Team:Caltech/week4
From 2014.igem.org
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<b>Export Systems</b> | <b>Export Systems</b> | ||
<ul><li>PCR extracted vector backbone from pKS001 with Biobrick prefix & suffix overhangs</li> | <ul><li>PCR extracted vector backbone from pKS001 with Biobrick prefix & suffix overhangs</li> | ||
- | <li>PCR purification of PCR product and then 1 hour incubation at 37°C with DPN1 enzyme to remove any | + | <li>PCR purification of PCR product and then 1 hour incubation at 37°C with DPN1 enzyme to remove any unwanted fragments</li> |
<li>PCR purification of DPN1-digested product</li> | <li>PCR purification of DPN1-digested product</li> | ||
<li>Ran a gel that confirmed presence of ~3000bp-long DNA fragment in both DPN1-digested and undigested PCR products</li> | <li>Ran a gel that confirmed presence of ~3000bp-long DNA fragment in both DPN1-digested and undigested PCR products</li> |
Revision as of 05:07, 16 July 2014
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