"
Team:TU Darmstadt/Notebook/Methods/37 hydrochloric acid
From 2014.igem.org
(Difference between revisions)
| (One intermediate revision not shown) | |||
| Line 3: | Line 3: | ||
<html> | <html> | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
<div id="leftNavi" class="grid_5"> | <div id="leftNavi" class="grid_5"> | ||
| Line 16: | Line 13: | ||
<div id="wikicontent" class="grid_19"> | <div id="wikicontent" class="grid_19"> | ||
| - | <!--TYPO3SEARCH_begin--><div id="c246" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">37% hydrochloric acid extraction</h1></div><div class="csc-textpic-text"><div><p><b>Materials: </b></p></div><div><p><span style="font-size: 12px;">- 37% hydrochloric acid</span><br /><span style="font-size: 12px;">- Centrifuge</span><br /><span style="font-size: 12px;">- Tubes</span></p></div><div></div><div><p><b>Procedure:</b></p></div><div><p>Spin down the bacterial solution at 4500g for 10 minutes.</p></div><div><p>Remove culture media, weigh the remaining pellet and acetify with the same amount of 37% hydrochloric acid. </p></div><div><p>Spin down the resolved pellet at 13,3g for 10 minutes and Remove supernatant.</p></div><div><p>The anthocyanidin is present in the organic and the aqueous phase; the color can be changed by adjusting the pH-value. </p></div><div></div></div></div><!--TYPO3SEARCH_end--> | + | <!--TYPO3SEARCH_begin--><div id="c246" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">37% hydrochloric acid extraction</h1></div><div class="csc-textpic-text"><div><p><b>Materials: </b></p></div><div><p><span style="font-size: 12px;">- 37% hydrochloric acid</span><br /><span style="font-size: 12px;">- Centrifuge</span><br /><span style="font-size: 12px;">- Tubes</span></p></div><div></div><div><p><b>Procedure:</b></p></div><div><p>- Spin down the bacterial solution at 4500g for 10 minutes.</p></div><div><p>- Remove culture media, weigh the remaining pellet and acetify with the same amount of 37% hydrochloric acid. </p></div><div><p>- Spin down the resolved pellet at 13,3g for 10 minutes and Remove supernatant.</p></div><div><p>The anthocyanidin is present in the organic and the aqueous phase; the color can be changed by adjusting the pH-value. </p></div><div></div></div></div><!--TYPO3SEARCH_end--> |
</div> | </div> | ||
Latest revision as of 19:36, 17 October 2014
37% hydrochloric acid extraction
Materials:
- 37% hydrochloric acid
- Centrifuge
- Tubes
Procedure:
- Spin down the bacterial solution at 4500g for 10 minutes.
- Remove culture media, weigh the remaining pellet and acetify with the same amount of 37% hydrochloric acid.
- Spin down the resolved pellet at 13,3g for 10 minutes and Remove supernatant.
The anthocyanidin is present in the organic and the aqueous phase; the color can be changed by adjusting the pH-value.
