A Three-Tiered Approach: Local, Regional and Global Outreach

For the human practices portion of our project, we wanted to continue the work of previous University of Virginia teams, while also pursuing a project unique to our current group. In other words, we wanted to do a lot this year. We decided to organize all of these projects into a multi-tiered system where the different tiers focused on the local, regional and international communities. Locally, we organized a Synthetic Biology Night at the University of Virginia, which was open to students from the University as well as locals interested in science and synthetic biology. Regionally, we continued to partner with Renaissance High School in downtown Charlottesville and began a new partnership with a public school in Albemarle County. We also met with Lynn Clements, the director of projects for the Rapidan Service Authority, to tour a regional wastewater treatment plant and discuss the need for and feasibility of our project’s implementation in wastewater treatment systems. Globally, we created a Synthetic Biology Awareness and Acceptance survey and asked iGEM teams to distribute it in their communities across the world.

The Panel

The event opened with a discussion of the ethical issues related to synthetic biology by three field experts, with the goal of informing the public and allaying any misconceptions about the topic. Dr. Kozminski, a molecular cell biologist, provided scientific background on the field of synthetic biology and brought a scientific perspective to the discussion. Dr. Arras, a professor in the bioethics department and member of President Obama’s Presidential Commission for the Study of Bioethical Issues, provided insight on ethical and religious groups’ perceptions of synthetic biology. Professor Bagley of the UVA Law School, shed some light on the legal side of synthetic biology research, especially issues concerning the patenting of genes modified or created using synthetic biology methods. Check out our event program to learn more about the panelists. Panelists discussed a range of topics from the environmental release of bacteria to the proper regulation of synthetic biology research. The conversation also touched on issues such as the labeling of synthetically produced products and the growth of DIY biology. The first portion of the dialogue was moderated by a member of our team, but the audience was encouraged to join in the dialogue and provide questions for the second portion of the panel. A reception followed the panel discussion, and attendees had a chance to chat with the panelists one on one, and to view posters submitted by local high schoolers (see below).

HIGH SCHOOL EDUCATION

Last year, the 2013 VGEM team reached out to Renaissance School and offered to teach a class on synthetic biology and the iGEM experience. Renaissance accepted, and the VGEM team visited the school once a week for six weeks during the fall. This pilot class has now become a regularly offered course at Renaissance School, and students taught by the 2014 VGEM competed at the 2014 High School iGEM competition. The work of the 2013 team inspired this year’s team to continue and expand their collaborative efforts with other schools in the community.

The Math, Engineering and Science Academy (MESA) at Albemarle High School
A New Partnership

We began the summer by reaching out to specialized science programs at public high schools in Albemarle County. We met with Jeff Prillaman, the director of the MESA program, to discuss teaching a synthetic biology crash course similar to our predecessors with his students. He accepted our offer, and we spent the summer preparing a three-day synthetic biology crash course to be presented to students in the program. Team members taught the synthetic biology course over the week of September 8th. The first class consisted of a general overview of basic molecular biology and an introduction to the field of synthetic biology; this curriculum was taught to both juniors (60) and seniors (40) in the MESA program. The second class focused on BioBrick formation and lab work. Seniors completed a restriction enzyme digest worksheet, loaded, ran and visualized a gel and transformed and plated bacteria containing plasmids with an RFP construct. The third class was split into two mini-lectures on the modeling used in synthetic biology and a discussion of the ethics involved in synthetic biology research. The students were given pre and post-assessments for this crash course.

Renaissance School
Advising a New Team

On October 8, 2014, we visited Renaissance School to give them feedback on their 2014 iGEM project and advise on their new project for the 2015 High School iGEM competition. We also presented students with awards from the What Can Synthetic Biology Do For You? competition.

Presentations and Protocols

          Day One: An Introduction to Synthetic Biology

          Day Two: A Day in the Life of an iGEMer

          Day Two: Lab Protocols

          Day Three (Part One): Modeling in Synthetic Biology

          Day Three (Part Two): The Ethics of Synthetic Biology

Worksheets

          Pre and Post Assessment

          Day Two: Lab Worksheet

Homework Reading

          VGEM Protocol Handbook

          Do-It-Yourself Biology? Messing Around with DNA Increasingly a Garage-Band Venture

          Your Vanilla Ice Cream Is About to Get Weirder

          Biohackers Are Kickstarting Some Unregulated Experiments

From Lab Bench to Implementation: Exploring the Viability of Our Biofilter

To see if our bacterial biofilter would be a viable solution to the issue of environmental microplastics, we met with Lynn Clements, the director of projects for the Rapidan Service Authority, at the Ruckersville wastewater treatment plant.

Making a Better Biofilter

After touring the plant and talking with Mr. Clements, we felt that our biofilter was a viable solution to the microplastics problem. We also now knew where best to implement the filter and had gathered parameters for a model-based assessment of our bacteria’s nylon degrading abilities in the plant.

Participants were asked to read the following short paragraph, and then rate their feelings towards synthetic biology:

All living things have genes. Genes determine how these living things, also known as organisms, look, what they do, and how they function. In synthetic biology, genes can be taken from one type of organism and put into another. This allows the modified organism to exhibit traits that it would not exhibit in nature. For instance, by putting a gene from a firefly into bacteria, you can make the bacteria glow.

They were given a scale from ‘strongly negative’ to ‘strongly positive.’ We then converted the responses to a Likert scale with a maximum value of 5 and a minimum value of 0.

Participants were later asked:

Would you drink water that is purified by bacteria if there are no bacteria in the water when you drink it, and the water is shown to be safe?

They were able to select either ‘yes’ or ‘no’ as responses. This map displays the percentage of ‘yes’ responses, leaving out either blank or ‘no’ responses. The maximum value is 10, and the minimum value is 0.

Comparing these maps shows a possible discrepancy between feelings towards synthetic biology in the abstract and applications of synthetic biology. Feelings toward synthetic biology appear to be, on average, neutral, but, for every region, at least 50% of participants responded that they would drink water cleaned through synthetic biological means, provided assured safety.

This shows promise that, with increased awareness of potential applications for synthetic biology, views may improve.

This year, we not only created and distributed a survey for our human practices project, but we also participated in several surveys sent to us by other iGEM teams. The following is a list of teams whose surveys we completed. Ben-Gurion University Israel Linkoping University Valencia Biocampus Virtus Parva Mexico ETH-Zurich Warwick Brasil SP

The First Annual Virginia iGEM Meet-Up

On Friday June 27th, we hosted our first meet-up for iGEM teams in the surrounding area. Members of the Rutgers, Duke and Georgia Tech teams came out to the meet up. We began with short presentations by each team of their projects for the summer. Teams then split into specialized discussion groups; these groups discussed topics such as the iGEM lab experience, human practices and community outreach, and finance. We ended the main portion of the event with a discussion of team collaboration. Some team members then joined us for dinner on the Corner and a night on the Downtown Mall.