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Team:Tuebingen/Notebook/Protocols/Ni NTA Buffers
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<div id="TueContent"> | <div id="TueContent"> | ||
| - | + | <h1>Protocols</h1> | |
<h3>Ni NA buffers</h3> | <h3>Ni NA buffers</h3> | ||
| - | < | + | <h4> Lysis buffer (pH = 8)</h4> |
<table border="0"> | <table border="0"> | ||
<colgroup> | <colgroup> | ||
| Line 21: | Line 21: | ||
<tr> | <tr> | ||
<td style="text-align: center">50 mM</td> | <td style="text-align: center">50 mM</td> | ||
| - | <td> | + | <td>NaH<sub>2</sub>PO<sub>4</sub></td> |
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">300 mM</td> | ||
| + | <td>NaCl</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">10 mM</td> | ||
| + | <td>Imidazole</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | <p> </p> | ||
| + | |||
| + | <h4> Washing buffer (pH = 8)</h4> | ||
| + | <table border="0"> | ||
| + | <colgroup> | ||
| + | <col width="100"> | ||
| + | <col width="300"> | ||
| + | </colgroup> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">50 mM</td> | ||
| + | <td>NaH<sub>2</sub>PO<sub>4</sub></td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">300 mM</td> | ||
| + | <td>NaCl</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">20 mM</td> | ||
| + | <td>Imidazole</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | <p> </p> | ||
| + | |||
| + | <h4> Elution buffer (pH = 8)</h4> | ||
| + | <table border="0"> | ||
| + | <colgroup> | ||
| + | <col width="100"> | ||
| + | <col width="300"> | ||
| + | </colgroup> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">50 mM</td> | ||
| + | <td>NaH<sub>2</sub>PO<sub>4</sub></td> | ||
</tr> | </tr> | ||
| Line 31: | Line 81: | ||
<tr> | <tr> | ||
<td style="text-align: center">250 mM</td> | <td style="text-align: center">250 mM</td> | ||
| - | <td> | + | <td>Imidazole</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| + | |||
</div> | </div> | ||
Latest revision as of 19:41, 17 October 2014
Protocols
Ni NA buffers
Lysis buffer (pH = 8)
| 50 mM | NaH2PO4 |
| 300 mM | NaCl |
| 10 mM | Imidazole |
Washing buffer (pH = 8)
| 50 mM | NaH2PO4 |
| 300 mM | NaCl |
| 20 mM | Imidazole |
Elution buffer (pH = 8)
| 50 mM | NaH2PO4 |
| 300 mM | NaCl |
| 250 mM | Imidazole |
