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Team:ETH Zurich/lab/sequences
From 2014.igem.org
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[[File:ETH2014_piG0051max_Map.png|link=https://static.igem.org/mediawiki/2014/9/94/ETH2014_piG0051max.txt]] | [[File:ETH2014_piG0051max_Map.png|link=https://static.igem.org/mediawiki/2014/9/94/ETH2014_piG0051max.txt]] | ||
| - | == | + | ==Signal propagation constructs== |
| - | [https://static.igem.org/mediawiki/2014/ | + | [https://static.igem.org/mediawiki/2014/a/a7/ETH2014_piG0058_sensor_plasRstRBS-sfGFP.txt piG0058] |
| - | [[File: | + | [[File:ETH2014 piG0058 sensor plasRstRBS-sfGFP Map.png|https://static.igem.org/mediawiki/2014/a/a7/ETH2014_piG0058_sensor_plasRstRBS-sfGFP.txt]] |
| - | [https://static.igem.org/mediawiki/2014/ | + | [https://static.igem.org/mediawiki/2014/0/0f/ETH2014_piG0059_sensor_pluxRstRBS-sfGFP.txt piG0059] |
| - | [[File:ETH2014 | + | [[File:ETH2014 piG0059 sensor pluxRstRBS-sfGFP Map.png|https://static.igem.org/mediawiki/2014/0/0f/ETH2014_piG0059_sensor_pluxRstRBS-sfGFP.txt]] |
| - | [https://static.igem.org/mediawiki/2014/ | + | [https://static.igem.org/mediawiki/2014/2/22/PiG0060_sensor_prhlRstRBS-sfGFP.txt piG0060]] |
| - | [[File:ETH2014 | + | [[File:ETH2014 piG0060 sensor prhlRstRBS-sfGFP Map.png|https://static.igem.org/mediawiki/2014/2/22/PiG0060_sensor_prhlRstRBS-sfGFP.txt]] |
| - | [https://static.igem.org/mediawiki/2014/ | + | [https://static.igem.org/mediawiki/2014/2/2c/PiG0062_sensor_pluxRcr12yRBS-sfGFP.txt piG0062] |
| - | [[File:ETH2014 | + | [[File:ETH2014 piG0062 sensor pluxRcr12yRBS-sfGFP Map.png|https://static.igem.org/mediawiki/2014/2/2c/PiG0062_sensor_pluxRcr12yRBS-sfGFP.txt]] |
| - | [https://static.igem.org/mediawiki/2014/ | + | [https://static.igem.org/mediawiki/2014/d/da/ETH2014_piG0065_sensor_pluxRRR12y-sfGFP.txt piG0065] |
| - | [[File:ETH2014 | + | [[File:ETH2014 piG0065 sensor pluxRRR12y-sfGFP Map.png|https://static.igem.org/mediawiki/2014/d/da/ETH2014_piG0065_sensor_pluxRRR12y-sfGFP.txt]] |
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| + | [https://static.igem.org/mediawiki/2014/2/24/ETH2014_piG0066_sensor_prhlRRR12-sfGFP.txt piG0066] | ||
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| + | [[File:ETH2014 piG0066 sensor prhlRRR12-sfGFP Map.png|https://static.igem.org/mediawiki/2014/2/24/ETH2014_piG0066_sensor_prhlRRR12-sfGFP.txt]] | ||
[https://static.igem.org/mediawiki/2014/1/1d/ETH2014_piG0071_sensor_pluxRRR12y_bxb1_mCherry.txt piG0071] | [https://static.igem.org/mediawiki/2014/1/1d/ETH2014_piG0071_sensor_pluxRRR12y_bxb1_mCherry.txt piG0071] | ||
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[[File:ETH2014 piG0071 sensor pluxRRR12y bxb1 mCherry Map.png|https://static.igem.org/mediawiki/2014/1/1d/ETH2014_piG0071_sensor_pluxRRR12y_bxb1_mCherry.txt]] | [[File:ETH2014 piG0071 sensor pluxRRR12y bxb1 mCherry Map.png|https://static.igem.org/mediawiki/2014/1/1d/ETH2014_piG0071_sensor_pluxRRR12y_bxb1_mCherry.txt]] | ||
| - | [https://static.igem.org/mediawiki/2014/ | + | [https://static.igem.org/mediawiki/2014/f/f5/ETH2014_piG0096_pluxRRR12y-sfGFP-maxRBS-luxI.txt piG0096] |
| - | [[File:ETH2014 | + | [[File:ETH2014 piG0096 pluxRRR12y-sfGFP-maxRBS-luxI Map.png|https://static.igem.org/mediawiki/2014/f/f5/ETH2014_piG0096_pluxRRR12y-sfGFP-maxRBS-luxI.txt]] |
| - | [https://static.igem.org/mediawiki/2014/ | + | [https://static.igem.org/mediawiki/2014/f/fc/ETH2014_piG0097_pluxRRR12y-sfGFP-stRBS-luxI.txt piG0097] |
