Team:Evry/Interlab Study/09-13-2014

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<h2>Constructions with other promoters</h2>
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<h2>Other constructions of the Anderson library of constitutive promoters</h2>
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<i>PSB1C3+E0240+J23101</i>:<br>
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pre-culture of the colony 3 in LB+Cam (1:1000). Incubation 16h at 37°C.
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<i>PSB1C3+E0240+J23105</i>, <i>PSB1C3+E0240+J23106</i> and <i>PSB1C3+E0240+J23107</i>:
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        <li> PCR colony of 7 colonies for each construction following protocol table 1 and 2.
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  <a href="https://static.igem.org/mediawiki/2014/8/8f/Gel106_107gfp.jpg" class="image">
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    <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/8/8f/Gel106_107gfp.jpg" width="450px;" class="thumbimage"/>
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    <center>GEL 1: 1% agarose gel. PCR product of 7 colonies of the PSB1C3+E0240+J23107 construction. PCR product of 8 colonies of the PSB1C3+E0240+J23106 construction. </center>
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A pre-culture was made with one colony for each construction to make a glycerol stock.<br>
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  <a href="https://static.igem.org/mediawiki/2014/c/c9/Gel105gfp.jpg" class="image">
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    <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/c/c9/Gel105gfp.jpg" width="450px;" class="thumbimage"/>
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    <center>GEL 2: 1% agarose gel. PCR product of 7 colonies of the PSB1C3+E0240+J23105 construction.</center>
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      </ul>
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A pre-culture was made with one colony for each construction to make a glycerol stock.<br>
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<span class="cd-date">Sep 13</span>  
<span class="cd-date">Sep 13</span>  

Latest revision as of 18:56, 17 October 2014

Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010



Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23101:
pre-culture of the colony 3 in LB+Cam (1:1000). Incubation 16h at 37°C.

PSB1C3+E0240+J23105, PSB1C3+E0240+J23106 and PSB1C3+E0240+J23107:
  • PCR colony of 7 colonies for each construction following protocol table 1 and 2.
    IMAGE
    Symbol
    GEL 1: 1% agarose gel. PCR product of 7 colonies of the PSB1C3+E0240+J23107 construction. PCR product of 8 colonies of the PSB1C3+E0240+J23106 construction.

    A pre-culture was made with one colony for each construction to make a glycerol stock.

    IMAGE
    Symbol
    GEL 2: 1% agarose gel. PCR product of 7 colonies of the PSB1C3+E0240+J23105 construction.

A pre-culture was made with one colony for each construction to make a glycerol stock.

Sep 13

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