Team:Berlin/Project

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    <a href="https://2014.igem.org/Team:Berlin" class="main-menue-links"><li>Home</li></a>
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    <a href="https://2014.igem.org/Team:Berlin/Project" class="main-menue-links"><li class="active">Project</li></a>
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    <a href="https://2014.igem.org/Team:Berlin/Team" class="main-menue-links"><li>Team</li></a>
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    <a href="https://igem.org/Main_Page" class="igem-link"><li><img class="igem-logo" src="https://static.igem.org/mediawiki/2014/d/d8/Team_Berlin_igem_logo.png"></li></a>
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  <a class="navmenu-brand" href="https://2014.igem.org/Team:Berlin"><h1>iGEM Berlin</h1></a>
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<h1 >WELCOME TO iGEM Berlin 2014! </h1>
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<p>Hello everyone this is the preliminary wiki of iGEM Berlin. We are working on magnetic E. coli and if you would like to know why and how we do that just visit our homepage.
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    <li><a href="https://2014.igem.org/Team:Berlin" class="active">Home</a></li>
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<br>Visit http://www.igem.berlin </p>
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    <li><a href="https://2014.igem.org/Team:Berlin/Project">Project</a></li>
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<br>
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    <li><a href="https://2014.igem.org/Team:Berlin/Team">Team</a></li>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Berlin/Project&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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    <li><a href="https://2014.igem.org/Team:Berlin/Safety">Safety</a></li>
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    <li><a href="https://2014.igem.org/Team:Berlin/Workshop">Workshop</a></li>
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    <li><a href="https://2014.igem.org/Team:Berlin/Blog">Blog</a></li>
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    <li><a href="https://igem.org/Main_Page" class="igem-link"><img class="igem-logo" src="https://static.igem.org/mediawiki/2014/d/d8/Team_Berlin_igem_logo.png"></a></li>
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<a href="https://2014.igem.org/Team:Berlin"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:Berlin/Team"style="color:#000000"> Team </a> </td>
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        <h2><img src="https://static.igem.org/mediawiki/2014/f/ff/Team_Berlin_igem_questionmark.png" alt="" class="teaser-icons hidden-xs" />Explore our Project:</h2>
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        <!--<a href="#" class="more-informations-white">More informations</a>-->
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<!--- ////////////////// DESCRIPTION////////////////// -->
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Berlin"style="color:#000000"> Official Team Profile </a></td>
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              <a href="https://2014.igem.org/Team:Berlin/Project" class="sub-link-project"> 1. What is it all about?</a><br/><br/>
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            <a href="https://2014.igem.org/Team:Berlin/Project/Activities" class="sub-link-project"> 2. Project-related Activities</a><br/><br/>
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              <a href="https://2014.igem.org/Team:Berlin/Project/Detailed-Description" class="sub-link-project">3. Detailed Description</a><br/><br/>
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              <a href="https://2014.igem.org/Team:Berlin/Project/Results" class="sub-link-project">4. Our Results</a><br/><br/>
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              <a href="https://2014.igem.org/Team:Berlin/Project/Summary" class="sub-link-project">5. Lab Summary</a><br/><br/>
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              <a href="https://2014.igem.org/Team:Berlin/Project/Journal" class="sub-link-project">6. Lab Journal</a><br/><br/>
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              <a href="https://2014.igem.org/Team:Berlin/Project/Property" class="sub-link-project">7. Intellectual Property</a><br/><br/>
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<a href="https://2014.igem.org/Team:Berlin/Project"style="color:#000000"> Project</a></td>
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      <div class="project-number">1</div><div class="project-headline-float"><h2 class="green-text project-headline"> What is it all about?</h2></div>
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<a href="https://2014.igem.org/Team:Berlin/Parts"style="color:#000000"> Parts</a></td>
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        <a name="description">&nbsp;</a>
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          <strong>iGEM Berlin 2014: A remote control for E. coli</strong><br/>
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<p>
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As the first iGEM team from Berlin, we decided to construct a simple BioBrick that enables synthetic biologists to remotely control the movement of E. coli. A seemingly simple and non-invasive mechanism for this remote control is the use of magnetic fields. By altering the iron homeostasis of E. coli, we want to increase the total iron level of the cytosol. By sequestering iron in a ferritin protein, iron crystals are formed and the cell is detoxified. We also worked with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to create various metal nanoparticles as an alternative strategy. Furthermore, we searched for the optimal conditions which yielded the most efficient formation of magnetic nanoparticles in E. coli. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used to remote control the cellular movement of E. coli.</p><br/>
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          The following animation visualizes the concept of using ferritin iron storage proteins as a magnetism mediating module.<br/>
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          <br/>
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<iframe src="//player.vimeo.com/video/109249906?byline=0&amp;portrait=0&amp;color=0ecd28" width="700" height="394" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe>
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          <br/>
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          <br/>
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          This animation was developed by Florian Renner who is an science interested and very talented graphic designer based in Munich. We thank Florian for his amazing job he did voluntarily for iGEM Berlin helping us to overcome the limitations of our budget.
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          <br/><br/>
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<a href="https://2014.igem.org/Team:Berlin/Modeling"style="color:#000000"> Modeling</a></td>
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<a href="https://2014.igem.org/Team:Berlin/Notebook"style="color:#000000"> Notebook</a></td>
 