| - | [[File:ETH2014 | + | [[File:ETH2014 piG0097 pluxRRR12y-sfGFP-stRBS-luxI Map.png|https://static.igem.org/mediawiki/2014/f/fc/ETH2014_piG0097_pluxRRR12y-sfGFP-stRBS-luxI.txt]] |
| - | [https://static.igem.org/mediawiki/2014/ | + | [https://static.igem.org/mediawiki/2014/6/69/ETH2014_piG0099_pluxstRBS-sfGFP-stRBS-luxI.txt piG0099] |
| - | [[File: | + | [[File:ETH2014_piG0099_pluxstRBS-sfGFP-stRBS-luxI_Map.png|https://static.igem.org/mediawiki/2014/6/69/ETH2014_piG0099_pluxstRBS-sfGFP-stRBS-luxI.txt]] |
| - | [https://static.igem.org/mediawiki/2014/ | + | [https://static.igem.org/mediawiki/2014/9/95/ETH2014_piG0109_pluxRRR12y-sfGFP_EcoRI-_XbaI-.txt piG0109] |
| - | [[File:ETH2014 | + | [[File:ETH2014 piG0109 pluxRRR12y-sfGFP EcoRI- XbaI- Map.png|https://static.igem.org/mediawiki/2014/9/95/ETH2014_piG0109_pluxRRR12y-sfGFP_EcoRI-_XbaI-.txt]] |
| - | [https://static.igem.org/mediawiki/2014/ | + | [https://static.igem.org/mediawiki/2014/f/f9/ETH2014_piG0110_prhlRRR12-sfGFP_EcoRI-_XbaI-.txt piG0110] |
| - | [[File:ETH2014 | + | [[File:ETH2014 piG0110 prhlRRR12-sfGFP EcoRI- XbaI- Map.png|https://static.igem.org/mediawiki/2014/f/f9/ETH2014_piG0110_prhlRRR12-sfGFP_EcoRI-_XbaI-.txt]] |
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Revision as of 18:44, 17 October 2014
Sequences
The plasmid sequences can be accessed by clicking on the plasmid name (e.g. piG0040)
Regulator Constructs
LasR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LasR bound to 3OC12-HSL induces the expression of genes under the control of pLasR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 [15].
LuxR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 [15].
RhlR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 [15].
RhlR is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100] controlled by an RBS optimised for RhlR (RBS calculator). RhlR bound to C4-HSL induces the expression of genes under the control of pRhlR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 [15].
LuxR is expressed under the weak constitutive promoter [http://parts.igem.org/Part:BBa_J23109 BBa_J23109] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 [15].
LuxR is expressed under the medium strong constitutive promoter [http://parts.igem.org/Part:BBa_J23111 BBa_J23111 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxR bound to 3OC6-HSL induces the expression of genes under the control of pLuxR. The plasmid pBR322 and its derivatives have a copy number of 15 to 20 [15].
Producer Constructs
LasI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LasI produces the quorum sensing molecule 3OC12-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [16].
LasI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by an RBS optimised for LasI (RBS calculator). LasI produces the quorum sensing molecule 3OC12-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [16].
LuxI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [16].
LuxI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by an RBS optimised for LuxI (RBS calculator). LuxI produces the quorum sensing molecule 3OC6-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [16].
RhlI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034]. RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [16].
RhlI is expressed under the strong constitutive promoter [http://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] controlled by an RBS optimised for RhlI (RBS calculator). RhlI produces the quorum sensing molecule C4-HSL. The pBBR1 origin is present at a copy number of approximately 5, however, the origin is poorly characterized [16].
Signal propagation constructs
