Team:Evry/Notebook/Transformation/09-01-2014

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<u> <b> Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Transformation </b> </u> <br>
<u> <b> Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Transformation </b> </u> <br>
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<u> <b> Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Selection </b> </u> <br>
<u> <b> Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Selection </b> </u> <br>
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Plates are showed and organised like shown in the following picture: <br></p>
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<div align="center"><img src="https://static.igem.org/mediawiki/2014/b/bf/PlatesTransfo%282%29.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" /></div>
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<div style="float:left;">
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<table border="1" >
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<tr >
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  <td> <b>A1 </b> </td>
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  <td> <b>B1 </b> </td>
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  <td> <b>C1 </b> </td>
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  <td> <b>D1 </b> </td>
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</tr>
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<tr >
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  <td>Pseudo+pBBR1MCS (1)</td>
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  <td>Pseudo+pBBR1MCS (2)</td>
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  <td>Pseudo </td>
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  <td>Pseudo Ø plasmid </td>
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</tr>
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</table>
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</div>
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<div style="float:right;">
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<table border="1">
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<tr >
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  <td> <b>A2 </b> </td>
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  <td> <b>B2 </b> </td>
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  <td> <b>C2 </b> </td>
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  <td> <b>D2 </b> </td>
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</tr>
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<tr >
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  <td>Pseudo+pRhokHI-2 (1)</td>
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  <td>Pseudo+pRhokHI-2 (2)</td>
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  <td>Pseudo </td>
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  <td>Pseudo Ø plasmid </td>
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</tr>
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</table>
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</div>
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<br><br><br>
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<div style="float:left;">
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<table border="1">
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<tr >
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  <td> <b>A3 </b> </td>
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  <td> <b>B3 </b> </td>
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  <td> <b>C3 </b> </td>
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</tr>
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<tr >
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  <td>E.Coli Bl21 </td>
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  <td>Bl21 Ø plasmid </td>
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  <td>Bl21+pRhokHI-2 </td>
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</tr>
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</table>
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<br>
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</div>
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<div style="float:right;">
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<table border="1" >
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<tr >
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  <td> <b>A4 </b> </td>
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  <td> <b>B4 </b> </td>
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  <td> <b>C4 </b> </td>
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</tr>
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 +
<tr >
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  <td>E.Coli Bl21 </td>
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  <td>Bl21 Ø plasmid </td>
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  <td>Bl21+pBBR1MCS </td>
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</tr>
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</table></div>
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<br><br>
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<br><div align="center">
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<p>
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We also made MB only plates to see the normal growth of our bacteria,which were showed and organised like shown in the following picture: <br>
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</p>
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<div align="center"><img src="https://static.igem.org/mediawiki/2014/1/1e/PlatesTransfoMBonly.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" /></div> <br>
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</div>
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<table border="1">
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<tr >
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  <td> <b>A </b> </td>
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  <td> <b>B </b> </td>
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  <td> <b>C </b> </td>
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  <td> <b>D </b> </td>
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  <td> <b>a </b> </td>
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  <td> <b>b </b> </td>
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  <td> <b>c </b> </td>
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  <td> <b>d </b> </td>
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  <td> <b>e </b> </td>
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  <td> <b>f </b> </td>
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</tr>
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 +
<tr >
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  <td>E.Coli Bl21 </td>
 +
  <td>Bl21 Ø plasmid </td>
 +
  <td>Bl21+pBBR1MCS </td>
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  <td>Bl21+pRhokHI-2 </td>
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  <td>Pseudo </td>
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  <td>Pseudo Ø plasmid </td>
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  <td>Pseudo+pRhokHI-2 (1)</td>
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  <td>Pseudo+pRhokHI-2 (2)</td>
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  <td>Pseudo+pBBR1MCS (1)</td>
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  <td>Pseudo+pBBR1MCS (2)</td>
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 +
</tr>
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<br>
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</table>
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<br>
</p>
</p>
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<span class="cd-date">Aug 24</span>  
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<span class="cd-date">Sep 01</span>  
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Latest revision as of 22:12, 17 October 2014

Picture

Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Transformation

pBBR1MCS pRhokHI-2 Ø plasmid
Pseudo replicate 1 Pseudo replicate 1 Pseudo
Pseudo replicate 2 Pseudo replicate 2 E.Coli Bl21
E.Coli Bl21 E.Coli Bl21

Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Selection
Plates are showed and organised like shown in the following picture:

GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE
A1 B1 C1 D1
Pseudo+pBBR1MCS (1) Pseudo+pBBR1MCS (2) Pseudo Pseudo Ø plasmid
A2 B2 C2 D2
Pseudo+pRhokHI-2 (1) Pseudo+pRhokHI-2 (2) Pseudo Pseudo Ø plasmid



A3 B3 C3
E.Coli Bl21 Bl21 Ø plasmid Bl21+pRhokHI-2

A4 B4 C4
E.Coli Bl21 Bl21 Ø plasmid Bl21+pBBR1MCS



We also made MB only plates to see the normal growth of our bacteria,which were showed and organised like shown in the following picture:

GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE


A B C D a b c d e f
E.Coli Bl21 Bl21 Ø plasmid Bl21+pBBR1MCS Bl21+pRhokHI-2 Pseudo Pseudo Ø plasmid Pseudo+pRhokHI-2 (1) Pseudo+pRhokHI-2 (2) Pseudo+pBBR1MCS (1) Pseudo+pBBR1MCS (2)

Sep 01

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