Team:Berlin/Project

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   <a class="navmenu-brand" href="http://igem.berlin/index.php"><h1>iGEM Berlin</h1></a>
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    <h1 class="cover-heading green bubble">iGEM BERLIN</h1>
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      Remote-controlled E.coli bacteria?
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      That’s the aim of the interdisciplinary iGEM Team Berlin! <br />
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        <h2><img src="https://static.igem.org/mediawiki/2014/f/ff/Team_Berlin_igem_questionmark.png" alt="" class="teaser-icons hidden-xs" />Explore our Project:</h2>
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          <h2><img id="" src="https://static.igem.org/mediawiki/2014/f/ff/Team_Berlin_igem_questionmark.png" alt="" class="teaser-icons hidden-xs" />Magnetic e.Coli?</h2>
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          The bacteria shall be optimized for the absorption and synthesis of iron in order to
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          remote-control them magnetically. Small and efficient E.coli units with various areas
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          of use shall be the result.<br><br>These cells are attractive as they offer new solutions in bio therapeutics, bio materials and bio process engineering. Furthermore, we want to use these cells as remote controlled bacteria that can be controlled by a real time computer interface.</p>
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        <div id="ecoli-button" class="hidden-xs hidden-sm hidden-md"> <a class="btn-lg" ><img src="https://static.igem.org/mediawiki/2014/3/37/Team_Berlin_igem_arrow_down_white.png"> </a></div>
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              <a href="https://2014.igem.org/Team:Berlin/Project" class="sub-link-project"> 1. What is it all about?</a><br/><br/>
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            <a href="https://2014.igem.org/Team:Berlin/Project/Activities" class="sub-link-project"> 2. Project-related Activities</a><br/><br/>
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              <a href="https://2014.igem.org/Team:Berlin/Project/Detailed-Description" class="sub-link-project">3. Detailed Description</a><br/><br/>
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              <a href="https://2014.igem.org/Team:Berlin/Project/Results" class="sub-link-project">4. Our Results</a><br/><br/>
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              <a href="https://2014.igem.org/Team:Berlin/Project/Summary" class="sub-link-project">5. Lab Summary</a><br/><br/>
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              <a href="https://2014.igem.org/Team:Berlin/Project/Journal" class="sub-link-project">6. Lab Journal</a><br/><br/>
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      <div class="project-number">1</div><div class="project-headline-float"><h2 class="green-text project-headline"> What is it all about?</h2></div>
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          <h2><img src="https://static.igem.org/mediawiki/2014/f/ff/Team_Berlin_igem_questionmark.png" alt="" class="teaser-icons hidden-xs" />Project</h2>
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          <p>In October 2014, a team of Berlin students from a variety of backgrounds are participating
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          for the first time in the in the international genetically engineered machines competition.
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          For our project, the iGEM Berlin team has decided to make E. coli bacteria susceptible to
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          magnetic fields. By knocking out the iron efflux transporter (FieF) and iron uptake
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          suppressor (FUR), we want to increase the total iron level of the cytosol. By sequestering
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          iron in a ferritin protein, iron crystals are formed and the cell is detoxified. In order
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          to create ferromagnetic of super paramagnetic crystals, we will then use an intensive high
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          through put media optimization to discover the best conditions for the formation of magnetic
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          nanoparticles in E. coli. Furthermore, we will work with other metal binding proteins like
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          metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis in
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          E. coli. Once we have discovered the best way to magnetize E. coli bacteria we will build
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          and characterize a suitable BioBrick that can be used by any research lab or iGEM team in
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          the world in order to remote control the movement of E. coli cells.</p>
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          <h2><img src="https://static.igem.org/mediawiki/2014/f/ff/Team_Berlin_igem_questionmark.png" alt="" class="teaser-icons hidden-xs" />Team</h2>     
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          <p>The iGEM Team Berlin consists of 17 students from various universities in Berlin.
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          Represented are the Technische Universität Berlin, University of Applied Sciences Berlin,
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          Freie Universität as well as the Humboldt University. The team members come from diverse
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          disciplines. From biology, biotechnology, biochemistry, computer science, mathematics, mechanical
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          engineering to the history of science and technology, everything is represented. Just the
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          interdisciplinary nature of the team allows us to jointly develop solution strategies from
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          different perspectives. Hosted by Prof. Dr. Nediljko Budisa from the TU Berlin, our lab is
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          located at the Müller-Breslau-Straße 10, 10623 Berlin. Come by for a visit (Monday 5 pm).</p>
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          <a href="http://igem.berlin/team.php" class="more-informations-white hidden-xs hidden-sm hidden-md">More information</a>
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          <h2><img src="https://static.igem.org/mediawiki/2014/f/ff/Team_Berlin_igem_questionmark.png" alt="" class="teaser-icons hidden-xs" />Safety</h2>
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          <strong>iGEM Berlin 2014: A remote control for E. coli</strong><br/>
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          Our country uses a four-part „Safety Level“ rating system for laboratories. Level 4 is used for the most dangerous organisms.
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          This is equivalent to the WHO system. The Safety Level of our lab is Level 1 (low risk).
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As the first iGEM team from Berlin, we decided to construct a simple BioBrick that enables synthetic biologists to remotely control the movement of E. coli. A seemingly simple and non-invasive mechanism for this remote control is the use of magnetic fields. By altering the iron homeostasis of E. coli, we want to increase the total iron level of the cytosol. By sequestering iron in a ferritin protein, iron crystals are formed and the cell is detoxified. We also worked with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to create various metal nanoparticles as an alternative strategy. Furthermore, we searched for the optimal conditions which yielded the most efficient formation of magnetic nanoparticles in E. coli. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used to remote control the cellular movement of E. coli.</p><br/>
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          The following animation visualizes the concept of using ferritin iron storage proteins as a magnetism mediating module.<br/>
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            <!-- <p>Follow the activities around the iGEM Team Berlin. Interviews, reports and other news you will find here!</p> -->
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<iframe src="//player.vimeo.com/video/109249906?byline=0&amp;portrait=0&amp;color=0ecd28" width="700" height="394" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe>
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            <a href="http://igem.berlin/safety.php" class="more-informations-white hidden-xs hidden-sm hidden-md">More information</a>
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          <br/>
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          This animation was developed by Florian Renner who is an science interested and very talented graphic designer based in Munich. We thank Florian for his amazing job he did voluntarily for iGEM Berlin helping us to overcome the limitations of our budget.
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           <a href="http://igem.berlin/team.php"><li>Team |</li></a>           
           <a href="http://igem.berlin/safety.php">Safety |</li></a>
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           <a href="http://igem.berlin/workshop2014_neu.php"><li>Workshop |</li></a>
           <a href="http://igem.berlin/blog.php"><li>Blog</li></a>           
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Latest revision as of 21:59, 17 October 2014

