Team:Berlin/Project

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   <ul class="main-menue-ul">
   <ul class="main-menue-ul">
     <a href="https://2014.igem.org/Team:Berlin" class="main-menue-links"><li>Home</li></a>
     <a href="https://2014.igem.org/Team:Berlin" class="main-menue-links"><li>Home</li></a>
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     <a href="https://2014.igem.org/Team:Berlin/Project" class="main-menue-links"><li>Project</li></a>
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     <a href="https://2014.igem.org/Team:Berlin/Project" class="main-menue-links"><li class="active">Project</li></a>
     <a href="https://2014.igem.org/Team:Berlin/Team" class="main-menue-links"><li>Team</li></a>
     <a href="https://2014.igem.org/Team:Berlin/Team" class="main-menue-links"><li>Team</li></a>
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     <a href="https://2014.igem.org/Team:Berlin/Safety" class="main-menue-links"><li>Safety</li></a>      
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     <a href="https://2014.igem.org/Team:Berlin/Safety" class="main-menue-links"><li>Safety</li></a>
     <a href="https://2014.igem.org/Team:Berlin/Workshop" class="main-menue-links"><li>Workshop</li></a>
     <a href="https://2014.igem.org/Team:Berlin/Workshop" class="main-menue-links"><li>Workshop</li></a>
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     <a href="https://2014.igem.org/Team:Berlin/Blog"><li>Blog</li></a>
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     <a href="https://2014.igem.org/Team:Berlin/Blog" class="main-menue-links"><li>Blog</li></a>
     <a href="https://igem.org/Main_Page" class="igem-link"><li><img class="igem-logo" src="https://static.igem.org/mediawiki/2014/d/d8/Team_Berlin_igem_logo.png"></li></a>
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         <h2><img src="https://static.igem.org/mediawiki/2014/f/ff/Team_Berlin_igem_questionmark.png" alt="" class="teaser-icons hidden-xs" />Explore our Project:</h2>
         <h2><img src="https://static.igem.org/mediawiki/2014/f/ff/Team_Berlin_igem_questionmark.png" alt="" class="teaser-icons hidden-xs" />Explore our Project:</h2>
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              <a name="top">&nbsp;</a>
 
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              <a href="#description" class="sub-link-project"> 1. What is it all about?</a><br/>
 
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              <a href="#animation" class="sub-link-project">2. Animation</a><br/>
 
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              <a href="#detail-description" class="sub-link-project">3. Detailed description of the different strategies</a><br/>
 
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              <a href="#flow-chart" class="sub-link-project">4. Flow Chart with the strategies</a><br/>
 
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              <a href="#results" class="sub-link-project">5) Our Results</a><br/>
 
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              <a href="#lab-summary" class="sub-link-project">6. Lab-summary</a><br/>
 
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              <a href="#notebook" class="sub-link-project">7. Notebook</a><br/>
 
