Team:Hong Kong HKUST/wetlab

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        <li><a href="#">Team</a>
 
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<h3 style="text-align:center">Wetlab</h3>
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<h2>Protocol</h2>
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<p>The wet lab started at the beginning of summer and lasted for three months. Our wet lab work is divided into four parts, namely:
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<p>We have a variety of protocols that we followed for working on our project. The step-by-step procedures of each technique
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(1) (Detection Module) ComD & ComE, (2) (Detection Module) P<sub><i>comCDE</i></sub>, (3) (&sigma;<sup>X</sup> promoters Module) &sigma;<sup>X</sup> & ComW, and
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  can be found here!
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(4) (&sigma;<sup>X</sup> promoters Module) P<sub>celA</sub> and P<sub>comFA</sub>. During the summer, lab work such as primer design, digestion, and ligation was carried out extensively
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and useful data were collected. These can be retrieved from Notebook and Protocols pages for details of experimental flows.
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The BioBrick, researchers, environmental and university safety issues can be found in Safety page. In terms of project process,
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some experiments could not be completed due to time constrains and they are outlined in Future Work page.
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<h4> Mutagenesis</h4>
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<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
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visibly touching the cheekbone below the eye. </p>
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<h4> Western Blot </h4>
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<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
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Notebook
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visibly touching the cheekbone below the eye. </p>
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Protocols
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visibly touching the cheekbone below the eye. </p>
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<h4> Ligation </h4>
 
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<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
 
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visibly touching the cheekbone below the eye. </p>
 
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<h4> Ethanol Precipitation</h4>
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<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
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Safety
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visibly touching the cheekbone below the eye. </p>
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<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
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visibly touching the cheekbone below the eye. </p>
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<h4> Gibson Assembly </h4>
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<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
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visibly touching the cheekbone below the eye. </p>
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<h4> Polymerase Chain Reaction (PCR)</h4>
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<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
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visibly touching the cheekbone below the eye. </p>
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<li class='site_link'><a href="#">Medal Requirements</li></a>
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Latest revision as of 22:01, 17 October 2014



Wetlab

The wet lab started at the beginning of summer and lasted for three months. Our wet lab work is divided into four parts, namely: (1) (Detection Module) ComD & ComE, (2) (Detection Module) PcomCDE, (3) (σX promoters Module) σX & ComW, and (4) (σX promoters Module) PcelA and PcomFA. During the summer, lab work such as primer design, digestion, and ligation was carried out extensively and useful data were collected. These can be retrieved from Notebook and Protocols pages for details of experimental flows. The BioBrick, researchers, environmental and university safety issues can be found in Safety page. In terms of project process, some experiments could not be completed due to time constrains and they are outlined in Future Work page.


Home

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Riboregulator

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WetLab

Achievement