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Team:Evry/Notebook/Transformation/09-01-2014
From 2014.igem.org
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<u> <b> Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Selection </b> </u> <br> | <u> <b> Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Selection </b> </u> <br> | ||
| - | + | Plates are showed and organised like shown in the following picture: <br></p> | |
| - | Plates are showed and organised like shown in the following picture: <br> | + | |
<div align="center"><img src="https://static.igem.org/mediawiki/2014/b/bf/PlatesTransfo%282%29.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" /></div> | <div align="center"><img src="https://static.igem.org/mediawiki/2014/b/bf/PlatesTransfo%282%29.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" /></div> | ||
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<td> <b>A1 </b> </td> | <td> <b>A1 </b> </td> | ||
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<td>Pseudo Ø plasmid </td> | <td>Pseudo Ø plasmid </td> | ||
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<td> <b>A4 </b> </td> | <td> <b>A4 </b> </td> | ||
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We also made MB only plates to see the normal growth of our bacteria,which were showed and organised like shown in the following picture: <br> | We also made MB only plates to see the normal growth of our bacteria,which were showed and organised like shown in the following picture: <br> | ||
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<div align="center"><img src="https://static.igem.org/mediawiki/2014/1/1e/PlatesTransfoMBonly.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" /></div> <br> | <div align="center"><img src="https://static.igem.org/mediawiki/2014/1/1e/PlatesTransfoMBonly.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" /></div> <br> | ||
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<table border="1"> | <table border="1"> | ||
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Latest revision as of 22:12, 17 October 2014
Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Transformation
| pBBR1MCS | pRhokHI-2 | Ø plasmid |
| Pseudo replicate 1 | Pseudo replicate 1 | Pseudo |
| Pseudo replicate 2 | Pseudo replicate 2 | E.Coli Bl21 |
| E.Coli Bl21 | E.Coli Bl21 |
Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Selection
Plates are showed and organised like shown in the following picture:
| A1 | B1 | C1 | D1 |
| Pseudo+pBBR1MCS (1) | Pseudo+pBBR1MCS (2) | Pseudo | Pseudo Ø plasmid |
| A2 | B2 | C2 | D2 |
| Pseudo+pRhokHI-2 (1) | Pseudo+pRhokHI-2 (2) | Pseudo | Pseudo Ø plasmid |
| A3 | B3 | C3 |
| E.Coli Bl21 | Bl21 Ø plasmid | Bl21+pRhokHI-2 |
| A4 | B4 | C4 |
| E.Coli Bl21 | Bl21 Ø plasmid | Bl21+pBBR1MCS |
We also made MB only plates to see the normal growth of our bacteria,which were showed and organised like shown in the following picture:
| A | B | C | D | a | b | c | d | e | f |
| E.Coli Bl21 | Bl21 Ø plasmid | Bl21+pBBR1MCS | Bl21+pRhokHI-2 | Pseudo | Pseudo Ø plasmid | Pseudo+pRhokHI-2 (1) | Pseudo+pRhokHI-2 (2) | Pseudo+pBBR1MCS (1) | Pseudo+pBBR1MCS (2) |
Sep 01