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         <!--<a href="#" class="more-informations-white">More informations</a>-->
         <!--<a href="#" class="more-informations-white">More informations</a>-->
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<!--- ////////////////// DESCRIPTION////////////////// -->
<!--- ////////////////// DESCRIPTION////////////////// -->
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              <a href="https://2014.igem.org/Team:Berlin/Project" class="sub-link-project"> 1. What is it all about?</a><br/><br/>
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        <ul>
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            <a href="https://2014.igem.org/Team:Berlin/Project/Activities" class="sub-link-project"> 2. Project-related Activities</a><br/><br/>
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          <li><a href="#top">GO TO TOP</a></li>
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              <a href="https://2014.igem.org/Team:Berlin/Project/Detailed-Description" class="sub-link-project">3. Detailed Description</a><br/><br/>
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              <a href="https://2014.igem.org/Team:Berlin/Project/Results" class="sub-link-project">4. Our Results</a><br/><br/>
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              <a href="https://2014.igem.org/Team:Berlin/Project/Summary" class="sub-link-project">5. Lab Summary</a><br/><br/>
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              <a href="https://2014.igem.org/Team:Berlin/Project/Journal" class="sub-link-project">6. Lab Journal</a><br/><br/>
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              <a href="https://2014.igem.org/Team:Berlin/Project/Property" class="sub-link-project">7. Intellectual Property</a><br/><br/>
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       <div class="project-number">1</div><div class="project-headline-float"><h2 class="green-text project-headline"> What is it all about?</h2></div>
       <div class="project-number">1</div><div class="project-headline-float"><h2 class="green-text project-headline"> What is it all about?</h2></div>
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         <a name="description">&nbsp;</a>
         <a name="description">&nbsp;</a>
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              As previous iGEM teams have shown, synthesizing fully functional magnetosomes in E. coli is highly difficult as more than 60 highly regulated genes are involved. As a more feasible alternative, we simply want to synthesize magnetic nanoparticles in E. coli in order to attract cells with strong magnetic fields.<br/>
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              Therefore we want to use different strategies including manipulation of the iron homeostasis of E. coli, expression of different metal binding proteins such as ferritins and metallothioneins as well as a high-throughput growth medium optimization.
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          <strong>iGEM Berlin 2014: A remote control for E. coli</strong><br/>
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              <br/><br/>
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<p>
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              Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis.  
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As the first iGEM team from Berlin, we decided to construct a simple BioBrick that enables synthetic biologists to remotely control the movement of E. coli. A seemingly simple and non-invasive mechanism for this remote control is the use of magnetic fields. By altering the iron homeostasis of E. coli, we want to increase the total iron level of the cytosol. By sequestering iron in a ferritin protein, iron crystals are formed and the cell is detoxified. We also worked with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to create various metal nanoparticles as an alternative strategy. Furthermore, we searched for the optimal conditions which yielded the most efficient formation of magnetic nanoparticles in E. coli. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used to remote control the cellular movement of E. coli.</p><br/>
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              Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.</p>
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          The following animation visualizes the concept of using ferritin iron storage proteins as a magnetism mediating module.<br/>
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<iframe src="//player.vimeo.com/video/109249906?byline=0&amp;portrait=0&amp;color=0ecd28" width="700" height="394" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe>
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<!--- ////////////////// ANIMATION ////////////////// -->
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           This animation was developed by Florian Renner who is an science interested and very talented graphic designer based in Munich. We thank Florian for his amazing job he did voluntarily for iGEM Berlin helping us to overcome the limitations of our budget.
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      <div class="project-number">2</div><div class="project-headline-float"><h2 class="green-text project-headline"> Visualisation</h2></div>
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              Animation, jo!
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<!--- ////////////////// DETAILED DESCRIPTION ////////////////// -->
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      <div class="project-number">3</div><div class="project-headline-float"><h2 class="green-text project-headline"> Detailes Description</h2></div>
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      <a name="detail-description">&nbsp;</a>
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              MORE DETAILS, jo!
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<!--- ////////////////// FLOW CHART ////////////////// -->
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      <div class="project-number">4</div><div class="project-headline-float"><h2 class="green-text project-headline"> Flow Chart</h2></div>
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              Flowchart, jo!
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<!--- ////////////////// Results ////////////////// -->
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      <div class="project-number">5</div><div class="project-headline-float"><h2 class="green-text project-headline"> The Results</h2></div>
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              Results, jo!
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<!--- ////////////////// Lab Diary ////////////////// -->
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      <div class="project-number">6</div><div class="project-headline-float"><h2 class="green-text project-headline"> Lab Summary</h2></div>
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                <h2 class="sub-content-project-headline">Week 1: 02.04.2014 – 06.04.2014</h2>
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                    Cultivation of E. coli Nissle 1917<br/>
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                    <br/>
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                    Genomic DNA extraction of E.coli Nissle strain<br/>
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                    Production of BfR, FTNA1 and FTNA2<br/>
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                    Restriction digest<br/>
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                    PCR Purification<br/>
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                    Ligation Assay<br/>
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                    Transformation into DH5α-Cells<br/>
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                <h2 class="sub-content-project-headline">Week 2: 07.04.2014 – 13.04.2014</h2>
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                    Colony PCR to check the results<br/>
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                    <br/>
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                    Bfr, FTNA 1, FTNA 2 primar designed for amplification<br/>
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                    <br/>
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                    Transformation of pQE_80L into DH5α<br/>
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                    <br/>
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                    Cultivation of BfR, FTNA 1, FTNA 2 in LB<br/>
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                <h2 class="sub-content-project-headline">Week 3: 14.04.2014 – 20.04.2014</h2>
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                    Miniprep of the cells from Week 2 <br/>
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                    <br/>
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                    DNA concentration determination and sequencing<br/>
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                    <br/>
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                    Expression of BfR, FTNA 1, FTNA 2 and induction with IPTG<br/>
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                    <br/>
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                    Production of a Mutaflor-Supression culture and streaking<br/>
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                <h2 class="sub-content-project-headline">Week 4: 21.04.2014 – 27. 04.2014 </h2>
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                    Minirep
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                <h2 class="sub-content-project-headline">Week 5: 28.04.2014 – 04.05.2014</h2>
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                    PCR Plasmid/Primar and Parameter check with Q5 Polymerase and Phusion Polymerase<br/>
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                    <br/>
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                    Miniprep of [pKD46+ DH5α] and [pKD4 + DH5 α]<br/>
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                    Genomic DNA extraction from Pseudomonas putida<br/>
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                    Enzyme digestion of different plasmids<br/>
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                <h2 class="sub-content-project-headline">Week 6: 05.05.2014 – 11.05.2014</h2>
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                    Preculture of strains from Budisa strain databse in LB medium and Midiprep<br/>
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                    <br/>
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                    Canamycin cassette PCR of pKD4<br/>
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                    <br/>
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                    Restriction digest of Plasmid pKD4 and pKD46v<br/>
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                <h2 class="sub-content-project-headline">Week 7: 12.05.2014 – 18.05.2014</h2>
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                    Biotransformation of Ferritin and pKD46 in Nissle and DH5 α strains
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                <h2 class="sub-content-project-headline">Week 8: 19.05.2014 – 25.05.2014</h2>
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                    Gel extraction of FieF PCR<br/>
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                    <br/>
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                    Gene Knockout of FieF in [RV + pKD46] and [WM10+pKD46]<br/>
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                    Transformation of pKD46 in Nissle and MG 1655<br/>
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                    PCR of ATPCS and PPMT<br/>
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                    Digestion of pQE_80L for cloning<br/>
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                <h2 class="sub-content-project-headline">Week 9: 26.05.2014 – 01.06.2014</h2>
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                    Production of LB plates<br/>
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                    AMB-1 and Microfluidic Chip were picked up from Max-Plank Institute<br/>
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                <h2 class="sub-content-project-headline">Week 10: 02.06.2014 – 08.06.2014</h2>
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                    Colony PCR and analytical gel electrophoresis for identifying the right clones<br/>
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                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
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Latest revision as of 21:59, 17 October 2014