Explore our Project:

1. What is it all about?

2. Project-related Activities

3. Detailed Description

4. Our Results

5. Lab Summary

6. Lab Journal

7. Intellectual Property


1

What is it all about?

  iGEM Berlin 2014: A remote control for E. coli

As the first iGEM team from Berlin, we decided to construct a simple BioBrick that enables synthetic biologists to remotely control the movement of E. coli. A seemingly simple and non-invasive mechanism for this remote control is the use of magnetic fields. By altering the iron homeostasis of E. coli, we want to increase the total iron level of the cytosol. By sequestering iron in a ferritin protein, iron crystals are formed and the cell is detoxified. We also worked with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to create various metal nanoparticles as an alternative strategy. Furthermore, we searched for the optimal conditions which yielded the most efficient formation of magnetic nanoparticles in E. coli. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used to remote control the cellular movement of E. coli.


The following animation visualizes the concept of using ferritin iron storage proteins as a magnetism mediating module.



This animation was developed by Florian Renner who is an science interested and very talented graphic designer based in Munich. We thank Florian for his amazing job he did voluntarily for iGEM Berlin helping us to overcome the limitations of our budget.

Müller-Breslau-Str. 10
10623 Berlin
Phone: +49 1778800244
Mail: mail@igem.berlin
©2014 iGem Berlin, Layout/Umsetzung: Valerian Blos, Marius Wessel

Retrieved from "http://2014.igem.org/Team:Berlin/Project"