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<a href="https://2014.igem.org/Team:Berlin/Safety"style=" color:#000000"> Safety </a></td>
 
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<a href="https://2014.igem.org/Team:Berlin/Attributions"style="color:#000000"> Attributions </a></td>
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      <h3>Sponsors</h3>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
 
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      <img src="https://static.igem.org/mediawiki/2014/0/0e/Team_Berlin_biocat_logo.png" class="img-responsive grayscale">
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      <img src="https://static.igem.org/mediawiki/2014/9/90/Team_Berlin_adares_logo.png" class="img-responsive grayscale">
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      <img src="https://static.igem.org/mediawiki/2014/4/48/Team_Berlin_ibb_logo.png" class="img-responsive grayscale">
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<tr><td > <h3> Project Description </h3></td>
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<td > <h3> Content</h3></td>
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      <img src="https://static.igem.org/mediawiki/2014/8/86/Team_Berlin_roth_logo.png" class="img-responsive grayscale">
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      <img src="https://static.igem.org/mediawiki/2014/4/4a/Team_Berlin_BioLabs_logo.png" class="img-responsive grayscale">
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<p>Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs) </p>
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<br>
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<h3>References </h3>
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      <img src="https://static.igem.org/mediawiki/2014/c/c1/Team_Berlin_Promega_logo.png" class="img-responsive grayscale">
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iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you. </p>  
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      <img src="https://static.igem.org/mediawiki/2014/7/71/Team_Berlin_Agrarboerse_logo.png" class="img-responsive grayscale">
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    <div class="col-sm-2 col-sm-offset-1 col-xs-3">
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      <img src="https://static.igem.org/mediawiki/2014/9/99/Team_Berlin_GenExpress_logo.png" class="img-responsive grayscale">
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      <img src="https://static.igem.org/mediawiki/2014/9/9b/Team_Berlin_era-synbio_logo.png" class="img-responsive grayscale">
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<p> You can use these subtopics to further explain your project</p>
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      <img src="https://static.igem.org/mediawiki/2014/b/b1/Team_Berlin_unicat_logo.jpg" class="img-responsive grayscale">
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      <img src="https://static.igem.org/mediawiki/2014/7/79/Team_Berlin_tuberlin_logo.png" class="img-responsive grayscale">
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    </div>
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    <div class="col-sm-2 col-sm-offset-1 col-xs-3">
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<li>Overall project summary</li>
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      <img src="https://static.igem.org/mediawiki/parts/c/cb/Team_Berlin_florian_renner_logo.png" class="img-responsive grayscale">
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<li>Project Details</li>
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<li>Materials and Methods</li>
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<li>The Experiments</li>
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<li>Results</li>
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<li>Data analysis</li>
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</section>
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<li>Conclusions</li>
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<p>
 
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It's important for teams to describe all the creativity that goes into an iGEM project, along with all the great ideas your team will come up with over the course of your work.
 
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</p>
 
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It's also important to clearly describe your achievements so that judges will know what you tried to do and where you succeeded. Please write your project page such that what you achieved is easy to distinguish from what you attempted.
 
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Latest revision as of 21:59, 17 October 2014

Explore our Project:

1

What is it all about?

  iGEM Berlin 2014: A remote control for E. coli

As the first iGEM team from Berlin, we decided to construct a simple BioBrick that enables synthetic biologists to remotely control the movement of E. coli. A seemingly simple and non-invasive mechanism for this remote control is the use of magnetic fields. By altering the iron homeostasis of E. coli, we want to increase the total iron level of the cytosol. By sequestering iron in a ferritin protein, iron crystals are formed and the cell is detoxified. We also worked with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to create various metal nanoparticles as an alternative strategy. Furthermore, we searched for the optimal conditions which yielded the most efficient formation of magnetic nanoparticles in E. coli. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used to remote control the cellular movement of E. coli.


The following animation visualizes the concept of using ferritin iron storage proteins as a magnetism mediating module.



This animation was developed by Florian Renner who is an science interested and very talented graphic designer based in Munich. We thank Florian for his amazing job he did voluntarily for iGEM Berlin helping us to overcome the limitations of our budget.