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Team:CSU Fort Collins/Notebook/Breakdown/Aug
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| - | <title>Breakdown Notes - | + | <title>Breakdown Notes - August</title> |
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<ul> | <ul> | ||
<li><a href='/Team:CSU_Fort_Collins/Members/'><span>Members</span></a></li> | <li><a href='/Team:CSU_Fort_Collins/Members/'><span>Members</span></a></li> | ||
| - | <li class='last'><a href='/Team:CSU_Fort_Collins/ | + | <li><a href='/Team:CSU_Fort_Collins/Sponsors/'><span>Sponsors</span></a></li> |
| + | <li class='last'><a href='/Team:CSU_Fort_Collins/Acknowledgements/'><span>Acknowledgements</span></a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
| - | <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/'><span>Breakdown</span></a> | + | <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Jul'><span>Breakdown</span></a> |
<ul> | <ul> | ||
| - | <li><a href=" | + | <li><a href="/Team:CSU_Fort_Collins/Notebook/Breakdown/Jul"><span>July</span></a></li> |
| - | <li><a href=' | + | <li><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Aug'><span>August</span></a></li> |
| - | <li class='last'><a href=" | + | <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/Breakdown/Sep"><span>September</span></a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
| - | <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/HVP/'><span>High-Value Product</span></a> | + | <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/HVP/Jun'><span>High-Value Product</span></a> |
<ul> | <ul> | ||
| - | <li><a href=" | + | <li><a href="/Team:CSU_Fort_Collins/Notebook/HVP/Jun"><span>June</span></a></li> |
| - | + | <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/HVP/Jul"><span>July</span></a></li> | |
| - | + | ||
| - | <li class='last'><a href=" | + | |
</ul> | </ul> | ||
</li> | </li> | ||
| - | <li li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/KillSwitch/'><span>Kill Switch</span></a> | + | <li li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/KillSwitch/Jul'><span>Kill Switch</span></a> |
<ul> | <ul> | ||
| - | <li><a href=" | + | <li><a href="/Team:CSU_Fort_Collins/Notebook/KillSwitch/Jul"><span>July</span></a></li> |
| - | + | <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/KillSwitch/Sep"><span>September</span></a></li> | |
| - | <li class='last'><a href=" | + | |
</ul> | </ul> | ||
</li> | </li> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
| - | <li class='has-sub'><a href='/Team:CSU_Fort_Collins/ | + | <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Collab/'><span>Human Practices</span></a> |
<ul> | <ul> | ||
<li><a href='/Team:CSU_Fort_Collins/Collab/'><span>Collaboration</span></a></li> | <li><a href='/Team:CSU_Fort_Collins/Collab/'><span>Collaboration</span></a></li> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
| - | <li class='has-sub'><a href='/Team:CSU_Fort_Collins/ | + | <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Parts/'><span>Achievements</span></a> |
<ul> | <ul> | ||
<li><a href='/Team:CSU_Fort_Collins/Parts/'><span>Parts</span></a></li> | <li><a href='/Team:CSU_Fort_Collins/Parts/'><span>Parts</span></a></li> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
| - | <li><a href='/Team:CSU_Fort_Collins/Safety/'><span>Safety</span></a></li> | + | <li class='last'><a href='/Team:CSU_Fort_Collins/Safety/'><span>Safety</span></a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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| - | |||
<div class="clear"></div> | <div class="clear"></div> | ||
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<div class="month-group"> | <div class="month-group"> | ||
<a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Jul'>July</a> | <a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Jul'>July</a> | ||
| + | </div> | ||
<p>Lac Promoter and FadD Assembly</p> | <p>Lac Promoter and FadD Assembly</p> | ||
| - | |||
<div class="month-group"> | <div class="month-group"> | ||
<a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Aug'>August</a> | <a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Aug'>August</a> | ||
| - | <p> | + | </div> |
| - | + | <p>More of the Same, But Better</p> | |
<div class="month-group"> | <div class="month-group"> | ||
