla luciferase plasmids with CheY/CheZ, which helped us test the luminescence.</p>
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<p>Mr Yann Heurtaux, Mr Clément Epié, and Mr Nicolas Loubet, are co-founders of biohackerspaces in France and Switzerland. They sought us out and came to visit, leading to presentations about iGEM and our project at the <a target="_blank" href="http://hackuarium.strikingly.com/">Hackuarium</a> in Lausanne.
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<p>Mr Fabien Goubet is a journalist for <a target="_blank" href="http://www.letemps.ch/">"Le Temps"</a> newspaper. He came to visit us at EPFL and wrote an <a target="_blank" href="https://static.igem.org/mediawiki/2014/8/8d/20140927_01_%282%29.jpg">article</a> about our project. </p>
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<p>Mr Yves Deluz, general director of the highschools from Canton de Vaud, sent us 4 classes of high school students for a day of discovery of synthetic biology at EPFL.</p>
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<p>Mrs Françoise Bezençon, Mr Fabio Moriggia, Mr Jean-Pierre Lardet and Mr Nabil Mastour, highschool biology teachers, collaborated with us and came with their students for the visit at EPFL.</p>
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<p>The Teaching Assistants and Professors gave us regular feedback at the weekly meetings throughout the project.</p>
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<p>You can find our Acknowledgements page <a target="_blank" href="https://2014.igem.org/Team:EPF_Lausanne/Acknowledgments">here</a>.</p>
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Latest revision as of 22:32, 17 October 2014

ATTRIBUTIONS


The project's experiments were all conducted by the student members of the 2014 iGEM EPFL team. To better tackle each part of the project, we divided the team in small groups. These groups evolved throughout the project depending of the needs for each experiment.

  • The design & characterisation of the CpxR stress-sensitive response in E. coli was done by Ted Baldwin, Cécile Piot and Nikolaus Huwiler.
  • The design & characterisation of the full and split luciferases in E. coli was done by Sakura Nussbaum, Lucie Petetin, Grégoire Repond and Thomas Simonet.
  • The design & characterisation of the arabinose promoter was done by Lucie Petetin and Ione Pla.
  • The design & characterisation of the HOG osmo response in S. cerevisiae was done by Romane Breysse, Jin Chang and Bastien Duckert.
  • The design, fabrication and tests of the different microfluidic chips were done by Axel de Tonnac, Grégoire Repond and Thomas Simonet.
  • The signal detection and processing with the Raspberry Pi was implemented by Arthur Giroux.
  • The study of the biosafety issues related to microfluidic chips was done by Axel de Tonnac, Grégoire Repond and Thomas Simonet.
  • The human practices part of the project was mainly done by Sakura Nussbaum and Ione Pla.
  • The wiki design was done by Arthur Giroux.
  • Most of the administrative aspect was done by Ione Pla.
  • The entire team contributed to the content of the wiki.

The Teaching Assistants and Professors gave us regular feedback at the weekly meetings throughout the project.

You can find our Acknowledgements page here.

Sponsors

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