Explore our Project:

1. What is it all about?

2. Project-related Activities

3. Detailed Description

4. Our Results

5. Lab Summary

6. Lab Journal

7. Intellectual Property


1

What is it all about?

  iGEM Berlin 2014: A remote control for E. coli

As the first iGEM team from Berlin, we decided to construct a simple BioBrick that enables synthetic biologists to remotely control the movement of E. coli. A seemingly simple and non-invasive mechanism for this remote control is the use of magnetic fields. By altering the iron homeostasis of E. coli, we want to increase the total iron level of the cytosol. By sequestering iron in a ferritin protein, iron crystals are formed and the cell is detoxified. We also worked with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to create various metal nanoparticles as an alternative strategy. Furthermore, we searched for the optimal conditions which yielded the most efficient formation of magnetic nanoparticles in E. coli. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used to remote control the cellular movement of E. coli.


The following animation visualizes the concept of using ferritin iron storage proteins as a magnetism mediating module.



This animation was developed by Florian Renner who is an science interested and very talented graphic designer based in Munich. We thank Florian for his amazing job he did voluntarily for iGEM Berlin helping us to overcome the limitations of our budget.

Müller-Breslau-Str. 10
10623 Berlin
Phone: +49 1778800244
Mail: mail@igem.berlin
©2014 iGem Berlin, Layout/Umsetzung: Valerian Blos, Marius Wessel

Retrieved from "http://2014.igem.org/Team:Berlin/Project"