<a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Sep'>September</a> | <a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Sep'>September</a> | ||
| - | + | </div> | |
| - | + | <p>One Thousand and One Nights</p> | |
</div> | </div> | ||
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<div class='page'> | <div class='page'> | ||
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<h4>Tuesday, August 5</h4> | <h4>Tuesday, August 5</h4> | ||
| - | Reran PCR with correct primer using previously isolated E. coli genome. | + | Reran PCR with correct primer using previously isolated <u>E. coli</u> genome. |
<h4>Wednesday, August 6</h4> | <h4>Wednesday, August 6</h4> | ||
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<h4>Friday, August 8</h4> | <h4>Friday, August 8</h4> | ||
| - | Discussed with Sun why the PCR was not working. Discovered that when finding the melting temperatures we had not used the specifications of the polymerase we were using. Reran the PCR using a gradient around the new melting temperature. We used both colonies and previously isolated E. coli genome in different replicates for our template DNA. Ran a gel to determine success. The conditions which gave us the required 1.7 kb strand were 61 ºC and 1.7 minute extension step for the first five cycles and 72 ºC and 1.7 minute extension step for the last 30 cycles. The template DNA that worked came from the previously isolated E. coli genome. | + | Discussed with Sun why the PCR was not working. Discovered that when finding the melting temperatures we had not used the specifications of the polymerase we were using. Reran the PCR using a gradient around the new melting temperature. We used both colonies and previously isolated <u>E. coli</u> genome in different replicates for our template DNA. Ran a gel to determine success. The conditions which gave us the required 1.7 kb strand were 61 ºC and 1.7 minute extension step for the first five cycles and 72 ºC and 1.7 minute extension step for the last 30 cycles. The template DNA that worked came from the previously isolated <u>E. coli</u> genome.<br><br> |
| + | |||
| + | <center><img src='https://static.igem.org/mediawiki/2014/b/b7/Team-CSU_GelAug8.jpg' style='width:200px'/><br> | ||
| + | Gel Results, August 8</center> | ||
<h4>Monday, August 18</h4> | <h4>Monday, August 18</h4> | ||
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<h4>Tuesday, August 19</h4> | <h4>Tuesday, August 19</h4> | ||
| - | Transformed into E. coli to amplify the plasmid. | + | Transformed into <u>E. coli</u> to amplify the plasmid. |
<h4>Wednesday, August 20</h4> | <h4>Wednesday, August 20</h4> | ||
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<br><br> | <br><br> | ||
| - | <center><a href='/Team:CSU_Fort_Collins/Notebook/ | + | <center><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Jul' id='navi' style='margin-left:-20px; margin-right:40px'> Previous</a> <a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Sep' id='navi'>Next </a></center> |
</div> | </div> | ||
Latest revision as of 20:16, 17 October 2014
Breakdown Daily Notes
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Lac Promoter and FadD Assembly
More of the Same, But Better
One Thousand and One Nights
AUGUST
Friday, August 1
Discussed with Sun to troubleshoot why the PCR wasn’t working. We discovered that the final base in one of our primers was incorrect. We corrected that and reordered the primer.Tuesday, August 5
Reran PCR with correct primer using previously isolated E. coli genome.Wednesday, August 6
Checked PCR with gel, this gel failed. Reran PCR using a gradient of temperatures. Checked this PCR with a gel, which also failed.Thursday, August 7
Reran Gel with higher concentrations of PCR product. This gel also failed.Friday, August 8
Discussed with Sun why the PCR was not working. Discovered that when finding the melting temperatures we had not used the specifications of the polymerase we were using. Reran the PCR using a gradient around the new melting temperature. We used both colonies and previously isolated E. coli genome in different replicates for our template DNA. Ran a gel to determine success. The conditions which gave us the required 1.7 kb strand were 61 ºC and 1.7 minute extension step for the first five cycles and 72 ºC and 1.7 minute extension step for the last 30 cycles. The template DNA that worked came from the previously isolated E. coli genome.
Gel Results, August 8
