Team:Caltech/week5
From 2014.igem.org
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<ul><li>Grew liquid cultures of <i>E. coli</i> transformed with pWW2149+pWW1521 and pWW2149+pWW1523 (colonies had been allowed to grow on agar plates over the weekend)</li> | <ul><li>Grew liquid cultures of <i>E. coli</i> transformed with pWW2149+pWW1521 and pWW2149+pWW1523 (colonies had been allowed to grow on agar plates over the weekend)</li> | ||
</ul> | </ul> | ||
- | <b> | + | <b>Combinatorial Promoters</b> |
- | <ul><li></li> | + | <ul><li>Set up overnight liquid cultures of DH5α-Z1 transformed with pAA001 (with pTet-pLac combinatorial promoter)</li> |
+ | <li>Transformed DH5α-Z1 with Gibson product (aimed at assembling pAA003--containing pBAD-pTet combinatorial promoter) created Friday.</li> | ||
</ul> | </ul> | ||
</td> | </td> | ||
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</ul> | </ul> | ||
<b>agrBCDA Reception System and Combinatorial Promoters</b> | <b>agrBCDA Reception System and Combinatorial Promoters</b> | ||
- | <ul> | + | <ul><li>Began experiments on bacteria transformed with pAA001, testing different inducer concentrations: |
+ | <ul><li>([IPTG] in μM, [aTc] in ng/mL): (0,0); (0,100); (100,0); (100,100)</li> | ||
+ | <li> Preliminary results promising: GFP <a href="2014.igem.org/Team:Caltech/Results">significantly expressed</a> [include link to the graph] only at 100 μM IPTG, 100 ng/mL aTc. </li> | ||
+ | </ul></li> | ||
+ | <li>Began assembly of agr reception system: | ||
+ | <ul><li> | ||
</ul> | </ul> | ||
</td> | </td> |
Revision as of 18:34, 16 July 2014
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