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Team:Evry/Notebook/Transformation/09-01-2014
From 2014.igem.org
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<u> <b> Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Transformation </b> </u> <br> | <u> <b> Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Transformation </b> </u> <br> | ||
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<table border="1"> | <table border="1"> | ||
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</table> | </table> | ||
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| - | + | <p> | |
<u> <b> Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Selection </b> </u> <br> | <u> <b> Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Selection </b> </u> <br> | ||
| - | + | Plates are showed and organised like shown in the following picture: <br></p> | |
| - | Plates are showed and organised like shown in the following picture: <br> | + | <div align="center"><img src="https://static.igem.org/mediawiki/2014/b/bf/PlatesTransfo%282%29.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" /></div> |
| - | <img src="https://static.igem.org/mediawiki/2014/ | + | <div style="float:left;"> |
| - | + | <table border="1" > | |
| - | <table border="1"> | + | |
<tr > | <tr > | ||
<td> <b>A1 </b> </td> | <td> <b>A1 </b> </td> | ||
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<td>Pseudo Ø plasmid </td> | <td>Pseudo Ø plasmid </td> | ||
</tr> | </tr> | ||
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</table> | </table> | ||
| - | < | + | </div> |
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| - | <table border="1"> | + | <table border="1" > |
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<td> <b>A4 </b> </td> | <td> <b>A4 </b> </td> | ||
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| - | </table> | + | </table></div> |
| - | <br> | + | <br><br> |
| + | <br><div align="center"> | ||
| + | <p> | ||
| + | We also made MB only plates to see the normal growth of our bacteria,which were showed and organised like shown in the following picture: <br> | ||
| + | </p> | ||
| + | <div align="center"><img src="https://static.igem.org/mediawiki/2014/1/1e/PlatesTransfoMBonly.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" /></div> <br> | ||
| + | </div> | ||
| + | <table border="1"> | ||
| + | <tr > | ||
| + | <td> <b>A </b> </td> | ||
| + | <td> <b>B </b> </td> | ||
| + | <td> <b>C </b> </td> | ||
| + | <td> <b>D </b> </td> | ||
| + | <td> <b>a </b> </td> | ||
| + | <td> <b>b </b> </td> | ||
| + | <td> <b>c </b> </td> | ||
| + | <td> <b>d </b> </td> | ||
| + | <td> <b>e </b> </td> | ||
| + | <td> <b>f </b> </td> | ||
| + | </tr> | ||
| - | + | <tr > | |
| - | + | <td>E.Coli Bl21 </td> | |
| - | < | + | <td>Bl21 Ø plasmid </td> |
| + | <td>Bl21+pBBR1MCS </td> | ||
| + | <td>Bl21+pRhokHI-2 </td> | ||
| + | <td>Pseudo </td> | ||
| + | <td>Pseudo Ø plasmid </td> | ||
| + | <td>Pseudo+pRhokHI-2 (1)</td> | ||
| + | <td>Pseudo+pRhokHI-2 (2)</td> | ||
| + | <td>Pseudo+pBBR1MCS (1)</td> | ||
| + | <td>Pseudo+pBBR1MCS (2)</td> | ||
| + | </tr> | ||
| + | <br> | ||
| + | </table> | ||
| + | <br> | ||
</p> | </p> | ||
| - | <span class="cd-date"> | + | <span class="cd-date">Sep 01</span> |
</div> | </div> | ||
</div> | </div> | ||
</html> | </html> | ||
Latest revision as of 22:12, 17 October 2014
Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Transformation
| pBBR1MCS | pRhokHI-2 | Ø plasmid |
| Pseudo replicate 1 | Pseudo replicate 1 | Pseudo |
| Pseudo replicate 2 | Pseudo replicate 2 | E.Coli Bl21 |
| E.Coli Bl21 | E.Coli Bl21 |
Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Selection
Plates are showed and organised like shown in the following picture:
| A1 | B1 | C1 | D1 |
| Pseudo+pBBR1MCS (1) | Pseudo+pBBR1MCS (2) | Pseudo | Pseudo Ø plasmid |
| A2 | B2 | C2 | D2 |
| Pseudo+pRhokHI-2 (1) | Pseudo+pRhokHI-2 (2) | Pseudo | Pseudo Ø plasmid |
| A3 | B3 | C3 |
| E.Coli Bl21 | Bl21 Ø plasmid | Bl21+pRhokHI-2 |
| A4 | B4 | C4 |
| E.Coli Bl21 | Bl21 Ø plasmid | Bl21+pBBR1MCS |
We also made MB only plates to see the normal growth of our bacteria,which were showed and organised like shown in the following picture:
| A | B | C | D | a | b | c | d | e | f |
| E.Coli Bl21 | Bl21 Ø plasmid | Bl21+pBBR1MCS | Bl21+pRhokHI-2 | Pseudo | Pseudo Ø plasmid | Pseudo+pRhokHI-2 (1) | Pseudo+pRhokHI-2 (2) | Pseudo+pBBR1MCS (1) | Pseudo+pBBR1MCS (2) |
Sep 01
