Team:Aachen/OD/F device
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When building the OD/F Device, '''our goal''' was to develop a system that | When building the OD/F Device, '''our goal''' was to develop a system that | ||
- | * | + | * easy-to-handle |
- | * | + | * precise, stable, and reproducible results |
- | + | * portable | |
- | * | + | * easy to build from Open Source parts |
- | * | + | * combined measurement of optical density and fluorescence |
- | * | + | * low cost |
- | Commercially available equipment uses lasers and a set of two fine filters, one between laser and sample and one between sample and sensor. To reduce the cost, our OD/F Device uses a simpler measuring principle: it is designed with one low- | + | Commercially available equipment uses lasers and a set of two fine filters, one between laser and sample and one between sample and sensor. To reduce the cost, our OD/F Device uses a simpler measuring principle: it is designed with one low-cost filter, between sample and sensor, and illuminates with an LED instead of a laser. Nevertheless, one main goal was to produce an inexpensive device. Given that, we therefore had to compromise some of the measurement quality, were we still able to produce stable, precise and good data? |
{{Team:Aachen/Figure|Aachen_ODallstrains1.png|title=Transmission of different cell types at OD-values from 0.001-1|subtitle=The transmittance data of NIH 3T3 (mouse fibroblasts) cells align with the transmittance of ''P. putida'' and ''S. cerevisiae'' strains, even though the measured optical densities are lower by 1-2 orders of magnitude.|width=800px}} | {{Team:Aachen/Figure|Aachen_ODallstrains1.png|title=Transmission of different cell types at OD-values from 0.001-1|subtitle=The transmittance data of NIH 3T3 (mouse fibroblasts) cells align with the transmittance of ''P. putida'' and ''S. cerevisiae'' strains, even though the measured optical densities are lower by 1-2 orders of magnitude.|width=800px}} |
Revision as of 19:48, 17 October 2014
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