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Team:Paris Saclay/Notebook/July/10
From 2014.igem.org
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==Lab work== | ==Lab work== | ||
| - | + | ===1 - Plasmid DNA Extraction:=== | |
| - | ''Romain | + | ''by Romain and Sean'' |
Plasmids used: | Plasmids used: | ||
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol] | ||
| - | + | ===2 - Transformation of competent cells E.coli=== | |
| - | ''Fabio | + | ''by Fabio and Maher'' |
Competent strains used: | Competent strains used: | ||
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*MG1655Z1 | *MG1655Z1 | ||
*DH5α+ (given by Solenne) | *DH5α+ (given by Solenne) | ||
| - | (The strains DH5α, MG1655 and MG1655Z1 were prepared by | + | (''The strains DH5α, MG1655 and MG1655Z1 were prepared by Mathias and Mathieu'') |
BioBricks used: | BioBricks used: | ||
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*J23100, J23106, J23114: without the BioBrick, nothing brows. | *J23100, J23106, J23114: without the BioBrick, nothing brows. | ||
| - | Results: | + | '''Results:''' |
*pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well. | *pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well. | ||
*J23100, J23106, J23114: The strain DH5α+ grow well and the 3 others strains grow less. They have to be more concentrated. | *J23100, J23106, J23114: The strain DH5α+ grow well and the 3 others strains grow less. They have to be more concentrated. | ||
Revision as of 15:13, 15 July 2014
Contents |
Thursday 10th July
Lab work
1 - Plasmid DNA Extraction:
by Romain and Sean
Plasmids used:
- BBa_B0015, dble ter
- FNR_RBS_amilCP-Ter DH5αZ1
2 - Transformation of competent cells E.coli
by Fabio and Maher
Competent strains used:
- DH5α
- MG1655
- MG1655Z1
- DH5α+ (given by Solenne)
(The strains DH5α, MG1655 and MG1655Z1 were prepared by Mathias and Mathieu)
BioBricks used:
- J23100 (Amp R)(Dilutions: Concentrated, 0 and 10^-1)
- J23106 (Amp R)(Dilutions: Concentrated, 0 and 10^-1)
- J23114 (Amp R)(Dilutions: Concentrated, 0 and 10^-1)
+ pET28a (Kan R)(Dilutions: 0, 10^-1 and 10^-2) (pET28a is a plasmid used to verify that the 4 strains are competent).
The negative controles:
- pET28a: without plasmid, nothing grows.
- J23100, J23106, J23114: without the BioBrick, nothing brows.
Results:
- pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well.
- J23100, J23106, J23114: The strain DH5α+ grow well and the 3 others strains grow less. They have to be more concentrated.
